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Mouse Anti-E2F1 Recombinant Antibody (CBFYE-0002) (V2LY-0425-LY1219)


See all E2F1 antibodies
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Published Data
Mouse Anti-E2F1 Recombinant Antibody (CBFYE-0002) (V2LY-0425-LY1219-1) in EMSAs
Mouse Anti-E2F1 Recombinant Antibody (CBFYE-0002) (V2LY-0425-LY1219-1) in EMSAs
Electrophoretic mobility shift assays (EMSAs) with biotin-labelled probes containing rs41298798-C or rs41298798-G alleles in E2F1-transfected COS1 cells. (Left) The competitor represents 200-fold excess amounts of an unlabelled probe compared with the biotin-labelled probe. (Right) EMSA using an anti-E2F1 antibody (Ab). ...View More
Funato, N., Heliövaara, A., & Boeckx, C. (2024). A regulatory variant impacting TBX1 expression contributes to basicranial morphology in Homo sapiens. The American Journal of Human Genetics, 111(5), 939-953.
Mouse Anti-E2F1 Recombinant Antibody (CBFYE-0002) (V2LY-0425-LY1219-2) in IHC
Mouse Anti-E2F1 Recombinant Antibody (CBFYE-0002) (V2LY-0425-LY1219-2) in IHC
Representative immunohistochemically stained images of fibroid xenografts from control (siNC) or MIAT knockdown treatment group (n=5), and image analysis by Halo software were shown for Ki67. ...View More
Chuang, T. D., Ton, N., Manrique, N., Rysling, S., & Khorram, O. (2024). Targeting the long non-coding RNA MIAT for the treatment of fibroids in an animal model. Clinical Science, 138(12), 699-709.
Mouse Anti-E2F1 Recombinant Antibody (CBFYE-0002) (V2LY-0425-LY1219-3) in IP
Mouse Anti-E2F1 Recombinant Antibody (CBFYE-0002) (V2LY-0425-LY1219-3) in IP
RCOR1 interacts with E2F7 but not E2F1. Co-IP showed the interaction between Flag-RCOR1 and endogenous E2F family proteins in H209 and H69 cells. ...View More
Wu, Z., Su, J., Li, F. L., Chen, T., Mayner, J., Engler, A., ... & Guan, K. L. (2023). YAP silencing by RB1 mutation is essential for small-cell lung cancer metastasis. Nature communications, 14(1), 5916.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYE-0002
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, IHC-P

Basic Information

Immunogen
Amino acids 342-386 of human E2F-1.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC-P1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
E2F Transcription Factor 1
Entrez Gene ID
Human1869
Mouse13555
Rat399489
UniProt ID
HumanQ01094
MouseQ61501
RatO09139
Research Area
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812).

Positively regulates transcription of RRP1B (PubMed:20040599).
Biological Process
Anoikis Source: Ensembl
Cellular response to fatty acid Source: Ensembl
Cellular response to hypoxia Source: Ensembl
Cellular response to nerve growth factor stimulus Source: Ensembl
Cellular response to xenobiotic stimulus Source: Ensembl
DNA damage checkpoint signaling Source: UniProtKB
Forebrain development Source: Ensembl
G1/S transition of mitotic cell cycle Source: GO_Central
Intrinsic apoptotic signaling pathway by p53 class mediator Source: Ensembl
Intrinsic apoptotic signaling pathway in response to DNA damage Source: UniProtKB
Lens fiber cell apoptotic process Source: Ensembl
mRNA stabilization Source: BHF-UCL
Negative regulation of DNA binding Source: UniProtKB
Negative regulation of fat cell differentiation Source: UniProtKB
Negative regulation of fat cell proliferation Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Negative regulation of transcription involved in G1/S transition of mitotic cell cycle Source: BHF-UCL
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of fibroblast proliferation Source: BHF-UCL
Positive regulation of gene expression Source: BHF-UCL
Positive regulation of glial cell proliferation Source: Ensembl
Positive regulation of transcription, DNA-templated Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of G1/S transition of mitotic cell cycle Source: BHF-UCL
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Spermatogenesis Source: Ensembl
Transcription, DNA-templated Source: UniProtKB
Cellular Location
Nucleus
PTM
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. Phosphorylation at Ser-364 by CHEK2 stabilizes E2F1 upon DNA damage and regulates its effect on transcription and apoptosis.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein RB1. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
More Infomation

Ma, X., Li, Y., & Zhao, B. (2022). Ribosomal protein L5 (RPL5)/E2F transcription factor 1 (E2F1) signaling suppresses breast cancer progression via regulating endoplasmic reticulum stress and autophagy. Bioengineered, 13(4), 8076-8086.

Zhou, Z. Y., Yang, J. Y., Shao, C. Z., Luo, F., & Du, W. (2022). Positive regulation of ataxia-telangiectasia-mutated protein (ATM) by E2F transcription Factor 1 (E2F-1) in cisplatin-resistant nasopharyngeal carcinoma cells. World Journal of Surgical Oncology, 20(1), 1-11.

Xie, H., Lv, S., Wang, Z., & Yuan, X. (2021). E2F transcription factor 1 elevates cyclin D1 expression by suppressing transcription of microRNA‐107 to augment progression of glioma. Brain and Behavior, 11(12), e2399.

Ye, W. Y., Lu, H. P., Li, J. D., Chen, G., He, R. Q., Wu, H. Y., ... & Dang, Y. W. (2021). Clinical Implication of E2F Transcription Factor 1 in Hepatocellular Carcinoma Tissues. Cancer Biotherapy & Radiopharmaceuticals.

Liao, R., Xie, B., Cui, J., Qi, Z., Xue, S., & Wang, Y. (2021). E2F transcription factor 1 (E2F1) promotes the transforming growth factor TGF-β1 induced human cardiac fibroblasts differentiation through promoting the transcription of CCNE2 gene. Bioengineered, 12(1), 6869-6877.

Fang, Z., Lin, M., Li, C., Liu, H., & Gong, C. (2020). A comprehensive review of the roles of E2F1 in colon cancer. American journal of cancer research, 10(3), 757.

Zheng, H., Wang, J. J., Yang, X. R., & Yu, Y. L. (2020). Upregulation of miR-34c after silencing E2F transcription factor 1 inhibits paclitaxel combined with cisplatin resistance in gastric cancer cells. World journal of gastroenterology, 26(5), 499.

Morrell, B. C., Perego, M. C., Maylem, E. R. S., Zhang, L., Schütz, L. F., & Spicer, L. J. (2020). Regulation of the transcription factor E2F1 mRNA in ovarian granulosa cells of cattle. Journal of Animal Science, 98(1), skz376.

Chun, J. N., Cho, M., Park, S., So, I., & Jeon, J. H. (2020). The conflicting role of E2F1 in prostate cancer: A matter of cell context or interpretational flexibility?. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer, 1873(1), 188336.

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For research use only. Not intended for any clinical use.

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