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Mouse Anti-EIF5A2 (AA 66-153) Recombinant Antibody (CBFYE-0751) (CBMAB-E1191-FY)

This product is mouse antibody that recognizes EIF5A2. The antibody CBFYE-0751 can be used for immunoassay techniques such as: ELISA, WB.
See all EIF5A2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYE-0751
Antibody Isotype
IgG1, κ
Application
ELISA, WB

Basic Information

Immunogen
EIF5A2 partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: GKKYEDICPS THNMDVPNIK RNDYQLICIQ DGYLSLLTET GEVREDLKLP EGELGKEIEG KYNAGEDVQV SVMCAMSEEY AVAIKPCK
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 66-153

Target

Full Name
Eukaryotic Translation Initiation Factor 5A2
Introduction
EIF5A2 (Eukaryotic Translation Initiation Factor 5A2) is a Protein Coding gene. Among its related pathways are Metabolism of proteins and Gamma carboxylation, hypusine formation and arylsulfatase activation. Gene Ontology (GO) annotations related to this gene include RNA binding and translation elongation factor activity. An important paralog of this gene is EIF5A.
Entrez Gene ID
56648
UniProt ID
Q9GZV4
Alternative Names
Eukaryotic Translation Initiation Factor 5A2; EIF-5A-2; EIF-5A2; Eukaryotic Translation Initiation Factor 5A-2; Eukaryotic Initiation Factor 5A Isoform 2; Eukaryotic Initiation Factor 5A; EIF5AII
Research Area
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity).
Biological Process
mRNA transport Source: UniProtKB-KW
Polyamine homeostasis Source: UniProtKB
Positive regulation of translational elongation Source: GO_Central
Positive regulation of translational termination Source: InterPro
Protein transport Source: UniProtKB-KW
Spermatogenesis Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane; Nucleus; Nuclear pore complex; Cytoplasm. Hypusine modification promotes the nuclear export and cytoplasmic localization and there was a dynamic shift in the localization from predominantly cytoplasmic to primarily nuclear under apoptotic inducing conditions.
PTM
eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).

Zhang, Z., He, G., Lv, Y., Liu, Y., Niu, Z., Feng, Q., ... & Xu, J. (2022). HERC3 regulates epithelial-mesenchymal transition by directly ubiquitination degradation EIF5A2 and inhibits metastasis of colorectal cancer. Cell death & disease, 13(1), 1-12.

Zhao, G., Zhang, W., Dong, P., Watari, H., Guo, Y., Pfeffer, L. M., ... & Yue, J. (2021). EIF5A2 controls ovarian tumor growth and metastasis by promoting epithelial to mesenchymal transition via the TGFβ pathway. Cell & bioscience, 11(1), 1-12.

Zheng, Y., Li, P., Huang, H., Ye, X., Chen, W., Xu, G., & Zhang, F. (2021). Androgen receptor regulates eIF5A2 expression and promotes prostate cancer metastasis via EMT. Cell death discovery, 7(1), 1-8.

Luan, W., Ding, Y., Yuan, H., Ma, S., Ruan, H., Wang, J., ... & Bu, X. (2020). Long non-coding RNA LINC00520 promotes the proliferation and metastasis of malignant melanoma by inducing the miR-125b-5p/EIF5A2 axis. Journal of Experimental & Clinical Cancer Research, 39(1), 1-16.

Lin, Y. M., Chen, M. L., Chen, C. L., Yeh, C. M., & Sung, W. W. (2020). Overexpression of EIF5A2 predicts poor prognosis in patients with oral squamous cell carcinoma. Diagnostics, 10(7), 436.

Liu, Y., Lei, P., Qiao, H., Sun, K., Lu, X., Bao, F., ... & Xue, F. (2019). miR-9 enhances the chemosensitivity of AML cells to daunorubicin by targeting the EIF5A2/MCL-1 axis. International journal of biological sciences, 15(3), 579.

Guan, X., Gu, S., Yuan, M., Zheng, X., & Wu, J. (2019). MicroRNA‑33a‑5p overexpression sensitizes triple‑negative breast cancer to doxorubicin by inhibiting eIF5A2 and epithelial‑mesenchymal transition. Oncology Letters, 18(6), 5986-5994.

Zheng, X., Gao, L., Wang, B. T., Shen, P., Yuan, X. F., Zhang, L. Q., ... & Wang, X. M. (2019). Overexpression of EIF5A2 is associated with poor survival and aggressive tumor biology in gallbladder cancer. Histology and Histopathology, 35(6), 579-587.

Yang, Y., Cui, H., & Wang, X. (2019). Downregulation of EIF5A2 by miR-221-3p inhibits cell proliferation, promotes cell cycle arrest and apoptosis in medulloblastoma cells. Bioscience, Biotechnology, and Biochemistry, 83(3), 400-408.

Sun, J., Xu, Z., Lv, H., Wang, Y., Wang, L., Ni, Y., ... & Cheng, X. (2018). eIF5A2 regulates the resistance of gastric cancer cells to cisplatin via induction of EMT. American journal of translational research, 10(12), 4269.

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For research use only. Not intended for any clinical use.

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