Rabbit Anti-DDX19B Recombinant Antibody (EG938) (CBMAB-EN1114-LY)

The product is antibody recognizes DDX19B. The antibody EG938 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:10000.
See all DDX19B antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse

Clone

EG938

Antibody Isotype

IgG

Application

WB: 1:500~1:1000 ELISA: 1:10000

Basic Information

Immunogen

The antibody was produced against synthesized peptide derived from internal of human DDX19B.

Specificity

Human, Mouse

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

DEAD-Box Helicase 19B

Introduction

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Entrez Gene ID

Human	11269
Mouse	234733

UniProt ID

Human	Q9UMR2
Mouse	Q8BZY3

Alternative Names

DEAD-Box Helicase 19B; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 19 (Dbp5, Yeast, Homolog); DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 19B; DEAD Box Protein 19B; DDX19; DBP5; DEAD (Asp-Glu-Ala-As) Box Polypeptide 19B; DEAD (Asp-Glu-Ala-As) Box Polypeptide 19; ATP-Dependent RNA Helicase DDX19B; ATP-Dependent RNA Helicase DDX19;

Function

ATP-dependent RNA helicase involved in mRNA export from the nucleus (PubMed:10428971).

Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins (PubMed:10428971).

Biological Process

mRNA export from nucleus Source: ProtInc
Poly(A)+ mRNA export from nucleus Source: GO_Central

Cellular Location

Nucleoplasm; Cytoplasm. Associates with the nuclear pore complex cytoplasmic fibrils.

References

Park, Y., Park, J., Hwang, H. J., Kim, L., Jeong, K., Song, H. K., ... & Kim, Y. K. (2021). Translation mediated by the nuclear cap-binding complex is confined to the perinuclear region via a CTIF–DDX19B interaction. Nucleic acids research, 49(14), 8261-8276.

Mamoor, S. (2020). DDX19B is differentially expressed in the brains of patients with psychotic disorders.

Zhang, K., Zhang, Y., Xue, J., Meng, Q., Liu, H., Bi, C., ... & Weng, C. (2019). DDX19 inhibits type I interferon production by disrupting TBK1-IKKε-IRF3 interactions and promoting TBK1 and IKKε degradation. Cell reports, 26(5), 1258-1272.

Okamura, M., Yamanaka, Y., Shigemoto, M., Kitadani, Y., Kobayashi, Y., Kambe, T., ... & Masuda, S. (2018). Depletion of mRNA export regulator DBP5/DDX19, GLE1 or IPPK that is a key enzyme for the production of IP6, resulting in differentially altered cytoplasmic mRNA expression and specific cell defect. PLoS One, 13(5), e0197165.

Mikhailova, T., Shuvalova, E., Ivanov, A., Susorov, D., Shuvalov, A., Kolosov, P. M., & Alkalaeva, E. (2017). RNA helicase DDX19 stabilizes ribosomal elongation and termination complexes. Nucleic acids research, 45(3), 1307-1318.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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