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Mouse Anti-DDX4 Recombinant Antibody (CBYCD-219) (CBMAB-D0567-YC)

Provided herein is a Mouse monoclonal antibody, which binds to DEAD-Box Helicase 4 (DDX4). The antibody can be used for immunoassay techniques, such as WB, IHC-P, IHC-Fr, ICC, IF.
See all DDX4 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
CBYCD-219
Antibody Isotype
IgG1
Application
WB, IHC-P, IHC-Fr, ICC, IF

Basic Information

Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
DEAD-Box Helicase 4
Introduction
DDX4 is a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. DDX4 is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.
Entrez Gene ID
Human54514
Mouse13206
UniProt ID
HumanQ9NQI0
MouseQ61496
Alternative Names
DEAD-Box Helicase 4; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 4; DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 4; DEAD Box Protein 4; Vasa Homolog; VASA; Probable ATP-Dependent RNA Helicase DDX4; EC 3.6.4.13; EC 3.6.1;
Function
ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600).

Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity).

Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity).

Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (By similarity).

Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (By similarity).
Biological Process
Cell differentiation Source: GO_Central
DNA methylation involved in gamete generation Source: UniProtKB
Flagellated sperm motility Source: UniProtKB
Gamete generation Source: GO_Central
Gene silencing by RNA Source: UniProtKB-KW
Male meiosis I Source: UniProtKB
Male meiotic nuclear division Source: UniProtKB
Multicellular organism development Source: UniProtKB-KW
Negative regulation of transposition Source: UniProtKB
PiRNA biosynthetic process Source: UniProtKB
PiRNA metabolic process Source: UniProtKB
Spermatogenesis Source: UniProtKB
Cellular Location
Cytoplasm; Perinuclear region. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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