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Mouse Anti-DDX1 Recombinant Antibody (22/DDX1) (CBMAB-D0524-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DEAD-Box Helicase 1 (DDX1). The antibody can be used for immunoassay techniques, such as WB, IF.
See all DDX1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
22/DDX1
Antibody Isotype
IgG1
Application
WB, IF

Basic Information

Immunogen
Human DDX1 a.a.351-544
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DEAD (Asp-Glu-Ala-Asp) box polypeptide 1
Introduction
DDX1 is a DEAD box protein of unknown function. It shows high transcription levels in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.
Entrez Gene ID
1653
UniProt ID
Q92499
Alternative Names
DEAD-Box Helicase 1; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 1; DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 1; DEAD (Asp-Glu-Ala-Asp) Box Helicase 1; DEAD Box Protein Retinoblastoma; DEAD Box Protein 1; DBP-RB; ATP-Dependent RNA Helicase DDX1;
Function
Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation (PubMed:24870230).

Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1. Specifically binds (via helicase ATP-binding domain) on both short and long poly(I:C) dsRNA (By similarity).
(Microbial infection) Required for HIV-1 Rev function as well as for HIV-1 and coronavirus IBV replication. Binds to the RRE sequence of HIV-1 mRNAs.
(Microbial infection) Required for Coronavirus IBV replication.
Biological Process
Defense response to virus Source: UniProtKB-KW
DNA duplex unwinding Source: UniProtKB
Double-strand break repair Source: UniProtKB
Innate immune response Source: UniProtKB-KW
Multicellular organism development Source: UniProtKB
Nucleic acid phosphodiester bond hydrolysis Source: UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Protein localization to cytoplasmic stress granule Source: AgBase
Regulation of translational initiation Source: UniProtKB
Spliceosomal complex assembly Source: UniProtKB
tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB
Viral process Source: UniProtKB-KW
Cellular Location
Cytoplasm; Cytosol; Nucleus; Mitochondrion; Cytoplasmic granule. Localized with MBNL1, TIAL1 and YBX1 in stress granules upon stress. Localized with CSTF2 in cleavage bodies. Forms large aggregates called DDX1 bodies. Relocalized into multiple foci (IR-induced foci or IRIF) after IR treatment, a process that depends on the presence of chromosomal DNA and/or RNA-DNA duplexes. Relocalized at sites of DNA double-strand breaks (DSBs) in an ATM-dependent manner after IR treatment. Colocalized with RELA in the nucleus upon TNF-alpha induction. Enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low (PubMed:24608264). Colocalizes in the cytosol with DDX21, DHX36 and TICAM1. Colocalizes in the mitochondria with TICAM1 and poly(I:C) RNA ligand. The multi-helicase-TICAM1 complex may translocate to the mitochondria upon poly(I:C) stimulation (By similarity).
Cytoplasm. (Microbial infection) Relocalized to the cytoplasm with a perinuclear staining pattern in avian infectious bronchitis virus (IBV)-infected cells (PubMed:20573827). Required for proper localization of HIV-1 Rev (PubMed:15567440).
PTM
Phosphorylated by ATM kinase; phosphorylation is increased in response to ionizing radiation (IR).

Jin, Y., Shi, J., Wang, H., Lu, J., Chen, C., Yu, Y., ... & Guo, Y. (2021). MYC-associated protein X binding with the variant rs72780850 in RNA helicase DEAD box 1 for susceptibility to neuroblastoma. Science China Life Sciences, 64(6), 991-999.

Taschuk, F., & Cherry, S. (2020). DEAD-box helicases: sensors, regulators, and effectors for antiviral defense. Viruses, 12(2), 181.

Mojzesz, M., Klak, K., Wojtal, P., Adamek, M., Podlasz, P., Chmielewska-Krzesinska, M., ... & Rakus, K. (2020). Viral infection-induced changes in the expression profile of non-RLR DExD/H-box RNA helicases (DDX1, DDX3, DHX9, DDX21 and DHX36) in zebrafish and common carp. Fish & shellfish immunology, 104, 62-73.

Xue, Q., Liu, H., Zeng, Q., Zheng, H., Xue, Q., & Cai, X. (2019). The dead-box RNA helicase DDX1 interacts with the viral protein 3D and inhibits foot-and-mouth disease virus replication. Virologica Sinica, 34(6), 610-617.

Meier-Stephenson, V., Mrozowich, T., Pham, M., & Patel, T. R. (2018). DEAD-box helicases: the Yin and Yang roles in viral infections. Biotechnology and Genetic Engineering Reviews, 34(1), 3-32.

Tanaka, K., Ikeda, N., Miyashita, K., Nuriya, H., & Hara, T. (2018). DEAD box protein DDX 1 promotes colorectal tumorigenesis through transcriptional activation of the LGR5 gene. Cancer Science, 109(8), 2479-2489.

Lamichhane, R., Hammond, J. A., Pauszek, R. F., Anderson, R. M., Pedron, I., van der Schans, E., ... & Millar, D. P. (2017). A DEAD-box protein acts through RNA to promote HIV-1 Rev-RRE assembly. Nucleic acids research, 45(8), 4632-4641.

Hammond, J. A., Lamichhane, R., Millar, D. P., & Williamson, J. R. (2017). A DEAD-box helicase mediates an RNA structural transition in the HIV-1 rev response element. Journal of molecular biology, 429(5), 697-714.

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For research use only. Not intended for any clinical use.

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