Mouse Anti-ELOF1 (AA 1-83) Recombinant Antibody (CBFYE-0801) (CBMAB-E1249-FY)

This product is mouse antibody that recognizes ELOF1. The antibody CBFYE-0801 can be used for immunoassay techniques such as: ELISA, WB.
See all ELOF1 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBFYE-0801

Antibody Isotype

IgG2a, κ

Application

ELISA, WB

Basic Information

Immunogen

ELOF1 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: MGRRKSKRKP PPKKKMTGTL ETQFTCPFCN HEKSCDVKMD RARNTGVISC TVCLEEFQTP ITYLSEPVDV YSDWIDACEA ANQ

Specificity

Human

Antibody Isotype

IgG2a, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 1-83

Target

Full Name

elongation factor 1 homolog (S. cerevisiae)

Introduction

ELOF1 (Elongation Factor 1 Homolog) is a Protein Coding gene. Gene Ontology (GO) annotations related to this gene include translation elongation factor activity.

Entrez Gene ID

84337

UniProt ID

P60002

Alternative Names

Elongation Factor 1 Homolog; Elongation Factor 1 Homolog (ELF1, S. Cerevisiae); Elongation Factor 1 Homolog (S. Cerevisiae); Transcription Elongation Factor 1 Homolog; ELF1 Homolog, Elongation Factor 1; ELF1

Research Area

Transcription elongation factor implicated in the maintenance of proper chromatin structure in actively transcribed regions.

Biological Process

Chromatin-mediated maintenance of transcription Source: GO_Central
Transcription elongation from RNA polymerase II promoter Source: GO_Central

Cellular Location

Nucleus

References

Carnie, C. J., & Jackson, S. P. (2021). The ELOF (1) ant in the room of TCR. Nature Cell Biology, 23(6), 584-586.

Geijer, M. E., Zhou, D., Selvam, K., Steurer, B., Mukherjee, C., Evers, B., ... & Marteijn, J. A. (2021). Elongation factor ELOF1 drives transcription-coupled repair and prevents genome instability. Nature cell biology, 23(6), 608-619.

van der Weegen, Y., de Lint, K., van den Heuvel, D., Nakazawa, Y., Mevissen, T. E., van Schie, J. J., ... & Luijsterburg, M. S. (2021). ELOF1 is a transcription-coupled DNA repair factor that directs RNA polymerase II ubiquitylation. Nature Cell Biology, 23(6), 595-607.

Geijer, M. E., Zhou, D., Selvam, K., Steurer, B., Mukherjee, C., Evers, B., ... & Marteijn, J. A. (2021). Publisher Correction: Elongation factor ELOF1 drives transcription-coupled repair and prevents genome instability. Nature Cell Biology, 23(7), 809-809.

Tellier, A. P., Archambault, D., Tremblay, K. D., & Mager, J. (2019). The elongation factor Elof1 is required for mammalian gastrulation. PloS one, 14(7), e0219410.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Mouse Anti-ELOF1 Recombinant Antibody (4F6)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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