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Mouse Anti-DPYS Recombinant Antibody (3B1) (CBMAB-D1646-YC)

Provided herein is a Mouse monoclonal antibody, which binds to Dihydropyrimidinase (DPYS). The antibody can be used for immunoassay techniques, such as ELISA.
See all DPYS antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3B1
Antibody Isotype
IgG1, κ
Application
ELISA

Basic Information

Immunogen
DPYS, a.a. 422-519 partial recombinant protein with GST tag.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
dihydropyrimidinase
Introduction
DPYS catalyzes the conversion of 5,6-dihydrouracil to 3-ureidopropionate in pyrimidine metabolism. DPYS is expressed at a high level in liver and kidney as a major 2.5-kb transcript and a minor 3.8-kb transcript. Defects in the DPYS gene are linked to dihydropyrimidinuria.
Entrez Gene ID
1807
UniProt ID
Q14117
Alternative Names
Dihydropyrimidinase; Dihydropyrimidine Amidohydrolase; Hydantoinase; EC 3.5.2.2; DHPase; DHP;
Function
Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyze the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate.
Biological Process
Beta-alanine metabolic process Source: Ensembl
Pyrimidine nucleobase catabolic process Source: UniProtKB
Thymine catabolic process Source: UniProtKB
Uracil catabolic process Source: UniProtKB
Cellular Location
Cytosol; Extracellular exosome; Protein-containing complex
Involvement in disease
Dihydropyrimidinase deficiency (DPYSD):
An autosomal recessive disorder of pyrimidine metabolism characterized by dihydropyrimidinuria. It is associated with a variable clinical phenotype characterized by epileptic or convulsive attacks, dysmorphic features and severe developmental delay, and congenital microvillous atrophy. Most patients are, however, asymptomatic.
PTM
Carboxylation allows a single lysine to coordinate two zinc ions.

Hishinuma, E., Gutiérrez Rico, E., & Hiratsuka, M. (2020). In Vitro Assessment of Fluoropyrimidine-Metabolizing Enzymes: Dihydropyrimidine Dehydrogenase, Dihydropyrimidinase, and β-Ureidopropionase. Journal of Clinical Medicine, 9(8), 2342.

Mirzaei, M., Kavosi, A., Sharifzadeh, M., Mahjoub, G., Faghihi, M. A., Habibzadeh, P., & Yavarian, M. (2020). A novel stop-gain mutation in DPYS gene causing Dihidropyrimidinase deficiency, a case report. BMC medical genetics, 21(1), 1-7.

Shu, J., Cai, F., Xu, X., Zhang, X., Wang, X., Zheng, J., ... & Zhang, Y. (2020). Analysis of gene variant in a Chinese child affected with dihydropyrimidinase deficiency. Zhonghua yi xue yi Chuan xue za zhi= Zhonghua Yixue Yichuanxue Zazhi= Chinese Journal of Medical Genetics, 37(11), 1241-1243.

Tsuchiya, H., Akiyama, T., Kuhara, T., Nakajima, Y., Ohse, M., Kurahashi, H., ... & Kobayashi, K. (2019). A case of dihydropyrimidinase deficiency incidentally detected by urine metabolome analysis. Brain and Development, 41(3), 280-284.

Hishinuma, E., Akai, F., Narita, Y., Maekawa, M., Yamaguchi, H., Mano, N., ... & Hiratsuka, M. (2017). Functional characterization of 21 allelic variants of dihydropyrimidinase. Biochemical Pharmacology, 143, 118-128.

Nakajima, Y., Meijer, J., Dobritzsch, D., Ito, T., Zhang, C., Wang, X., ... & van Kuilenburg, A. B. (2017). Dihydropyrimidinase deficiency in four East Asian patients due to novel and rare DPYS mutations affecting protein structural integrity and catalytic activity. Molecular Genetics and Metabolism, 122(4), 216-222.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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