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Rabbit Anti-Egln2 Recombinant Antibody (CBYC-P057) (CBMAB-P0294-YC)

Provided herein is a Rabbit monoclonal antibody against Human Egl-9 Family Hypoxia Inducible Factor 2. The antibody can be used for immunoassay techniques, such as WB, IHC-P, IF, ICC, FC.
See all Egln2 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
CBYC-P057
Antibody Isotype
IgG
Application
WB, IHC-P, IF, ICC, FC

Basic Information

Immunogen
Recombinant protein
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid in TBS, pH 7.4, 1% BSA, 40% glycerol
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Egl-9 Family Hypoxia Inducible Factor 2
Introduction
The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.
Entrez Gene ID
Human112398
Mouse112406
Rat308457
UniProt ID
HumanQ96KS0
MouseQ91YE2
RatQ6AYU4
Alternative Names
Egl-9 Family Hypoxia Inducible Factor 2; Prolyl Hydroxylase Domain-Containing Protein 1; Hypoxia-Inducible Factor Prolyl Hydroxylase 1; HIF-Prolyl Hydroxylase 1; Estrogen-Induced Tag 6; HIF-PH1; HPH-1; HPH-3; EIT6;
Research Area
Prolyl hydroxylase that mediates hydroxylation of proline residues in target proteins, such as ATF4, IKBKB, CEP192 and HIF1A (PubMed:11595184, PubMed:12039559, PubMed:15925519, PubMed:16509823, PubMed:17114296, PubMed:23932902).

Target proteins are preferentially recognized via a LXXLAP motif (PubMed:11595184, PubMed:12039559, PubMed:15925519).

Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins (PubMed:11595184, PubMed:12039559, PubMed:12181324, PubMed:15925519, PubMed:19339211).

Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A (PubMed:11595184, PubMed:12039559, PubMed:12181324, PubMed:15925519).

Also hydroxylates HIF2A (PubMed:11595184, PubMed:12039559, PubMed:15925519).

Has a preference for the CODD site for both HIF1A and HIF2A (PubMed:11595184, PubMed:12039559, PubMed:15925519).

Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex (PubMed:11595184, PubMed:12039559, PubMed:15925519).

Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes (PubMed:11595184, PubMed:12039559, PubMed:15925519).

EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle (PubMed:11595184, PubMed:12039559, PubMed:15925519).

Also regulates susceptibility to normoxic oxidative neuronal death (PubMed:11595184, PubMed:12039559, PubMed:15925519).

Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation (PubMed:23932902).

Hydroxylates IKBKB, mediating NF-kappa-B activation in hypoxic conditions (PubMed:17114296).

Also mediates hydroxylation of ATF4, leading to decreased protein stability of ATF4 (By similarity).
Biological Process
Cell redox homeostasis Source: UniProtKB
Cellular response to hypoxia Source: GO_Central
Intracellular estrogen receptor signaling pathway Source: UniProtKB
Peptidyl-proline hydroxylation to 4-hydroxy-L-proline Source: UniProtKB
Positive regulation of protein catabolic process Source: UniProtKB
Regulation of cell growth Source: UniProtKB
Regulation of neuron apoptotic process Source: UniProtKB
Response to hypoxia Source: UniProtKB
Cellular Location
Nucleus
PTM
Ubiquitinated by SIAH1 and/or SIAH2 in response to the unfolded protein response (UPR), leading to its degradation.

Li, S., Fu, J., Wang, Y., Hu, C., & Xu, F. (2021). LncRNA MIAT enhances cerebral ischaemia/reperfusion injury in rat model via interacting with EGLN2 and reduces its ubiquitin‐mediated degradation. Journal of cellular and molecular medicine, 25(21), 10140-10151.

Cheng, L., Xing, Z., Zhang, P., & Xu, W. (2020). Long non-coding RNA LINC00662 promotes proliferation and migration of breast cancer cells via regulating the miR-497-5p/EglN2 axis. Acta Biochimica Polonica, 67(2), 229-237.

Rahimi, N., Azizi, M., Bahari, G., Narouie, B., & Hashemi, M. (2020). Association of EGLN2 rs10680577 Polymorphism with the Risk and Clinicopathological Features of Patients with Prostate Cancer. Asian Pacific journal of cancer prevention: APJCP, 21(5), 1221.

Chen, Y., Cao, K., Li, J., Wang, A., Sun, L., Tang, J., ... & Liu, Y. (2019). Overexpression of long non‐coding RNA NORAD promotes invasion and migration in malignant melanoma via regulating the MIR‐205‐EGLN2 pathway. Cancer medicine, 8(4), 1744-1754.

Zhang, R., Lai, L., He, J., Chen, C., You, D., Duan, W., ... & Christiani, D. C. (2019). EGLN2 DNA methylation and expression interact with HIF1A to affect survival of early-stage NSCLC. Epigenetics, 14(2), 118-129.

Zhang, S., Zhu, K., Zhang, Z., Wang, H., & Wang, X. (2019). Association between an indel polymorphism within the distal promoter of EGLN2 and cancer risk: An updated meta‐analysis. Molecular genetics & genomic medicine, 7(10), e00936.

Hashemi, M., Tabasi, F., & Ansari, H. (2018). 4-bp insertion/deletion polymorphism within the promoter of EGLN2 gene is associated with susceptibility to cancer in Asian population: Evidence from a meta-analysis. Meta Gene, 17, 141-146.

Hashemi, M., Danesh, H., Bizhani, F., Sattarifard, H., Hashemi, S. M., & Bahari, G. (2018). Detection of a 4-bp insertion/deletion polymorphism within the promoter of EGLN2 using mismatch PCR-RFLP and its association with susceptibility to breast cancer. Asian Pacific Journal of Cancer Prevention: APJCP, 19(4), 923.

Xiang, J., Hu, Q., Qin, Y., Ji, S., Xu, W., Liu, W., ... & Yu, X. (2018). TCF7L2 positively regulates aerobic glycolysis via the EGLN2/HIF-1α axis and indicates prognosis in pancreatic cancer. Cell death & disease, 9(3), 1-14.

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For research use only. Not intended for any clinical use.

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