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Mouse Anti-ELP3 Recombinant Antibody (CBFYE-0807) (CBMAB-E1255-FY)

This product is mouse antibody that recognizes ELP3. The antibody CBFYE-0807 can be used for immunoassay techniques such as: WB, IF, IP.
See all ELP3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYE-0807
Antibody Isotype
IgG1
Application
WB, IF, IP

Basic Information

Immunogen
Recombinant protein encompassing a sequence within the center region of human ELP3
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Concentration
2.35 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
Center

Target

Full Name
Elongator Acetyltransferase Complex Subunit 3
Introduction
ELP3 is the catalytic subunit of the histone acetyltransferase elongator complex, which contributes to transcript elongation and also regulates the maturation of projection neurons (Creppe et al., 2009).
Entrez Gene ID
55140
UniProt ID
Q9H9T3
Alternative Names
Elongator Acetyltransferase Complex Subunit 3; EC 2.3.1.48; Elongation Protein 3 Homolog (S. Cerevisiae); Elongation Protein 3 Homolog; Elongator Complex Protein 3; HELP3; KAT9
Research Area
Catalytic tRNA acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation (PubMed:11714725, PubMed:11818576, PubMed:15902492, PubMed:16713582).

The elongator complex is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (PubMed:29415125).

In the elongator complex, acts as a tRNA uridine(34) acetyltransferase by mediating formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (By similarity).

May also act as a protein lysine acetyltransferase by mediating acetylation of target proteins; such activity is however unclear in vivo and recent evidences suggest that ELP3 primarily acts as a tRNA acetyltransferase (PubMed:29415125).

Involved in neurogenesis: regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (PubMed:19185337).

Required for acetylation of GJA1 in the developing cerebral cortex (By similarity).
Biological Process
Central nervous system development Source: UniProtKB
Neuron migration Source: UniProtKB
Positive regulation of cell migration Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: UniProtKB
tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation Source: GO_Central
tRNA wobble uridine modification Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm
Isoform 1: Nucleus
Isoform 2: Nucleus; Cytoplasm
Involvement in disease
Amyotrophic lateral sclerosis (ALS):
A neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are absent. The pathologic hallmarks of the disease include pallor of the corticospinal tract due to loss of motor neurons, presence of ubiquitin-positive inclusions within surviving motor neurons, and deposition of pathologic aggregates. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.

Barznegar, M., Rahimi, K., Mahdavi, P., Menbari, M. N., Darvishi, N., Vahabzadeh, Z., ... & Abdi, M. (2022). Relation between the circular and linear form of the Elongator Acetyltransferase Complex Subunit 3 in the progression of triple‐negative breast cancer. Cell Biochemistry and Function.

Cai, Q., Wang, J., Xie, J., Jiang, D., & Keyhani, N. O. (2022). The Elongator Subunit Elp3 Regulates Development, Stress Tolerance, Cell Cycle, and Virulence in the Entomopathogenic Fungus Beauveria bassiana. Journal of Fungi, 8(8), 834.

Boutoual, R., Jo, H., Heckenbach, I., Tiwari, R., Kasler, H., Lerner, C. A., ... & Verdin, E. (2022). A novel splice variant of Elp3/Kat9 regulates mitochondrial tRNA modification and function. Scientific reports, 12(1), 1-14.

Rojas-Benítez, D., & L. Allende, M. (2020). Elongator subunit 3 (Elp3) is required for zebrafish trunk development. International journal of molecular sciences, 21(3), 925.

Lin, T. Y., Abbassi, N. E. H., Zakrzewski, K., Chramiec-Głąbik, A., Jemioła-Rzemińska, M., Różycki, J., & Glatt, S. (2019). The Elongator subunit Elp3 is a non-canonical tRNA acetyltransferase. Nature communications, 10(1), 1-12.

Abdalla, A., Charli, A., Ghaisas, S., Kondru, N., Malovic, E., Jin, H., ... & Kanthasamy, A. (2019). Mitochondrial Stress Modulates Elongator Protein 3 (ELP3) Function to Enhance Dopaminergic Neurotoxicity in Cell Culture and Animal Models of Parkinson's Disease. The FASEB Journal, 33(S1), 461-1.

Bento-Abreu, A., Jager, G., Swinnen, B., Rué, L., Hendrickx, S., Jones, A., ... & Robberecht, W. (2018). Elongator subunit 3 (ELP3) modifies ALS through tRNA modification. Human molecular genetics, 27(7), 1276-1289.

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For research use only. Not intended for any clinical use.

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