Mouse Anti-DAZAP1 Recombinant Antibody (2242C2a) (CBMAB-D0272-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DAZ Associated Protein 1 (DAZAP1). The antibody can be used for immunoassay techniques, such as WB, Dot.
See all DAZAP1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

2242C2a

Antibody Isotype

IgG2b

Application

WB, Dot

Basic Information

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

DAZ associated protein 1

Introduction

DAZAP1 is a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis.

Entrez Gene ID

26528

UniProt ID

Q96EP5

Alternative Names

DAZ Associated Protein 1; Deleted In Azoospermia Associated Protein 1; Deleted In Azoospermia-Associated Protein 1;

Function

RNA-binding protein, which may be required during spermatogenesis.

Biological Process

Cell differentiation Source: UniProtKB-KW
Cell population proliferation Source: Ensembl
Maternal placenta development Source: Ensembl
Positive regulation of mRNA splicing, via spliceosome Source: GO_Central
Spermatogenesis Source: GO_Central

Cellular Location

Nucleus; Cytoplasm. Predominantly cytoplasmic. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm (By similarity).

PTM

Acetylation at Lys-150 is predominantly observed in the nuclear fraction, and may regulate nucleocytoplasmic transport.

References

Wang, Q., Guo, Y., Wang, W., Liu, B., Yang, G., Xu, Z., ... & Liu, Z. (2021). RNA binding protein DAZAP1 promotes HCC progression and regulates ferroptosis by interacting with SLC7A11 mRNA. Experimental Cell Research, 399(1), 112453.

Chen, Y., Lu, Y., Ren, Y., Yuan, J., Zhang, N., Kimball, H., ... & Yang, M. (2020). Starvation-induced suppression of DAZAP1 by miR-10b integrates splicing control into TSC2-regulated oncogenic autophagy in esophageal squamous cell carcinoma. Theranostics, 10(11), 4983.

Bo, W. E. N. G., RAN, M. L., Rong, C., PENG, F. Z., Hui, L. U. O., Hu, G. A. O., ... & Bin, C. H. E. N. (2019). miR-10b promotes porcine immature Sertoli cell proliferation by targeting the DAZAP1 gene. Journal of Integrative Agriculture, 18(8), 1924-1935.

Sasaki, K., Ono, M., Takabe, K., Suzuki, A., & Kurihara, Y. (2018). Specific intron-dependent loading of DAZAP1 onto the cox6c transcript suppresses pre-mRNA splicing efficacy and induces cell growth retardation. Gene, 657, 1-8.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-DAZAP1 Recombinant Antibody (2F6)

Mouse Anti-DAZAP1 Recombinant Antibody (CBYCD-161)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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