EOMES Antibodies

Structure of EOMES

The molecular weight of the T-box transcription factor protein encoded by the EOMES gene is approximately 65-70 kDa, and its precise value fluctuates slightly due to differences in amino acid sequences among species and the presence of different transcripts.

This protein is composed of multiple functional domains, with its core being a highly conserved T-box domain (containing approximately 180 amino acids), which is responsible for specifically recognizing and binding to the target DNA sequence. This domain folds to form a unique three-dimensional conformation, with its secondary structure mainly composed of α -helices and β -folds, creating a binding interface that precisely interacts with the large grooves of DNA. Apart from the core structural domain, the regulatory region sequences at the amino and carboxyl ends vary among different species. These variations affect its transcriptional activity and the specificity of its interactions with other cofactors.

Fig. 1 The model depicting the role of Eomes during iNKT development in the thymus.¹

Key structural properties of EOMES:

• Contains a highly conserved T-box domain
• This domain is composed of an α-helix and a β -fold to form the DNA binding interface
• Bind to the target sequence through specific DNA recognition helices
• Protein N and C regulatory regions influence the transcriptional activity and specificity

Functions of EOMES

The main function of the EOMES gene is to regulate cell fate determination as a transcription factor. However, it is also widely involved in various physiological and pathological processes, including immune responses and tumorigenesis.

Compared with other synergistic transcription factors (such as T-bet), the regulation of target genes by EOMES usually shows more fundamental and earlier initiation characteristics, which is consistent with its "pioneer" role in cell fate determination.

Applications of EOMES and EOMES Antibody in Literature

1. Thelen, Benedikt, et al. "Eomes is sufficient to regulate IL-10 expression and cytotoxic effector molecules in murine CD4+ T cells." Frontiers in Immunology 14 (2023): 1058267. https://doi.org/10.3389/fimmu.2023.1058267

The article indicates that through a new genetic method, it was discovered that Eomes in CD4+ T cells can initiate a unique transcriptional program that partially overlaps with T-BET, which can not only induce the anti-inflammatory factor IL-10 but also work in synergy with T-BET to endow cytotoxic functions.

2. Wong, Pamela, et al. "T-BET and EOMES sustain mature human NK cell identity and antitumor function." The Journal of clinical investigation 133.13 (2023). https://doi.org/10.1172/JCI162530

Research reveals that EOMES and T-BET maintain the function of human NK cells through specific transcriptional programs. After deletion, the anti-tumor function, proliferation and cytokine response of NK cells are impaired, and there is a tendency to transform to other lineages such as ILC-3.

3. Llaó-Cid, Laura, et al. "EOMES is essential for antitumor activity of CD8+ T cells in chronic lymphocytic leukemia." Leukemia 35.11 (2021): 3152-3162. https://doi.org/10.1038/s41375-021-01198-1

Research has found that the EOMES gene variations associated with leukemia risk actually function by influencing CD8+ T cells. EOMES are crucial for the expansion of such cells, and their absence will directly weaken the body's immune ability to control leukemia.

4. Verma, Riva, et al. "Eomes expression defines group 1 innate lymphoid cells during metastasis in human and mouse." Frontiers in immunology 11 (2020): 1190. https://doi.org/10.3389/fimmu.2020.01190

Research has found that during the process of cancer metastasis, NK cells will be transformed into ILC1 along with the down-regulation of Eomes expression. This ILC1 with low expression of Eomes has weakened cytotoxicity, leading to a decline in herd immune surveillance function and thereby promoting disease progression.

5. Shimizu, Kanako, et al. "Eomes transcription factor is required for the development and differentiation of invariant NKT cells." Communications Biology 2.1 (2019): 150. https://doi.org/10.1038/s42003-019-0389-3

Research has found that the transcription factor Eomes not only regulates the differentiation of iNKT cells in the thymus into the NKT1 subtype, but also dominates their differentiation into KLRG1+ effector memory-like cells after peripheral activation, revealing its dual new role in iNKT cells.

Creative Biolabs: EOMES Antibodies for Research

Creative Biolabs specializes in the production of high-quality EOMES antibodies for research and industrial applications. Our portfolio includes monoclonal antibodies tailored for ELISA, Flow Cytometry, Western blot, immunohistochemistry, and other diagnostic methodologies.

• Custom EOMES Antibody Development: Tailor-made solutions to meet specific research requirements.
• Bulk Production: Large-scale antibody manufacturing for industry partners.
• Technical Support: Expert consultation for protocol optimization and troubleshooting.
• Aliquoting Services: Conveniently sized aliquots for long-term storage and consistent experimental outcomes.

For more details on our EOMES antibodies, custom preparations, or technical support, contact us at email.