Mouse Anti-Gro Monoclonal Antibody (1) (CBMAB-0482-LY)

Name: Mouse Anti-Gro Monoclonal Antibody (1)
SKU: CBMAB-0482-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-Gro Monoclonal Antibody (1) (CBMAB-0482-LY) in IHC

(A). Additional RDGN transcription factors are expressed in larval and/or adult IPCs (Larval transcriptome data is taken from Cao et al., (2014) [39] while adult transcriptome data was generated for this study. (B-W) Gro, Tsh, Toy, Dac and Ey are readily detected in L3 and adult IPCs, only Eyg exhibited no detectable protein expression in larval or adult IPCs. Adult Dilp2-GAL4R> CD8GFP flies were reared to sexual maturity and brains were dissected and stained with available ABs directed against RDGN TFs. ...View More

Winant, M., Buhler, K., Clements, J., De Groef, S., Hens, K., Vulsteke, V., & Callaerts, P. (2022). Genome-wide analysis identifies Homothorax and Extradenticle as regulators of insulin in Drosophila Insulin-Producing cells. PLoS genetics, 18(9), e1010380. https://doi.org/10.1371/journal.pgen.1010380

Mouse Anti-Gro Monoclonal Antibody (1) (CBMAB-0482-LY) in IF

(A-C) Confocal image of a PCNA-GFP third instar wandering larval wing imaginal disc, stained for pGro (red; A,C). Cells in S phase are GFP-positive (green; B,C). (D-I) Confocal images of third instar wild-type wandering larval wing (D-F) and eye (G-I) imaginal discs, stained for pGro (turquoise in D,G,G'; blue in F,I), Gro (green; E,F,H-I) and EdU (red; D-I). (D,E,G-H') Magnified views of the boxed regions in F and I, respectively. Arrows (G-I) point at the stripe of EdU-positive, S-phase cells posterior to the morphogenetic furrow. Insets in (D,E,G,H) show magnified views of individual cells stained either for pGro and EdU (D,G) or for Gro and EdU (E,H). ...View More

Bar-Cohen, S., Martínez Quiles, M. L., Baskin, A., Dawud, R., Jennings, B. H., & Paroush, Z. (2023). Normal cell cycle progression requires negative regulation of E2F1 by Groucho during S phase and its relief at G2 phase. Development (Cambridge, England), 150(11), dev201041. https://doi.org/10.1242/dev.201041

Mouse Anti-Gro Monoclonal Antibody (1) (CBMAB-0482-LY) in IF

Rpn12R-GFP germarium stained for GFP or wildtype germaria stained for blanks or gro shown in the green channel or in white, and for DAPI (blue) and vasa (magenta) as indicated. ...View More

Rust, K., Byrnes, L. E., Yu, K. S., Park, J. S., Sneddon, J. B., Tward, A. D., & Nystul, T. G. (2020). A single-cell atlas and lineage analysis of the adult Drosophila ovary. Nature communications, 11(1), 5628. https://doi.org/10.1038/s41467-020-19361-0

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

Application

IF, WB, IP

Immunogen

Recombinant Gro (aa 120-380) GST fusion protein.

Specificity

Common fruit fly

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

groucho protein

Introduction

Transcriptional corepressor that regulates transcription when recruited to specific target DNA by hairy-related bHLH proteins. Maternally required for neurogenesis; in the segregation of the neuroectoderm. Directly or indirectly interacts with Notch and Delta.

Entrez Gene ID

43162

UniProt ID

P16371

Alternative Names

CG8384; Groucho; m9/m10; E(spl)gro; E(spl)m9/10

Function

Biological Process

Cell differentiation Source: UniProtKB-KW
Epidermal growth factor receptor signaling pathway Source: FlyBase
Fibroblast growth factor receptor signaling pathway Source: FlyBase
Negative regulation of canonical Wnt signaling pathway Source: UniProtKB
Negative regulation of Toll signaling pathway Source: FlyBase
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: FlyBase
Nervous system development Source: FlyBase
Regulation of transcription, DNA-templated Source: FlyBase
Sex determination, primary response to X:A ratio Source: FlyBase
Torso signaling pathway Source: FlyBase
Wnt signaling pathway Source: UniProtKB-KW

Cellular Location

Nucleus

PTM

Ubiquitinated by XIAP/BIRC4.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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