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Mouse Anti-ECT2 Recombinant Antibody (5D4) (CBMAB-A2433-LY)

The product is antibody recognizes ECT2. The antibody 5D4 immunoassay techniques such as: WB, ELISA.
See all ECT2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
5D4
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
ECT2 (AAH06838.1, 46 a.a. ~ 145 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
epithelial cell transforming sequence 2 oncogene
Introduction
The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. [provided by RefSeq]
Entrez Gene ID
1894
UniProt ID
Q9H8V3
Alternative Names
FLJ10461; MGC138291
Research Area
Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
Biological Process
Activation of GTPase activity Source: UniProtKB
Activation of protein kinase activity Source: UniProtKB
Bicellular tight junction assembly Source: UniProtKB
Cell differentiation Source: UniProtKB-KW
Cell morphogenesis Source: Ensembl
Cellular response to calcium ion Source: UniProtKB
Cellular response to hydrogen peroxide Source: UniProtKB
Cellular response to ionizing radiation Source: UniProtKB
Intracellular signal transduction Source: InterPro
Mitotic cytokinesis Source: UniProtKB
Nervous system development Source: GO_Central
Positive regulation of apoptotic process Source: UniProtKB
Positive regulation of cytokinesis Source: UniProtKB
Positive regulation of GTPase activity Source: UniProtKB
Positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
Positive regulation of neuron differentiation Source: UniProtKB
Positive regulation of protein import into nucleus Source: UniProtKB
Protein homooligomerization Source: UniProtKB
Protein transport Source: UniProtKB-KW
Regulation of attachment of spindle microtubules to kinetochore Source: UniProtKB
Regulation of cytokinesis, actomyosin contractile ring assembly Source: InterPro
Regulation of protein kinase activity Source: UniProtKB
Regulation of small GTPase mediated signal transduction Source: Reactome
Cellular Location
Cytoplasm; Nucleus; Spindle; Cleavage furrow; Midbody; Cell junction; Tight junction. Sequestered within the nucleus during interphase. Dispersed throughout the cytoplasm upon breakdown of the nuclear envelope during mitosis. Colocalizes with the centralspindlin complex to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalized with RhoA at the midbody. Its subcellular localization to tight junction is increased by calcium. Localized predominantly in the cytoplasm of numerous carcinoma cells.
PTM
Phosphorylated by PLK1 in vitro. Hyperphosphorylated during the G2 phase of the cell cycle. Phosphorylation at Thr-373 oCcurs during the G2/M phase, relieves its auto-inhibition status and stimulates its GEF activity. Phosphorylation at Thr-444 in G2/M phase is required for subsequent binding with PLK1 and Rho exchange activation. Dephosphorylated at the time of cytokinesis. Phosphorylation at Thr-359 is required for its transformation activity in cancer cells.

Mamoor, S. (2021). Over-expression of epithelial cell transforming 2 in human endometrial cancer.

Kosibaty, Z., Murata, Y., Minami, Y., Noguchi, M., & Sakamoto, N. (2021). ECT2 promotes lung adenocarcinoma progression through extracellular matrix dynamics and focal adhesion signaling. Cancer science, 112(2), 703-714.

Cao, C., Han, P., Liu, L., Tang, Y., Tian, S., Zhang, K., ... & Gong, W. (2021). Epithelial cell transforming factor ECT2 is an important regulator of DNA double-strand break repair and genome stability. Journal of Biological Chemistry, 297(3).

Li, C., Peng, Z., Wang, Y., Lam, G., Nissen, N., Tang, J., ... & Wang, Q. (2021). Epithelial cell transforming 2 is regulated by Yes-associated protein 1 and mediates pancreatic cancer progression and metastasis. American Journal of Physiology-Gastrointestinal and Liver Physiology.

Chen, M., Pan, H., Sun, L., Shi, P., Zhang, Y., Li, L., ... & Chen, Z. (2020). Structure and regulation of human epithelial cell transforming 2 protein. Proceedings of the National Academy of Sciences, 117(2), 1027-1035.

Kosibaty, Z., Murata, Y., Minami, Y., Dai, T., Kano, J., Matsuoka, R., ... & Noguchi, M. (2019). Cytoplasmic expression of epithelial cell transforming sequence 2 in lung adenocarcinoma and its implications for malignant progression. Laboratory Investigation, 99(4), 551-567.

Wang, X., Tong, Z., & Liu, H. (2019). MiR-223-3p targeting epithelial cell transforming sequence 2 oncogene inhibits the activity, apoptosis, invasion and migration of MDA-MB-468 breast cancer cells. OncoTargets and therapy, 12, 7675.

Li, B., Lin, J. P., Li, Z., Yin, C., Yang, J. B., & Meng, Y. Q. (2019). Clinicopathological and prognostic significance of epithelial cell transforming sequence 2 expression in cancers: a systematic review and meta-analysis. Artificial Cells, Nanomedicine, and Biotechnology, 47(1), 4139-4148.

Ulke, H. M., Mutze, K., Lehmann, M., Wagner, D. E., Heinzelmann, K., Günther, A., ... & Königshoff, M. (2019). The oncogene ECT2 contributes to a hyperplastic, proliferative lung epithelial cell phenotype in idiopathic pulmonary fibrosis. American Journal of Respiratory Cell and Molecular Biology, 61(6), 713-726.

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For research use only. Not intended for any clinical use.

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