Sign in or Register   Sign in or Register
  |  

Mouse Anti-CYP4Z1 Recombinant Antibody (CBXC-3155) (CBMAB-C0953-CQ)

This product is a mouse antibody that recognizes CYP4Z1. The antibody CBXC-3155 can be used for immunoassay techniques such as: WB, ELISA, IHC.
See all CYP4Z1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXC-3155
Antibody Isotype
IgG1, κ
Application
WB, ELISA, IHC

Basic Information

Immunogen
Ovalbumin-conjugated synthetic peptide KLAPDHSRPP (C-terminal sequence)
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Cytochrome P450 Family 4 Subfamily Z Member 1
Introduction
CYP4Z1 (Cytochrome P450 Family 4 Subfamily Z Member 1) is a Protein Coding gene. Among its related pathways are Cytochrome P450 - arranged by substrate type. Gene Ontology (GO) annotations related to this gene include iron ion binding and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen. An important paralog of this gene is CYP4X1.
Entrez Gene ID
199974
UniProt ID
Q86W10
Alternative Names
Cytochrome P450 Family 4 Subfamily Z Member 1; Cytochrome P450, Family 4, Subfamily Z, Polypeptide 1; CYPIVZ1; Cytochrome P450 4Z1; EC 1.14.14.1; CYP4A20;
Function
A cytochrome P450 monooxygenase that catalyzes the in-chain oxidation of fatty acids (PubMed:19090726, PubMed:29018033).

Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates lauric and myristic acids predominantly at the omega-4 and omega-2 positions, respectively (PubMed:19090726, PubMed:29018033).

Catalyzes the epoxidation of double bonds of polyunsaturated fatty acids (PUFA). Displays an absolute stereoselectivity in the epoxidation of arachidonic acid producing the 14(S),15(R)-epoxyeicosatrienoic acid (EET) enantiomer (PubMed:29018033).

Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:19090726, PubMed:29018033).
Cellular Location
Endoplasmic reticulum membrane; Microsome membrane
Topology
Cytoplasmic: 1-9
Helical: 10-30
Lumenal: 31-505

Khayeka-Wandabwa, C., Zhao, J., Pathak, J. L., Wu, H., & Bureik, M. (2022). Upregulation of estrogen receptor alpha (ERα) expression in transgenic mice expressing human CYP4Z1. Breast cancer research and treatment, 191(2), 319-326.

Machalz, D., Li, H., Du, W., Sharma, S., Liu, S., Bureik, M., & Wolber, G. (2021). Discovery of a novel potent cytochrome P450 CYP4Z1 inhibitor. European Journal of Medicinal Chemistry, 215, 113255.

Al-Saraireh, Y. M., Alshammari, F. O., Youssef, A. M., Al-Sarayreh, S., Almuhaisen, G. H., Alnawaiseh, N., ... & Alrawashdeh, H. M. (2021). Profiling of CYP4Z1 and CYP1B1 expression in bladder cancers. Scientific Reports, 11(1), 1-8.

Liu, H., Qin, H., Zhou, Y., Yuan, Y., Liu, Y., Chen, Y., ... & Zheng, L. (2021). HET0016 attenuates the stemness of breast cancer cells through targeting CYP4Z1. Molecular Carcinogenesis, 60(6), 413-426.

Du, W., Machalz, D., Yan, Q., Sorensen, E. J., Wolber, G., & Bureik, M. (2020). Importance of asparagine-381 and arginine-487 for substrate recognition in CYP4Z1. Biochemical Pharmacology, 174, 113850.

Zheng, L., Guo, Q., Xiang, C., Liu, S., Jiang, Y., Gao, L., ... & Xi, T. (2019). Transcriptional factor six2 promotes the competitive endogenous RNA network between CYP4Z1 and pseudogene CYP4Z2P responsible for maintaining the stemness of breast cancer cells. Journal of hematology & oncology, 12(1), 1-18.

Khayeka-Wandabwa, C., Ma, X., Cao, X., Nunna, V., Pathak, J. L., Bernhardt, R., ... & Bureik, M. (2019). Plasma membrane localization of CYP4Z1 and CYP19A1 and the detection of anti-CYP19A1 autoantibodies in humans. International immunopharmacology, 73, 64-71.

Nunna, V., Jalal, N., & Bureik, M. (2017). Anti-CYP4Z1 autoantibodies detected in breast cancer patients. Cellular & molecular immunology, 14(6), 572-574.

Yan, Q., Machalz, D., Zöllner, A., Sorensen, E. J., Wolber, G., & Bureik, M. (2017). Efficient substrate screening and inhibitor testing of human CYP4Z1 using permeabilized recombinant fission yeast. Biochemical pharmacology, 146, 174-187.

Yang, X., Hutter, M., Wen Bin Goh, W., & Bureik, M. (2017). CYP4Z1–a human cytochrome P450 enzyme that might hold the key to curing breast cancer. Current pharmaceutical design, 23(14), 2060-2064.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-CYP4Z1 Recombinant Antibody (CBXC-3155)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare