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Mouse Anti-EIF6 Recombinant Antibody (2I11) (CBMAB-BR166LY)

The product is antibody recognizes EIF6. The antibody 2I11 immunoassay techniques such as: FC, IHC-P, WB.
See all EIF6 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
2I11
Antibody Isotype
IgG2a
Application
FC: 1-3 μg/1x10 cells, IHC-P: 0.5-1 μg/ml, WB: 0.1-0.5 μg/ml

Basic Information

Immunogen
E.coli-derived human EIF6 recombinant protein (Position: N66-T210)
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Preservative
0.05 mg sodium azide
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
eukaryotic translation initiation factor 6
Introduction
Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
Entrez Gene ID
Human3692
Mouse22384
Rat305506
UniProt ID
HumanP56537
MouseO55135
RatQ3KRD8
Alternative Names
Eukaryotic translation initiation factor 6; eIF-6; B(2)GCN homolog; B4 integrin interactor; CAB; p27(BBP); EIF6; EIF3A; ITGB4BP; OK/SW-cl.27
Research Area
Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity (PubMed:10085284, PubMed:14654845, PubMed:21536732).

In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis (By similarity).

Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (PubMed:17507929).

Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth (By similarity).
Biological Process
Assembly of large subunit precursor of preribosome Source: GO_Central
Gene silencing by miRNA Source: UniProtKB
Maturation of 5.8S rRNA Source: GO_Central
Maturation of LSU-rRNA Source: GO_Central
Mature ribosome assembly Source: UniProtKB
miRNA mediated inhibition of translation Source: UniProtKB
Positive regulation of translation Source: UniProtKB
Regulation of fatty acid biosynthetic process Source: UniProtKB
Regulation of glycolytic process Source: UniProtKB
Regulation of megakaryocyte differentiation Source: UniProtKB
Regulation of reactive oxygen species metabolic process Source: UniProtKB
Response to insulin Source: UniProtKB
Ribosomal subunit export from nucleus Source: GO_Central
Cellular Location
Cytoplasm; Nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.
PTM
Phosphorylation at Ser-174 and Ser-175 by CSNK1D/CK1 promotes nuclear export.
Ufmylated by UFL1.

Li, Y., Wang, Z., Su, P., Liang, Y., Li, Z., Zhang, H., ... & Yang, Q. (2022). circ-EIF6 encodes EIF6-224aa to promote TNBC progression via stabilizing MYH9 and activating the Wnt/beta-catenin pathway. Molecular Therapy, 30(1), 415-430.

Jaako, P., Faille, A., Tan, S., Wong, C. C., Escudero-Urquijo, N., Castro-Hartmann, P., ... & Warren, A. J. (2022). eIF6 rebinding dynamically couples ribosome maturation and translation. Nature communications, 13(1), 1-11.

Scagliola, A., Miluzio, A., Ventura, G., Oliveto, S., Cordiglieri, C., Manfrini, N., ... & Biffo, S. (2021). Targeting of eIF6-driven translation induces a metabolic rewiring that reduces NAFLD and the consequent evolution to hepatocellular carcinoma. Nature Communications, 12(1), 1-16.

Sun, L., Liu, S., Wang, X., Zheng, X., Chen, Y., & Shen, H. (2021). eIF6 promotes the malignant progression of human hepatocellular carcinoma via the mTOR signaling pathway. Journal of translational medicine, 19(1), 1-17.

Koh, A. L., Bonnard, C., Lim, J. Y., Liew, W. K., Thoon, K. C., Thomas, T., ... & Jamuar, S. S. (2020). Heterozygous missense variant in EIF6 gene: A novel form of Shwachman–Diamond syndrome?. American Journal of Medical Genetics Part A, 182(9), 2010-2020.

Golob-Schwarzl, N., Puchas, P., Gogg-Kamerer, M., Weichert, W., Göppert, B., & Haybaeck, J. (2020). New pancreatic cancer biomarkers eIF1, eIF2D, eIF3C and eIF6 play a major role in translational control in ductal adenocarcinoma. Anticancer Research, 40(6), 3109-3118.

Ye, C., Liu, B., Lu, H., Liu, J., Rabson, A. B., Jacinto, E., ... & Shen, Z. (2020). BCCIP is required for nucleolar recruitment of eIF6 and 12S pre-rRNA production during 60S ribosome biogenesis. Nucleic acids research, 48(22), 12817-12832.

Tan, S., Kermasson, L., Hoslin, A., Jaako, P., Faille, A., Acevedo-Arozena, A., ... & Revy, P. (2019). EFL1 mutations impair eIF6 release to cause Shwachman-Diamond syndrome. Blood, The Journal of the American Society of Hematology, 134(3), 277-290.

Lin, J., Yu, X., Xie, L., Wang, P., Li, T., Xiao, Y., ... & Wang, J. (2019). Eif6 promotes colorectal cancer proliferation and invasion by regulating akt-related signaling pathways. Journal of Biomedical Nanotechnology, 15(7), 1556-1567.

Liu, F., Zhang, J., Qin, L., Yang, Z., Xiong, J., Zhang, Y., ... & Liu, J. (2018). Circular RNA EIF6 (Hsa_circ_0060060) sponges miR-144-3p to promote the cisplatin-resistance of human thyroid carcinoma cells by autophagy regulation. Aging (Albany NY), 10(12), 3806.

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For research use only. Not intended for any clinical use.

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