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Rat Anti-EBV LMP2A Recombinant Antibody (15F9) (CBMAB-V208-1556-FY)

This product is rat antibody that recognizes EBV LMP2A. The antibody 15F9 can be used for immunoassay techniques such as: WB, IHC-P.
See all EBV LMP2A antibodies
Published Data
Fig.1 LMP2A protein levels in the different groups was assessed by western blotting.
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Fig.2 Representative images (left) and quantification (right) of tube formation of NPC-EBV cells transfected with control or LMP2A-specific siRNA.
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Fig.3 LMP1 and LMP2 IF co-staining in oral epithelial cells.
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Summary

Host Animal
Rat
Specificity
EBV
Clone
15F9
Antibody Isotype
IgG1
Application
WB, IHC-P

Basic Information

Specificity
EBV
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Epstein-Barr Virus latent membrane protein 2
Introduction
Epstein-Barr virus (EBV) latent membrane protein 2 (LMP2) are two viral proteins of the Epstein-Barr virus. LMP2A/LMP2B are transmembrane proteins that act to block tyrosine kinase signaling. LMP2A is a transmembrane protein that inhibits normal B-cell signal transduction by mimicking an activated B-cell receptor (BCR). The N-terminus domain of LMP2A is tyrosine phosphorylated and associates with Src family protein tyrosine kinases (PTKs) as well as spleen tyrosine kinase (Syk). PTKs and Syk are associated with BCR signal transduction.
Alternative Names
Epstein-Barr virus latent membrane protein 2; LMP2; EBV LMP2A; EBV LMP2A; Human herpesvirus 4; HHV-4
Research Area
Isoform LMP2A maintains EBV latent infection of B-lymphocyte, by preventing lytic reactivation of the virus in response to surface immunoglobulin (sIg) cross-linking. Acts like a dominant negative inhibitor of the sIg-associated protein tyrosine kinases, LYN and SYK. Also blocks translocation of the B-cell antigen receptor (BCR) into lipid rafts, preventing the subsequent signaling and accelerated internalization of the BCR upon BCR cross-linking. Serves as a molecular scaffold to recruit SYK, LYN and E3 protein-ubiquitin ligases, such as ITCH and NEDD4L, leading to ubiquitination and potential degradation of both tyrosines kinases. Possesses a constitutive signaling activity in non-transformed cells, inducing bypass of normal B lymphocyte developmental checkpoints allowing immunoglobulin-negative cells to colonize peripheral lymphoid organs.

Isoform LMP2B may be a negative regulator of isoform LMP2A.
Biological Process
Modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Modulation by virus of host ubiquitin-protein ligase activity Source: UniProtKB-KW
Viral latency Source: InterPro
Cellular Location
Isoform LMP2A: Host cell membrane. Isoform LMP2A is localized in plasma membrane lipid rafts.
Isoform LMP2B: Host endomembrane system; Host perinuclear region. Isoform LMP2B localizes to perinuclear regions.
Topology
Cytoplasmic: 1-123
Helical: 124-144
Extracellular: 145-147
Helical: 148-168
Cytoplasmic: 169-177
Helical: 178-198
Extracellular: 199-211
Helical: 212-232
Cytoplasmic: 233-241
Helical: 242-262
Extracellular: 263-267
Helical: 268-288
Cytoplasmic: 289-296
Helical: 297-317
Extracellular: 318
Helical: 319-339
Cytoplasmic: 340-354
Helical: 355-375
Extracellular: 376-388
Helical: 389-409
Cytoplasmic: 410-422
Helical: 423-443
Extracellular: 444-449
Helical: 450-470
Cytoplasmic: 471-497
PTM
Isoform LMP2A is phosphorylated on cytoplasmic N-terminal tyrosine residues, possibly by human LYN.
Can be ubiquitinated by human ITCH and WWP2 on the N-terminus in a lysine-independent manner.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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