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Mouse Anti-DCLRE1B Recombinant Antibody (2B7) (CBMAB-D0365-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DNA Cross-Link Repair 1B (DCLRE1B). The antibody can be used for immunoassay techniques, such as FC, IHC-P, WB.
See all DCLRE1B antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2B7
Antibody Isotype
IgG1
Application
FC, IHC-P, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 1-280

Target

Full Name
DNA Cross-Link Repair 1B
Introduction
DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links.
Entrez Gene ID
64858
UniProt ID
Q9H816
Alternative Names
DNA Cross-Link Repair 1B; DNA Cross-Link Repair 1B (PSO2 Homolog, S. Cerevisiae); SNM1 Homolog B; APOLLO; SNM1B; SNMIB; DNA Cross-Link Repair 1B Protein;
Function
5'-3' exonuclease that plays a central role in telomere maintenance and protection during S-phase. Participates in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair, thereby ensuring that telomeres do not fuse. Plays a key role in telomeric loop (T loop) formation by being recruited by TERF2 at the leading end telomeres and by processing leading-end telomeres immediately after their replication via its exonuclease activity: generates 3' single-stranded overhang at the leading end telomeres avoiding blunt leading-end telomeres that are vulnerable to end-joining reactions and expose the telomere end in a manner that activates the DNA repair pathways. Together with TERF2, required to protect telomeres from replicative damage during replication by controlling the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Also involved in response to DNA damage: plays a role in response to DNA interstrand cross-links (ICLs) by facilitating double-strand break formation. In case of spindle stress, involved in prophase checkpoint.
Biological Process
Double-strand break repair via nonhomologous end joining Source: GO_Central
Interstrand cross-link repair Source: BHF-UCL
Mitotic cell cycle checkpoint Source: UniProtKB
Protection from non-homologous end joining at telomere Source: UniProtKB
Telomere capping Source: BHF-UCL
Telomere maintenance Source: UniProtKB
Telomere maintenance via telomere lengthening Source: BHF-UCL
Telomeric 3' overhang formation Source: UniProtKB
Telomeric loop formation Source: UniProtKB
Cellular Location
Nucleus; Centrosome; Telomere. Mainly localizes to telomeres, recruited via its interaction with TERF2. During mitosis, localizes to the centrosome.
Involvement in disease
Hoyeraal-Hreidarsson syndrome (HHS):
The gene represented in this entry may be involved in disease pathogenesis. An aberrant splice variant of DCLRE1B, designated Apollo-Delta, has been found in a patient with Hoyeraal-Hreidarsson syndrome (PubMed:20479256). Apollo-Delta hampers the proper replication of telomeres, leading to major telomeric dysfunction and cellular senescence, but maintains its DNA interstrand cross-link repair function in the whole genome. A clinically severe variant of dyskeratosis congenita that is characterized by multisystem involvement, early onset in utero, and often results in death in childhood. Affected individuals show intrauterine growth retardation, microcephaly, cerebellar hypoplasia, delayed development, and bone marrow failure resulting in immunodeficiency.
PTM
Ubiquitinated, leading to its degradation. Interaction with TERF2 protects it from ubiquitination.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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