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Mouse Anti-EHMT2 Antibody (3D9) (CBMAB-0335-YC)

Provided herein are mouse monoclonal antibodies against Human EHMT2. The antibody clone 3D9 can be used for immunoassay techniques, such as IP and MA.
See all EHMT2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3D9
Antibody Isotype
IgG2b
Application
IP, MA

Basic Information

Immunogen
Recombinant protein
Specificity
Human
Antibody Isotype
IgG2b
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
EHMT2
Introduction
EHMT2 (euchromatic histone lysine methyltransferase 2) is a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus.
Entrez Gene ID
10919
UniProt ID
Q96KQ7
Alternative Names
Euchromatic Histone-Lysine N-Methyltransferase 2; Histone H3-K9 Methyltransferase 3; HLA-B Associated Transcript 8; Lysine N-Methyltransferase 1C; H3-K9-HMTase 3; C6orf30; KMT1C; BAT8; NG36; G9A; Histone-Lysine N-Methyltransferase, H3 Lysine-9 Specific 3; Chromosome 6 Open Reading Frame 30; Ankyrin Repeat-Containing Protein; G9A Histone Methyltransferase; HLA-B-Associated Transcript 8; Protein G9a; EC 2.1.1.43; EC 2.1.1.-; GAT8
Research Area
Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
Biological Process
Behavioral response to cocaine Source: Ensembl
Cellular response to cocaine Source: Ensembl
Cellular response to drug Source: Ensembl
Cellular response to starvation Source: MGI
DNA methylation Source: UniProtKB
DNA methylation on cytosine within a CG sequence Source: Ensembl
Fertilization Source: Ensembl
Histone lysine methylation Source: MGI
Histone methylation Source: UniProtKB
Long-term memory Source: Ensembl
Negative regulation of autophagosome assembly Source: Ensembl
Negative regulation of transcription by RNA polymerase II Source: MGI
Neuron fate specification Source: Ensembl
Organ growth Source: Ensembl
Peptidyl-lysine dimethylation Source: UniProtKB
Phenotypic switching Source: Ensembl
Regulation of DNA methylation Source: Ensembl
Regulation of DNA replication Source: UniProtKB
Regulation of histone H3-K4 methylation Source: Ensembl
Regulation of histone H3-K9 methylation Source: Ensembl
Response to ethanol Source: Ensembl
Response to fungicide Source: Ensembl
Spermatid development Source: Ensembl
Synaptonemal complex assembly Source: Ensembl
Cellular Location
Nucleus; Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin.
PTM
Methylated at Lys-185; automethylated.

Zhang, J., Chen, W., Ma, W., Song, K., Lee, S., Han, C., & Wu, T. (2022). Epigenetic Silencing of 15-Hydroxyprostaglandin Dehydrogenase by Histone Methyltransferase EHMT2/G9a in Cholangiocarcinoma. Molecular Cancer Research, 20(3), 350-360.

Nachiyappan, A., Gupta, N., & Taneja, R. (2022). EHMT1/EHMT2 in EMT, cancer stemness and drug resistance: emerging evidence and mechanisms. The FEBS Journal, 289(5), 1329-1351.

Ryu, T. Y., Kim, K., Han, T. S., Lee, M. O., Lee, J., Choi, J., ... & Cho, H. S. (2022). Human gut-microbiome-derived propionate coordinates proteasomal degradation via HECTD2 upregulation to target EHMT2 in colorectal cancer. The ISME journal, 16(5), 1205-1221.

Daemen, A., Mr, A. N. L., Wang, X., Hafner, M., Poon, C., Modrusan, Z., ... & Junttila, M. R. (2022). EHMT2 methyltransferase governs cell identity in the lung and is required for KRASG12D tumor development and propagation. Elife, 11, e57648.

Jan, S., Dar, M. I., Wani, R., Sandey, J., Mushtaq, I., Lateef, S., & Syed, S. H. (2021). Targeting EHMT2/G9a for cancer therapy: Progress and perspective. European Journal of Pharmacology, 893, 173827.

Souza, B. K., Freire, N. H., Jaeger, M., de Farias, C. B., Brunetto, A. L., Brunetto, A. T., & Roesler, R. (2021). EHMT2/G9a as an epigenetic target in pediatric and adult brain tumors. International Journal of Molecular Sciences, 22(20), 11292.

Pan, Z., Du, S., Wang, K., Guo, X., Mao, Q., Feng, X., ... & Tao, Y. X. (2021). Downregulation of a dorsal root ganglion‐specifically enriched long noncoding RNA is required for neuropathic pain by negatively regulating RALY‐triggered Ehmt2 expression. Advanced Science, 8(13), 2004515.

Lee, R. A., Tsay, A., Chang, M., Li, D., & Wang, J. C. (2020). OR14-03 The Transcriptional Coactivation Function of EHMT2 Restricts Chronic Glucocorticoid Exposure Induced Insulin Resistance. Journal of the Endocrine Society, 4(Supplement_1), OR14-03.

Watson, Z. L., Yamamoto, T. M., McMellen, A., Kim, H., Hughes, C. J., Wheeler, L. J., ... & Bitler, B. G. (2019). Histone methyltransferases EHMT1 and EHMT2 (GLP/G9A) maintain PARP inhibitor resistance in high-grade serous ovarian carcinoma. Clinical epigenetics, 11(1), 1-16.

Chen, G., Yu, X., Zhang, M., Zheng, A., Wang, Z., Zuo, Y., ... & Liao, S. (2019). Inhibition of euchromatic histone lysine methyltransferase 2 (EHMT2) suppresses the proliferation and invasion of cervical cancer cells. Cytogenetic and genome research, 158(4), 205-212.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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