Mouse Anti-CYP4F11 Recombinant Antibody (F21 P6 F5) (CBMAB-C4814WJ)

This product is a Mouse antibody that recognizes CYP4F11. This antibody F21 P6 F5 can be used for immunoassay techniques such as: ELISA, IHC-P, WB.
See all CYP4F11 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

F21 P6 F5

Antibody Isotype

IgG1

Application

ELISA, IHC-P, WB

Basic Information

Immunogen

Synthetic peptide corresponding to human CYP450 4F11

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.08% sodium azide

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

Cytochrome P450 Family 4 Subfamily F Member 11

Introduction

CYP4F11 (Cytochrome P450 Family 4 Subfamily F Member 11) is a Protein Coding gene. Among its related pathways are Arachidonic acid metabolism and Metabolism. Gene Ontology (GO) annotations related to this gene include iron ion binding and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen. An important paralog of this gene is CYP4F2.

Entrez Gene ID

57834

UniProt ID

Q9HBI6

Alternative Names

Cytochrome P450 Family 4 Subfamily F Member 11; Cytochrome P450, Family 4, Subfamily F, Polypeptide 11; 3-Hydroxy Fatty Acids Omega-Hydroxylase CYP4F11; Cytochrome P450, Subfamily IVF, Polypeptide 11; Cytochrome P450 4F11; CYPIVF11; Phylloquinone Omega-Hydroxylase CYP4F11; EC 1.14.13.194; EC 1.14.13.-;

Function

A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids and their oxygenated derivatives (oxylipins) (PubMed:24138531, PubMed:15364545, PubMed:18065749).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:15364545, PubMed:18065749, PubMed:24138531).

Catalyzes with high efficiency the oxidation of the terminal carbon (omega-oxidation) of 3-hydroxy fatty acids, such as 3-hydroxyhexadecanoic and 3-hydroxyoctadecanoic acids, likely participating in the biosynthesis of long-chain 3-hydroxydicarboxylic acids (PubMed:18065749, PubMed:19932081).

Omega-hydroxylates and inactivates phylloquinone (vitamin K1), and menaquinone-4 (MK-4, a form of vitamin K2), both acting as cofactors in blood coagulation (PubMed:24138531).

Metabolizes with low efficiciency fatty acids, including (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) and its oxygenated metabolite 8-hydroxyeicosatetraenoic acid (8-HETE) (PubMed:15364545, PubMed:19932081).

Catalyzes N- and O-demethylation of drugs such as erythromycin, benzphetamine, ethylmorphine, chlorpromazine, imipramine and verapamil (PubMed:15364545).

Biological Process

Arachidonic acid metabolic process Source: GO_Central
Blood coagulation Source: UniProtKB
Fatty acid metabolic process Source: BHF-UCL
Leukotriene B4 catabolic process Source: GO_Central
Menaquinone catabolic process Source: UniProtKB
Oxylipin biosynthetic process Source: UniProtKB-UniPathway
Phylloquinone catabolic process Source: UniProtKB
Vitamin K catabolic process Source: UniProtKB

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane

Topology

Helical: 15-37

References

Skorokhod, O., Correddu, D., Catucci, G., & Gianfranco, G. (2021). Posttranslational modifications of CYP4F11 by 4-hydroxynonenal inhibit enzyme ω-hydroxylation function: putative role in malaria immunosuppression. In 61st SIB Congress Virtual Edition (pp. 343-343). SIB.

Yi, M., Parvez, M. M., Cho, S. A., Kim, D. H., Jung, J. A., Lee, S. J., & Shin, J. G. (2017). Identification of Functionally Defective CYP4F11 Genetic Variants in Erythromycin Metabolism and 20-Hete Synthesis. Clinical Therapeutics, 39(8), e61.

Yi, M., Cho, S. A., Min, J., Kim, D. H., Shin, J. G., & Lee, S. J. (2017). Functional characterization of a common CYP4F11 genetic variant and identification of functionally defective CYP4F11 variants in erythromycin metabolism and 20-HETE synthesis. Archives of biochemistry and biophysics, 620, 43-51.

Zhang, J. E., Klein, K., Jorgensen, A. L., Francis, B., Alfirevic, A., Bourgeois, S., ... & Pirmohamed, M. (2017). Effect of genetic variability in the CYP4F2, CYP4F11, and CYP4F12 genes on liver mRNA levels and warfarin response. Frontiers in pharmacology, 8, 323.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-CYP4F11 Recombinant Antibody (CBCNC-571)

Rabbit Anti-CYP4F11 Recombinant Antibody (EG909)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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