Mouse Anti-CD2AP Recombinant Antibody (BR083) (CBMAB-BR083LY)

Name: Mouse Anti-CD2AP Recombinant Antibody (BR083)
SKU: CBMAB-BR083LY
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Published Data

Mouse Anti-CD2AP Recombinant Antibody (BR083) (CBMAB-BR083LY) in WB

Effect of overexpression or inhibition of bta-miR-199a-3p on CD2AP protein expression: is the result of Western blot. ...View More

Li, Y., Ren, Q., Wang, X., Luoreng, Z., & Wei, D. (2022). Bta-miR-199a-3p inhibits LPS-induced inflammation in bovine mammary epithelial cells via the PI3K/AKT/NF-κB signaling pathway. Cells, 11(21), 3518. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CD2AP Recombinant Antibody (BR083) (CBMAB-BR083LY) in IHC

Immunohistochemical expression of CD2AP in IGC, DGC, paired normal tissue, normal gastric lamina propria, and normal gastric epithelium. ...View More

Xie, W., Chen, C., Han, Z., Huang, J., Liu, X., Chen, H., ... & Xue, X. (2020). CD2AP inhibits metastasis in gastric cancer by promoting cellular adhesion and cytoskeleton assembly. Molecular carcinogenesis, 59(4), 339-352. Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CD2AP Recombinant Antibody (BR083) (CBMAB-BR083LY) in WB

Protein blots from four independent lysates were probed for CD2AP, WT1, VDR, YY1, Sox2, and Glyceraldehyde -3-phosphate dehydrogenase (GAPDH). ...View More

Jha, A., Saha, S., Ayasolla, K., Vashistha, H., Malhotra, A., Skorecki, K., & Singhal, P. C. (2020). MiR193a modulation and podocyte phenotype. Cells, 9(4), 1004. Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

BR083

Application

WB, IP, ELISA, IF, IHC-P

Immunogen

Amino acids 350-639 of CD2AP of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

CD2 associated protein

Introduction

CD2AP (CD2 Associated Protein) is a Protein Coding gene. Diseases associated with CD2AP include Focal Segmental Glomerulosclerosis 3 and Sporadic Idiopathic Steroid-Resistant Nephrotic Syndrome With Focal Segmental Hyalinosis. Among its related pathways are Primary Focal Segmental Glomerulosclerosis FSGS and Nephrin interactions. Gene Ontology (GO) annotations related to this gene include SH3 domain binding and structural constituent of cytoskeleton. An important paralog of this gene is SH3KBP1.

Entrez Gene ID

Human	23607
Mouse	12488
Rat	316258

UniProt ID

Human	Q9Y5K6
Mouse	Q9JLQ0
Rat	F1LRS8

Function

Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton (PubMed:10339567).
In collaboration with CBLC, modulates the rate of RET turnover and may act as regulatory checkpoint that limits the potency of GDNF on neuronal survival. Controls CBLC function, converting it from an inhibitor to a promoter of RET degradation (By similarity).
May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell (By similarity).
May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis (PubMed:15800069).
Plays a role in epithelial cell junctions formation (PubMed:22891260).

Biological Process

Actin filament organization Source: UniProtKB
Cell-cell adhesion Source: Ensembl
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB-KW
Negative regulation of small GTPase mediated signal transduction Source: UniProtKB
Negative regulation of transforming growth factor beta1 production Source: Ensembl
Positive regulation of protein localization to nucleus Source: Ensembl
Positive regulation of protein secretion Source: UniProtKB
Proteasome-mediated ubiquitin-dependent protein catabolic process Source: Ensembl
Protein-containing complex assembly Source: ProtInc
Regulation of actin cytoskeleton reorganization Source: Ensembl
Regulation of receptor-mediated endocytosis Source: Ensembl
Signal transduction Source: ProtInc
Substrate-dependent cell migration, cell extension Source: ProtInc
Vesicle organization Source: Ensembl

Cellular Location

Cytoskeleton; Ruffle; Cell junction. Colocalizes with F-actin and BCAR1/p130Cas in membrane ruffles (PubMed:10339567). Located at podocyte slit diaphragm between podocyte foot processes (By similarity). During late anaphase and telophase, concentrates in the vicinity of the midzone microtubules and in the midbody in late telophase (PubMed:15800069).

Involvement in disease

Focal segmental glomerulosclerosis 3 (FSGS3): A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and progressive decline in renal function. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.

PTM

Phosphorylated on tyrosine residues; probably by c-Abl, Fyn and c-Src.

Furusawa, K., Takasugi, T., Chiu, Y. W., Hori, Y., Tomita, T., Fukuda, M., & Hisanaga, S. I. (2019). CD2-associated protein (CD2AP) overexpression accelerates amyloid precursor protein (APP) transfer from early endosomes to the lysosomal degradation pathway. Journal of Biological Chemistry, 294(28), 10886-10899.

Tao, Q. Q., Chen, Y. C., & Wu, Z. Y. (2019). The role of CD2AP in the Pathogenesis of Alzheimer's Disease. Aging and disease, 10(4), 901.

Ha, T. S., & Ha, D. S. (2019). Ginseng total saponin attenuates podocyte apoptosis induced by diabetic conditions through the recovery of CD2-associated protein. Journal of medicinal food, 22(2), 170-177.

Tossidou, I., Teng, B., Worthmann, K., Müller-Deile, J., Jobst-Schwan, T., Kardinal, C., ... & Schiffer, M. (2019). Tyrosine phosphorylation of CD2AP affects stability of the slit diaphragm complex. Journal of the American Society of Nephrology, 30(7), 1220-1237.

Ojelade, S. A., Lee, T. V., Giagtzoglou, N., Yu, L., Ugur, B., Li, Y., ... & Shulman, J. M. (2019). cindr, the Drosophila homolog of the CD2AP Alzheimer’s disease risk gene, is required for synaptic transmission and proteostasis. Cell reports, 28(7), 1799-1813.

Takano, T., Bareke, E., Takeda, N., Aoudjit, L., Baldwin, C., Pisano, P., ... & Gupta, I. R. (2019). Recessive mutation in CD2AP causes focal segmental glomerulosclerosis in humans and mice. Kidney international, 95(1), 57-61.

Zhang, H., Zhang, C., Tang, H., Gao, S., Sun, F., Yang, Y., ... & Li, C. (2018). CD2‐Associated Protein Contributes to Hepatitis C, Virus Propagation and Steatosis by Disrupting Insulin Signaling. Hepatology, 68(5), 1710-1725.

Panni, S. (2018). CD2AP (CD2 associated protein). Atlas of Genetics and Cytogenetics in Oncology and Haematology.

Tsuji, K., Păunescu, T. G., Suleiman, H., Xie, D., Mamuya, F. A., Miner, J. H., & Lu, H. A. J. (2017). Re-characterization of the glomerulopathy in CD2AP deficient mice by high-resolution helium ion scanning microscopy. Scientific reports, 7(1), 1-13.

Saurus, P., Tolvanen, T. A., Lindfors, S., Kuusela, S., Holthöfer, H., Lehtonen, E., & Lehtonen, S. (2017). Inhibition of SHIP2 in CD2AP-deficient podocytes ameliorates reactive oxygen species generation but aggravates apoptosis. Scientific reports, 7(1), 1-14.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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