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Mouse Anti-ECT2 Recombinant Antibody (CBFYE-0418) (V2LY-0425-LY1365)


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Published Data
Mouse Anti-ECT2 Recombinant Antibody (CBFYE-0418) (V2LY-0425-LY1365-1) in IHC
Mouse Anti-ECT2 Recombinant Antibody (CBFYE-0418) (V2LY-0425-LY1365-1) in IHC
Endogenous Ect2 and cavin-1 in A2780 cells in 3D-CDM; MIPs of whole cell and separately captured zoomed rear region. Right: peak Ect2 staining intensity in rear, nuclear, and front regions (N=17 cells, 3 repeats). ...View More
Hetmanski, J. H., de Belly, H., Busnelli, I., Waring, T., Nair, R. V., Sokleva, V., ... & Caswell, P. T. (2019). Membrane tension orchestrates rear retraction in matrix-directed cell migration. Developmental cell, 51(4), 460-475.
Mouse Anti-ECT2 Recombinant Antibody (CBFYE-0418) (V2LY-0425-LY1365-2) in WB
Mouse Anti-ECT2 Recombinant Antibody (CBFYE-0418) (V2LY-0425-LY1365-2) in WB
Pull down of pCyk4 by GST-Ect2 (left) and gel filtration chromatography of the pCyk4-Ect2 complex (right) before and after treatment by PP2A-B'ε holoenzymes. ...View More
Wu, C. G., Chen, H., Guo, F., Yadav, V. K., Mcilwain, S. J., Rowse, M., ... & Xing, Y. (2017). PP2A-B' holoenzyme substrate recognition, regulation and role in cytokinesis. Cell discovery, 3(1), 1-19.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBFYE-0418
Antibody Isotype
IgG1, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Amino acids 584-883 of human Ect2.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
Gelatin & PBS
Preservative
Sodium Azide
Concentration
0.2 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
epithelial cell transforming sequence 2 oncogene
Entrez Gene ID
Human1894
Mouse13605
Rat361921
UniProt ID
HumanQ9H8V3
MouseQ07139
RatD3ZUD0
Research Area
Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
Biological Process
Activation of GTPase activity Source: UniProtKB
Activation of protein kinase activity Source: UniProtKB
Bicellular tight junction assembly Source: UniProtKB
Cell differentiation Source: UniProtKB-KW
Cell morphogenesis Source: Ensembl
Cellular response to calcium ion Source: UniProtKB
Cellular response to hydrogen peroxide Source: UniProtKB
Cellular response to ionizing radiation Source: UniProtKB
Intracellular signal transduction Source: InterPro
Mitotic cytokinesis Source: UniProtKB
Nervous system development Source: GO_Central
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of cytokinesis Source: UniProtKB
Positive regulation of GTPase activity Source: UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Positive regulation of neuron differentiation Source: UniProtKB
Positive regulation of protein import into nucleus Source: UniProtKB
Protein homooligomerization Source: UniProtKB
Protein transport Source: UniProtKB-KW
Regulation of attachment of spindle microtubules to kinetochore Source: UniProtKB
Regulation of cytokinesis, actomyosin contractile ring assembly Source: InterPro
Regulation of protein kinase activity Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Cellular Location
Cytoplasm; Nucleus; Spindle; Cleavage furrow; Midbody; Cell junction; Tight junction. Sequestered within the nucleus during interphase. Dispersed throughout the cytoplasm upon breakdown of the nuclear envelope during mitosis. Colocalizes with the centralspindlin complex to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalized with RhoA at the midbody. Its subcellular localization to tight junction is increased by calcium. Localized predominantly in the cytoplasm of numerous carcinoma cells.
PTM
Phosphorylated by PLK1 in vitro. Hyperphosphorylated during the G2 phase of the cell cycle. Phosphorylation at Thr-373 oCcurs during the G2/M phase, relieves its auto-inhibition status and stimulates its GEF activity. Phosphorylation at Thr-444 in G2/M phase is required for subsequent binding with PLK1 and Rho exchange activation. Dephosphorylated at the time of cytokinesis. Phosphorylation at Thr-359 is required for its transformation activity in cancer cells.
More Infomation

Mamoor, S. (2021). Over-expression of epithelial cell transforming 2 in human endometrial cancer.

Kosibaty, Z., Murata, Y., Minami, Y., Noguchi, M., & Sakamoto, N. (2021). ECT2 promotes lung adenocarcinoma progression through extracellular matrix dynamics and focal adhesion signaling. Cancer science, 112(2), 703-714.

Cao, C., Han, P., Liu, L., Tang, Y., Tian, S., Zhang, K., ... & Gong, W. (2021). Epithelial cell transforming factor ECT2 is an important regulator of DNA double-strand break repair and genome stability. Journal of Biological Chemistry, 297(3).

Li, C., Peng, Z., Wang, Y., Lam, G., Nissen, N., Tang, J., ... & Wang, Q. (2021). Epithelial cell transforming 2 is regulated by Yes-associated protein 1 and mediates pancreatic cancer progression and metastasis. American Journal of Physiology-Gastrointestinal and Liver Physiology.

Chen, M., Pan, H., Sun, L., Shi, P., Zhang, Y., Li, L., ... & Chen, Z. (2020). Structure and regulation of human epithelial cell transforming 2 protein. Proceedings of the National Academy of Sciences, 117(2), 1027-1035.

Kosibaty, Z., Murata, Y., Minami, Y., Dai, T., Kano, J., Matsuoka, R., ... & Noguchi, M. (2019). Cytoplasmic expression of epithelial cell transforming sequence 2 in lung adenocarcinoma and its implications for malignant progression. Laboratory Investigation, 99(4), 551-567.

Wang, X., Tong, Z., & Liu, H. (2019). MiR-223-3p targeting epithelial cell transforming sequence 2 oncogene inhibits the activity, apoptosis, invasion and migration of MDA-MB-468 breast cancer cells. OncoTargets and therapy, 12, 7675.

Li, B., Lin, J. P., Li, Z., Yin, C., Yang, J. B., & Meng, Y. Q. (2019). Clinicopathological and prognostic significance of epithelial cell transforming sequence 2 expression in cancers: a systematic review and meta-analysis. Artificial Cells, Nanomedicine, and Biotechnology, 47(1), 4139-4148.

Ulke, H. M., Mutze, K., Lehmann, M., Wagner, D. E., Heinzelmann, K., Günther, A., ... & Königshoff, M. (2019). The oncogene ECT2 contributes to a hyperplastic, proliferative lung epithelial cell phenotype in idiopathic pulmonary fibrosis. American Journal of Respiratory Cell and Molecular Biology, 61(6), 713-726.

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For research use only. Not intended for any clinical use.

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