Sign in or Register   Sign in or Register
  |  

Mouse Anti-DDOST Recombinant Antibody (2B4) (CBMAB-D0496-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Non-Catalytic Subunit (DDOST). The antibody can be used for immunoassay techniques, such as WB, IHC.
See all DDOST antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2B4
Antibody Isotype
IgG1
Application
WB, IHC

Basic Information

Immunogen
Human recombinant protein fragment corresponding to amino acids 131-378 of human DDOST (NP_005207) produced in E.coli
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
dolichyl-diphosphooligosaccharide-protein glycosyltransferase
Introduction
DDOST is a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. TDDOST is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.
Entrez Gene ID
1650
UniProt ID
P39656
Alternative Names
Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Non-Catalytic Subunit; Advanced Glycation End-Product Receptor 1; Oligosaccharyl Transferase 48 KDa Subunit; Oligosaccharyltransferase Subunit 48; OST48; Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit (Non-Catalytic); Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 48 KDa Subunit; Dolichyl-Diphosphooligosaccharide-Protein Glycosyltransferase; Dolichyl-Diphosphooligosaccharide-Protein Glycotransferase; Advanced Glycation Endproduct Receptor 1; Oligosaccharyltransferase 48 KDa Subunit;
Function
Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Required for the assembly of both SST3A- and SS3B-containing OST complexes. Required for efficient N-glycosylation.

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc3Man9GlcNAc2 in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity).

Required for the assembly of both SST3A- and SS3B-containing OST complexes (PubMed:22467853).
Biological Process
Neutrophil degranulation Source: Reactome
Protein glycosylation Source: UniProtKB
Protein N-linked glycosylation Source: UniProtKB
Protein N-linked glycosylation via asparagine Source: GO_Central
Regulation of protein stability Source: ARUK-UCL
Response to cytokine Source: UniProtKB
T cell activation Source: UniProtKB
Viral protein processing Source: Reactome
Cellular Location
Endoplasmic reticulum membrane
Involvement in disease
Congenital disorder of glycosylation 1R (CDG1R):
A form of congenital disorder of glycosylation, a multisystem disorder caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions.
Topology
Lumenal: 43-427
Helical: 428-447
Cytoplasmic: 448-456
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-DDOST Recombinant Antibody (2B4)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare