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Mouse Anti-EML4 Recombinant Antibody (3C10) (CBMAB-A2587-LY)

The product is antibody recognizes EML4. The antibody 3C10 immunoassay techniques such as: WB, ELISA.
See all EML4 antibodies
Published Data
Fig.1 The expression of E-cadherin, vimentin, Snail1, FOXA1, and FOXA2 was analyzed by Western blot analysis in human PDA cell lines.
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Fig.2 Transient inhibition of FOXA1/2 leads to loss of epithelial markers.
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Fig.3 Persistent inhibition of FOXA1/2 induces EMT in PDA cells.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
3C10
Antibody Isotype
IgG1, κ
Application
WB, ELISA

Basic Information

Immunogen
EML4 (AAH08685, 1 a.a. ~ 62 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Echinoderm Microtubule Associated Protein Like 4
Entrez Gene ID
27436
UniProt ID
Q9HC35
Alternative Names
C2orf2; DKFZp686P18118; ELP120; FLJ10942; FLJ32318; ROPP120
Research Area
Essential for the formation and stability of microtubules (MTs) (PubMed:16890222, PubMed:31409757).

Required for the organization of the mitotic spindle and for the proper attachment of kinetochores to MTs (PubMed:25789526).

Promotes the recruitment of NUDC to the mitotic spindle for mitotic progression (PubMed:25789526).
Biological Process
Attachment of spindle microtubules to kinetochore Source: UniProtKB
Cell division Source: UniProtKB-KW
Microtubule-based process Source: UniProtKB
Microtubule cytoskeleton organization Source: GO_Central
Mitotic cell cycle Source: UniProtKB
Mitotic metaphase plate congression Source: UniProtKB
Cellular Location
Cytoskeleton; Spindle; Microtubule organizing center; Cytoplasm; Midbody. Localizes to microtubules (MTs) during interphase with a significantly reduced affinity for MTs during mitosis.
PTM
Phosphorylated during mitosis (PubMed:16890222). Phosphorylation at Ser-144 and Ser-146 promotes its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757).
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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