Mouse Anti-DIS3L2 Recombinant Antibody (D0940) (V2LY-0425-LY365)

Name: Mouse Anti-DIS3L2 Recombinant Antibody (D0940)
SKU: V2LY-0425-LY365
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

D0940

Antibody Isotype

IgG1

Application

WB, IHC-P, FC, ELISA

Basic Information

Immunogen

Purified recombinant fragment of human DIS3L2 (aa27-250) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:10,000
WB	1:500-1:2,000
IHC	1:200-1:1,000
FC	1:200-1:400

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium Azide

Concentration

1 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

DIS3 LIKE 3'-5' EXORIBONUCLEASE 2

Entrez Gene ID

129563

UniProt ID

Q8IYB7

Function

3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of both mRNAs and miRNAs that have been polyuridylated by a terminal uridylyltransferase, such as ZCCHC11/TUT4. Mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'. Mediates degradation of uridylated pre-let-7 miRNAs, contributing to the maintenance of embryonic stem (ES) cells. Essential for correct mitosis, and negatively regulates cell proliferation.

Biological Process

Cell division Source: UniProtKB-KW
miRNA catabolic process Source: UniProtKB
Mitotic cell cycle Source: UniProtKB
Mitotic sister chromatid separation Source: UniProtKB
mRNA catabolic process Source: GO_Central
Negative regulation of cell population proliferation Source: UniProtKB
Nuclear-transcribed mRNA catabolic process, exonucleolytic Source: UniProtKB
Nuclear-transcribed mRNA catabolic process, exonucleolytic, 3'-5' Source: InterPro
Polyuridylation-dependent mRNA catabolic process Source: UniProtKB
Stem cell population maintenance Source: UniProtKB

Cellular Location

Cytoplasm; P-body

Involvement in disease

Perlman syndrome (PRLMNS):
An autosomal recessive congenital overgrowth syndrome. Affected children are large at birth, are hypotonic, and show organomegaly, characteristic facial dysmorphisms (inverted V-shaped upper lip, prominent forehead, deep-set eyes, broad and flat nasal bridge, and low-set ears), renal anomalies (nephromegaly and hydronephrosis), frequent neurodevelopmental delay, and high neonatal mortality. Perlman syndrome is associated with a high risk of Wilms tumor. Histologic examination of the kidneys in affected children shows frequent nephroblastomatosis, which is a precursor lesion for Wilms tumor.

More Infomation

References

Ballou, E. R., Cook, A. G., & Wallace, E. W. (2021). Repeated evolution of inactive pseudonucleases in a fungal branch of the Dis3/RNase II family of nucleases. Molecular Biology and Evolution, 38(5), 1837-1846.

Towler, B. P., Pashler, A. L., Haime, H. J., Przybyl, K. M., Viegas, S. C., Matos, R. G., ... & Newbury, S. F. (2020). Dis3L2 regulates cell proliferation and tissue growth through a conserved mechanism. PLoS genetics, 16(12), e1009297.

Luan, S., Luo, J., Liu, H., & Li, Z. (2019). Regulation of RNA decay and cellular function by 3′-5′ exoribonuclease DIS3L2. RNA biology, 16(2), 160-165.

Xing, S., Li, Z., Ma, W., He, X., Shen, S., Wei, H., ... & Zhang, H. (2019). DIS3L2 promotes progression of hepatocellular carcinoma via hnRNP U-mediated alternative splicing. Cancer Research, 79(19), 4923-4936.

Saramago, M., Costa, P. J. D., Viegas, S. C., & Arraiano, C. M. (2019). The implication of mRNA degradation disorders on human DISease: focus on DIS3 and DIS3-like enzymes. The mRNA Metabolism in Human Disease, 85-98.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-DIS3L2 Recombinant Antibody (14i132)

Mouse Anti-DIS3L2 Recombinant Antibody (6C7B2)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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SDS
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