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Mouse Anti-DDO Recombinant Antibody (3F7) (CBMAB-D0495-YC)

Provided herein is a Mouse monoclonal antibody, which binds to D-Aspartate Oxidase (DDO). The antibody can be used for immunoassay techniques, such as ELISA, WB.
See all DDO antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3F7
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
DDO (NP_003640, 270 a.a. ~ 369 a.a) partial recombinant protein with GST tag. The immunogen sequence: WNLSPDAENS REILSRCCAL EPSLHGACNI REKVGLRPYR PGVRLQTELL ARDGQRLPVV HHYGHGSGGI SVHWGTALEA ARLVSECVHA LRTPIPKSNL
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
aa 270-369

Target

Full Name
D-aspartate oxidase
Introduction
DDO is a peroxisomal flavoprotein that catalyzes the oxidative deamination of D-aspartate and N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction.
Entrez Gene ID
8528
UniProt ID
Q99489
Alternative Names
D-Aspartate Oxidase; EC 1.4.3.1; DASOX; D-Aspartate Oxidase, DDO; Aspartic Oxidase; DDO-1; DDO-2;
Function
Selectively catalyzes the oxidative deamination of D-aspartate and its N-methylated derivative, N-methyl D-aspartate.
Biological Process
Aspartate catabolic process Source: UniProtKB
Carboxylic acid metabolic process Source: Reactome
D-amino acid catabolic process Source: UniProtKB
Grooming behavior Source: Ensembl
Hormone metabolic process Source: Ensembl
Insemination Source: Ensembl
Protein localization Source: Reactome
Cellular Location
Peroxisome

Katane, M., Kuwabara, H., Nakayama, K., Saitoh, Y., Miyamoto, T., Sekine, M., & Homma, H. (2020). Biochemical characterization of d-aspartate oxidase from Caenorhabditis elegans: Its potential use in the determination of free d-glutamate in biological samples. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1868(8), 140442.

Takahashi, S. (2020). d-Aspartate oxidase: Distribution, functions, properties, and biotechnological applications. Applied microbiology and biotechnology, 104(7), 2883-2895.

De Rosa, A., Mastrostefano, F., Di Maio, A., Nuzzo, T., Saitoh, Y., Katane, M., ... & Errico, F. (2020). Prenatal expression of d-aspartate oxidase causes early cerebral d-aspartate depletion and influences brain morphology and cognitive functions at adulthood. Amino Acids, 52(4), 597-617.

Nuzzo, T., Feligioni, M., Cristino, L., Pagano, I., Marcelli, S., Iannuzzi, F., ... & Usiello, A. (2019). Free d-aspartate triggers NMDA receptor-dependent cell death in primary cortical neurons and perturbs JNK activation, Tau phosphorylation, and protein SUMOylation in the cerebral cortex of mice lacking d-aspartate oxidase activity. Experimental neurology, 317, 51-65.

Katane, M., Kuwabara, H., Nakayama, K., Saitoh, Y., Miyamoto, T., Sekine, M., & Homma, H. (2018). Rat d-aspartate oxidase is more similar to the human enzyme than the mouse enzyme. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1866(7), 806-812.

Katane, M., Kanazawa, R., Kobayashi, R., Oishi, M., Nakayama, K., Saitoh, Y., ... & Homma, H. (2017). Structure–function relationships in human D-aspartate oxidase: characterisation of variants corresponding to known single nucleotide polymorphisms. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics, 1865(9), 1129-1140.

Sacchi, S., Novellis, V. D., Paolone, G., Nuzzo, T., Iannotta, M., Belardo, C., ... & Usiello, A. (2017). Olanzapine, but not clozapine, increases glutamate release in the prefrontal cortex of freely moving mice by inhibiting D-aspartate oxidase activity. Scientific reports, 7(1), 1-13.

Nuzzo, T., Sacchi, S., Errico, F., Keller, S., Palumbo, O., Florio, E., ... & Usiello, A. (2017). Decreased free d-aspartate levels are linked to enhanced d-aspartate oxidase activity in the dorsolateral prefrontal cortex of schizophrenia patients. npj Schizophrenia, 3(1), 1-10.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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