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Mouse Anti-DGKE Recombinant Antibody (CBYCD-252) (CBMAB-D0785-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Diacylglycerol Kinase Epsilon (DGKE). The antibody can be used for immunoassay techniques, such as WB, IHC.
See all DGKE antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYCD-252
Antibody Isotype
IgG2b
Application
WB, IHC

Basic Information

Immunogen
E. coli-derived recombinant human DGK-epsilon, Asn314-Arg435, Accession # P52429
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
diacylglycerol kinase, epsilon 64kDa
Introduction
Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGKE is expressed predominantly in testis.
Entrez Gene ID
Human8526
Mouse56077
Rat497978
UniProt ID
HumanP52429
MouseQ9R1C6
RatQ2YDU7
Alternative Names
Diacylglycerol Kinase Epsilon; Diacylglycerol Kinase, Epsilon 64kDa; Diglyceride Kinase Epsilon; DAG Kinase Epsilon; EC 2.7.1.107; DGK-Epsilon; DAGK5;
Function
Membrane-bound diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:15544348, PubMed:19744926, PubMed:22108654, PubMed:21477596, PubMed:23949095).

Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:8626589, PubMed:15544348).

Also plays an important role in the biosynthesis of complex lipids (PubMed:8626589).

Displays specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with the highest activity toward 1-octadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycerol the main diacylglycerol intermediate within the phosphatidylinositol turnover cycle (PubMed:19744926, PubMed:22108654, PubMed:23274426).

Can also phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position but much less efficiently (PubMed:22108654).
Biological Process
Diacylglycerol metabolic process Source: UniProtKB
Intracellular signal transduction Source: GO_Central
Lipid phosphorylation Source: UniProtKB
Modulation of chemical synaptic transmission Source: Ensembl
Phosphatidic acid biosynthetic process Source: UniProtKB
Phosphatidylinositol biosynthetic process Source: Ensembl
Platelet activation Source: Reactome
Protein kinase C-activating G protein-coupled receptor signaling pathway Source: InterPro
Cellular Location
Cytoplasm; Membrane
Involvement in disease
Nephrotic syndrome 7 (NPHS7):
A form of nephrotic syndrome, a renal disease clinically characterized by severe proteinuria, resulting in complications such as hypoalbuminemia, hyperlipidemia and edema. NPHS7 is an autosomal recessive form characterized by onset of proteinuria usually in the first decade of life. The disorder is progressive, and some patients develop end-stage renal disease within several years. Renal biopsy typically shows membranoproliferative glomerulonephritis.
Hemolytic uremic syndrome atypical 7 (AHUS7):
An atypical form of hemolytic uremic syndrome characterized by acute onset in the first year of life of microangiopathic hemolytic anemia, thrombocytopenia, and renal failure. After the acute episode, most patients develop chronic renal insufficiency. Unlike other genetic forms of aHUS, AHUS7 is not related to abnormal activation of the complement system.

Alabdulqader, M., & Alfakeeh, K. (2021). A patient with a homozygous diacylglycerol kinase epsilon (DGKE) gene mutation with atypical haemolytic uraemic syndrome and low C3 responded well to eculizumab: a case report. BMC nephrology, 22(1), 1-5.

Koyun, M., Aksoy, G. K., Çomak, E., Özaltın, F., & Akman, S. (2021). Outcome of diacylglycerol kinase epsilon–mediated hemolytic uremic syndrome in an infant. Kidney International, 99(6), 1500-1501.

Liu, D., Ding, Q., Dai, D. F., Padhy, B., Nayak, M. K., Li, C., ... & Attanasio, M. (2021). Loss of diacylglycerol kinase ε causes thrombotic microangiopathy by impairing endothelial VEGFA signaling. JCI insight, 6(9).

Fu, W., Li, M., Lin, H., Xu, Y., Han, W., Chen, H., & Sun, L. (2021). Cyclosporine A relieved proteinuria and hypoproteinemia in DGKE nephropathy. Clinica Chimica Acta, 518, 78-82.

Brocklebank, V., Kumar, G., Howie, A. J., Chandar, J., Milford, D. V., Craze, J., ... & Kavanagh, D. (2020). Long-term outcomes and response to treatment in diacylglycerol kinase epsilon nephropathy. Kidney international, 97(6), 1260-1274.

Holanda, M. I. D., Gomes, C. P., Araujo, S. D. A., Wanderley, D. C., Eick, R. G., Dantas, G. C., ... & Palma, L. M. P. (2019). Diacylglycerol kinase epsilon nephropathy: late diagnosis and therapeutic implications. Clinical kidney journal, 12(5), 641-644.

Sharma, A., Khandelwal, P., Yadav, M., Sethi, S., Hari, P., Sinha, A., & Bagga, A. (2019). Atypical hemolytic uremic syndrome with diacylglycerol kinase epsilon (DGKE) gene mutation. Asian Journal of Pediatric Nephrology, 2(2), 101.

So, V., Wu, J., Traynor-Kaplan, A., Choy, C., Epand, R., Botelho, R., & Lemaire, M. (2019). Phosphatidylinositol cycle disruption is central to atypical hemolytic-uremic syndrome caused by diacylglycerol kinase epsilon deficiency. bioRxiv, 633867.

Azukaitis, K., Simkova, E., Majid, M. A., Galiano, M., Benz, K., Amann, K., ... & Schaefer, F. (2017). The phenotypic spectrum of nephropathies associated with mutations in diacylglycerol kinase ε. Journal of the American Society of Nephrology, 28(10), 3066-3075.

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For research use only. Not intended for any clinical use.

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