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Mouse Anti-EIF4E2 Recombinant Antibody (1A11) (CBMAB-A2535-LY)

The product is antibody recognizes EIF4E2. The antibody 1A11 immunoassay techniques such as: WB, ELISA.
See all EIF4E2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
1A11
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
EIF4E2 (AAH05392, 1 a.a. ~ 245 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
eukaryotic translation initiation factor 4E family member 2
Entrez Gene ID
9470
UniProt ID
O60573
Alternative Names
4E-LP; 4EHP; EIF4EL3; IF4e
Research Area
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation (PubMed:17368478, PubMed:25624349, PubMed:9582349).

Acts as a repressor of translation initiation (PubMed:22751931).

In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap (By similarity).

In P-bodies, component of a complex that promotes miRNA-mediated translational repression (PubMed:28487484).
Biological Process
Gene silencing by RNA Source: UniProtKB-KW
Negative regulation of translation Source: MGI
Positive regulation of miRNA mediated inhibition of translation Source: UniProtKB
Cellular Location
Cytoplasm; P-body
PTM
Ubiquitinated by ARIH1 (PubMed:14623119, PubMed:25624349). The consequences of ubiquitination are however unclear: according to a report, EIF4E2 ubiquitination leads to promote EIF4E2 cap-binding and protein translation arrest (PubMed:25624349). According to another report ubiquitination leads to its subsequent degradation (PubMed:14623119).
ISGylation enhances its cap structure-binding activity and translation-inhibition activity.

Sun, L., Yang, H., He, D., Chen, J., Dong, Z., Luo, S., ... & Zhang, M. (2022). Mammalian eIF4E2-GSK3β maintains basal phosphorylation of p53 to resist senescence under hypoxia. Cell death & disease, 13(5), 1-13.

Kuroiwa, K., Thenault, C., Nogué, F., Perrot, L., Mazier, M., & Gallois, J. L. (2022). CRISPR-based knock-out of eIF4E2 in a cherry tomato background successfully recapitulates resistance to pepper veinal mottle virus. Plant Science, 316, 111160.

Falk, F., Palhares, R. M., Waithaka, A., & Clayton, C. (2022). Roles and interactions of the specialized initiation factors EIF4E2, EIF4E5 and EIF4E6 in Trypanosoma brucei: EIF4E2 maintains the abundances of S-phase mRNAs. bioRxiv.

Zinshteyn, B., Sinha, N. K., Enam, S. U., Koleske, B., & Green, R. (2021). Translational repression of NMD targets by GIGYF2 and EIF4E2. PLoS genetics, 17(10), e1009813.

Yang, B., Gu, A., & Wu, Y. (2021). High EIF4E2 expression is an independent prognostic risk factor for poor overall survival and recurrence-free survival in uveal melanoma. Experimental Eye Research, 206, 108558.

Moury, B., Lebaron, C., Szadkowski, M., Khalifa, M. B., Girardot, G., Bi, B. A. B., ... & Gallois, J. L. (2020). Knock-out mutation of eukaryotic initiation factor 4E2 (eIF4E2) confers resistance to pepper veinal mottle virus in tomato. Virology, 539, 11-17.

Kelly, N. J., Varga, J. F. A., Specker, E. J., Romeo, C. M., Coomber, B. L., & Uniacke, J. (2018). Hypoxia activates cadherin-22 synthesis via eIF4E2 to drive cancer cell migration, invasion and adhesion. Oncogene, 37(5), 651-662.

Freire, E. R., Moura, D., Bezerra, M. J., Xavier, C. C., Morais-Sobral, M. C., Vashisht, A. A., ... & Campbell, D. A. (2018). Trypanosoma brucei EIF4E2 cap-binding protein binds a homolog of the histone-mRNA stem-loop-binding protein. Current genetics, 64(4), 821-839.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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