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Mouse Anti-DNASE1 Recombinant Antibody (13j29) (CBMAB-D1338-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Deoxyribonuclease 1 (DNASE1). The antibody can be used for immunoassay techniques, such as ELISA, ICC, IHC, WB.
See all DNASE1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
13j29
Antibody Isotype
IgG
Application
ELISA, ICC, IHC, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Deoxyribonuclease 1
Introduction
DNASE1 belongs to the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Entrez Gene ID
1773
UniProt ID
P24855
Alternative Names
Deoxyribonuclease 1; Deoxyribonuclease I; Dornase Alfa; EC 3.1.21.1; DNL1; DRNI;
Function
Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed:2251263, PubMed:11241278, PubMed:2277032).

Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity).

Among other functions, seems to be involved in cell death by apoptosis (PubMed:11241278).

Binds specifically to G-actin and blocks actin polymerization (By similarity).

Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity).

NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity).

Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).
Biological Process
Apoptotic process Source: UniProtKB-KW
DNA catabolic process Source: GO_Central
DNA catabolic process, endonucleolytic Source: UniProtKB
Neutrophil activation involved in immune response Source: UniProtKB
Regulation of acute inflammatory response Source: UniProtKB
Regulation of neutrophil mediated cytotoxicity Source: UniProtKB
Cellular Location
Secreted; Nucleus envelope; Zymogen granule. Secretory protein, stored in zymogen granules and found in the nuclear envelope.
Involvement in disease
Systemic lupus erythematosus (SLE):
Disease susceptibility is associated with variants affecting the gene represented in this entry. Neutrophil extracellular traps (NETs) are impaired in patients suffering from SLE (PubMed:20439745). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (PubMed:20439745). A chronic, relapsing, inflammatory, and often febrile multisystemic disorder of connective tissue, characterized principally by involvement of the skin, joints, kidneys and serosal membranes. It is of unknown etiology, but is thought to represent a failure of the regulatory mechanisms of the autoimmune system. The disease is marked by a wide range of system dysfunctions, an elevated erythrocyte sedimentation rate, and the formation of LE cells in the blood or bone marrow.

Egli-Spichtig, D., Zhang, M. Y., Li, A., Pastor Arroyo, E. M., Hernando, N., Wagner, C. A., ... & Perwad, F. (2021). Renal Dnase1 expression is regulated by FGF23 but loss of Dnase1 does not alter renal phosphate handling. Scientific reports, 11(1), 1-14.

Engavale, M., McCord, J., Mapp, B., Nzimulinda, N., Bengtson, E., Sutton, R. B., & Keyel, P. A. (2021). Dnase1 Family in Autoimmunity. Encyclopedia, 1(3), 527-541.

Verhülsdonk, L., Mannherz, H. G., & Napirei, M. (2021). Comparison of the secretory murine DNase1 family members expressed in Pichia pastoris. Plos one, 16(7), e0253476.

Han, D. S., Ni, M., Chan, R. W., Chan, V. W., Lui, K. O., Chiu, R. W., & Lo, Y. D. (2020). The biology of cell-free DNA fragmentation and the roles of DNASE1, DNASE1L3, and DFFB. The American Journal of Human Genetics, 106(2), 202-214.

Kenny, E. F., Raupach, B., Abu Abed, U., Brinkmann, V., & Zychlinsky, A. (2019). Dnase1‐deficient mice spontaneously develop a systemic lupus erythematosus‐like disease. European Journal of Immunology, 49(4), 590-599.

Cheng, T. H., Lui, K. O., Peng, X. L., Cheng, S. H., Jiang, P., Chan, K. A., ... & Lo, Y. D. (2018). DNase1 does not appear to play a major role in the fragmentation of plasma DNA in a knockout mouse model. Clinical chemistry, 64(2), 406-408.

Pranzatelli, T. J., Michael, D. G., & Chiorini, J. A. (2018). ATAC2GRN: optimized ATAC-seq and DNase1-seq pipelines for rapid and accurate genome regulatory network inference. BMC genomics, 19(1), 1-13.

Boettcher, M., Meier, D., Jiménez-Alcázar, M., Eschenburg, G., Mietzsch, S., Vincent, D., ... & Fuchs, T. A. (2017). Degradation of extracellular DNA by DNase1 significantly reduces testicular damage after testicular torsion in rats. Urology, 109, 223-e1.

Gatselis, N. K., Vakrakou, A. G., Zachou, K., Androutsakos, T., Azariadis, K., Hatzis, G., ... & Dalekos, G. N. (2017). Decreased serum DNase1-activity in patients with autoimmune liver diseases. Autoimmunity, 50(2), 125-132.

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For research use only. Not intended for any clinical use.

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