Mouse Anti-DARS Recombinant Antibody (CBYCD-153) (CBMAB-D0242-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Aspartyl-TRNA Synthetase (DARS). The antibody can be used for immunoassay techniques, such as ELISA, IHC-P, RNAi, WB.
See all DARS antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBYCD-153

Antibody Isotype

IgG2a, κ

Application

ELISA, IHC-P, RNAi, WB

Basic Information

Immunogen

DARS (NP_001340, 393 a.a. ~ 501 a.a) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

aspartyl-tRNA synthetase

Introduction

DARS belongs to a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity.

Entrez Gene ID

1615

UniProt ID

P14868

Alternative Names

Aspartyl-TRNA Synthetase; Cell Proliferation-Inducing Gene 40 Protein; Aspartate TRNA Ligase 1, Cytoplasmic; EC 6.1.1.12; AspRS; Aspartyl-TRNA Synthetase, Cytoplasmic; Aspartate--TRNA Ligase, Cytoplasmic; Testicular Tissue Protein Li 192; HBSL;

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.

Biological Process

Aspartyl-tRNA aminoacylation Source: GO_Central
Protein-containing complex assembly Source: ProtInc
Translation Source: ProtInc
tRNA aminoacylation for protein translation Source: Reactome

Cellular Location

Cytosol

Involvement in disease

Hypomyelination with brainstem and spinal cord involvement and leg spasticity (HBSL):
An autosomal recessive leukoencephalopathy characterized by onset in the first year of life of severe spasticity, mainly affecting the lower limbs and resulting in an inability to achieve independent ambulation. Affected individuals show delayed motor development and nystagmus; some may have mild mental retardation. Brain MRI shows hypomyelination and white matter lesions in the cerebrum, brainstem, cerebellum, and spinal cord.

References

Jiao, M., Guo, H., Chen, Y., Li, L., & Zhang, L. (2020). DARS-AS1 promotes clear cell renal cell carcinoma by sequestering miR-194-5p to up-regulate DARS. Biomedicine & Pharmacotherapy, 128, 110323.

Dou, B., Jiang, Z., Chen, X., Wang, C., Wu, J., An, J., & Sheng, G. (2020). Oncogenic long noncoding RNA DARS-AS1 in childhood acute myeloid leukemia by binding to microRNA-425. Technology in Cancer Research & Treatment, 19, 1533033820965580.

Chen, Y., Wu, Q., Lin, J., & Wei, J. (2020). DARS-AS1 accelerates the proliferation of cervical cancer cells via miR-628-5p/JAG1 axis to activate Notch pathway. Cancer Cell International, 20(1), 1-11.

Fröhlich, D., Suchowerska, A. K., Voss, C., He, R., Wolvetang, E., Von Jonquieres, G., ... & Klugmann, M. (2018). Expression pattern of the aspartyl-tRNA synthetase DARS in the human brain. Frontiers in Molecular Neuroscience, 11, 81.

Fröhlich, D., Suchowerska, A. K., Spencer, Z. H., von Jonquieres, G., Klugmann, C. B., Bongers, A., ... & Klugmann, M. (2017). In vivo characterization of the aspartyl-tRNA synthetase DARS: homing in on the leukodystrophy HBSL. Neurobiology of disease, 97, 24-35.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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