Sign in or Register   Sign in or Register
  |  

Rat Anti-EOMES Recombinant Antibody (Dan11mag) (CBMAB-E0275-FY)

This product is rat antibody that recognizes EOMES. The antibody Dan11mag can be used for immunoassay techniques such as: FC, IHC-P, WB.
See all EOMES antibodies
Published Data

Summary

Host Animal
Rat
Specificity
Mouse
Clone
Dan11mag
Antibody Isotype
IgG2a, κ
Application
FC, IHC-P, WB

Basic Information

Specificity
Mouse
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Preservative
0.09% Sodium azide
Concentration
0.5 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Eomesodermin
Introduction
This gene belongs to the TBR1 (T-box brain protein 1) sub-family of T-box genes that share the common DNA-binding T-box domain. The encoded protein is a transcription factor which is crucial for embryonic development of mesoderm and the central nervous system in vertebrates. The protein may also be necessary for the differentiation of effector CD8+ T cells which are involved in defense against viral infections. A similar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation. Alternative splicing results in multiple transcript variants.
Entrez Gene ID
UniProt ID
Alternative Names
Tbr2; TBR-2; C77258
Research Area
Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.
Biological Process
Adaptive immune response Source: UniProtKB-KW
Brain development Source: UniProtKB
CD8-positive, alpha-beta T cell differentiation involved in immune response Source: UniProtKB
Cell differentiation involved in embryonic placenta development Source: UniProtKB
Cell fate specification Source: GO_Central
Endodermal cell fate specification Source: GO_Central
Endoderm formation Source: UniProtKB
Heart looping Source: GO_Central
Mesodermal to mesenchymal transition involved in gastrulation Source: UniProtKB
Mesoderm formation Source: UniProtKB
Negative regulation of DNA-binding transcription factor activity Source: BHF-UCL
Negative regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: MGI
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus
Involvement in disease
A translocation t(3;10)(p24;q23) located 215 kb 3' to the EOMES gene but leading to loss of its expression was identified in a large consanguineous family. Homozygous silencing produces microcephaly associated with corpus callosum agenesis, bilateral polymicrogyria, ventricular dilatation and a small cerebellum.

Wildner, N. H., Walker, A., Brauneck, F., Ditt, V., Peine, S., Huber, S., ... & Zur Wiesch, J. S. (2022). Transcriptional pattern analysis of virus-specific CD8+ T cells in hepatitis C infection: Increased expression of TOX and Eomesodermin during and after persistent antigen recognition. Frontiers in Immunology, 13.

Harland, L. T., Simon, C. S., Senft, A. D., Costello, I., Greder, L., Imaz-Rosshandler, I., ... & Robertson, E. J. (2021). The T-box transcription factor Eomesodermin governs haemogenic competence of yolk sac mesodermal progenitors. Nature cell biology, 23(1), 61-74.

Chen, S., Zhang, J., Yu, W. B., Zhuang, J. C., Xiao, W., Wu, Z. Y., & Xiao, B. G. (2021). Eomesodermin in CD4+ T cells is essential for Ginkgolide K ameliorating disease progression in experimental autoimmune encephalomyelitis. International journal of biological sciences, 17(1), 50.

Ohayon, D. E., Donmez, O., Parameswaran, S., Kottyan, L. C., Weirauch, M. T., & Waggoner, S. N. (2020). Type 2 cytokines trigger eomesodermin-associated pathway conferring robust interferon-g expression in human NK cells.

Zhang, Z., Li, F., Tian, Y., Cao, L., Gao, Q., Zhang, C., ... & Zhang, Y. (2020). Metformin enhances the antitumor activity of CD8+ T lymphocytes via the AMPK–miR-107–Eomes–PD-1 pathway. The Journal of Immunology, 204(9), 2575-2588.

Harland, L., Simon, C., Senft, A., Costello, I., Greder, L., Imaz-Rosshandler, I., ... & Robertson, E. (2020). 2018–EOMESODERMIN GOVERNS THE HEMOGENIC COMPETENCE OF MURINE YOLK-SAC MESODERMAL PROGENITORS. Experimental Hematology, 88, S34.

Harada, M., Nghia, V. T., Nakao, A., Tanigaki, R., Fukuoka, N., Nishida, A., & Kataoka, T. (2020). Eomesodermin promotes interaction of RelA and NFATc2 with the Ifng promoter and multiple conserved noncoding sequences across the Ifng locus in mouse lymphoma BW5147 cells. Immunology Letters, 225, 33-43.

Gruarin, P., Maglie, S., De Simone, M., Häringer, B., Vasco, C., Ranzani, V., ... & Geginat, J. (2019). Eomesodermin controls a unique differentiation program in human IL‐10 and IFN‐γ coproducing regulatory T cells. European journal of immunology, 49(1), 96-111.

Reiser, J., Sadashivaiah, K., Furusawa, A., Banerjee, A., & Singh, N. (2019). Eomesodermin driven IL-10 production in effector CD8+ T cells promotes a memory phenotype. Cellular immunology, 335, 93-102.

Ask a question We look forward to hearing from you.
0 reviews or Q&As
Loading...
Have you used Rat Anti-EOMES Recombinant Antibody (Dan11mag)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare