Mouse Anti-ADGRE2 Recombinant Antibody (V2-261270) (CBMAB-C0813-LY)

Name: Mouse Anti-ADGRE2 Recombinant Antibody (V2-261270)
SKU: CBMAB-C0813-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ADGRE2 Recombinant Antibody (2A1) (CBMAB-C0813-LY) in ELISA

CHO-K1 cells were transiently transfected with the hCD4-myc or negative control EMR2-myc expression construct. Production of sCD4 is evaluated by ELISA analysis. Conditioned medium (CM) and cell lysates (CL) were probed with 2A1 (specific to EMR2), anti-CD4 and anti-myc mAbs in WB analysis. Kruskal-Wallis test across all three groups of 1X and 10X concentration shows p = 0.027. Dunn's multiple comparisons post-test p values between groups show ***P<0.001. ...View More

Tseng, W. Y., Huang, Y. S., Chiang, N. Y., Chou, Y. P., Wu, Y. J. J., Luo, S. F., ... & Lin, H. H. (2013). Increased soluble CD4 in serum of rheumatoid arthritis patients is generated by matrix metalloproteinase (MMP)-like proteinases. PLoS One, 8(5), e63963.

Mouse Anti-ADGRE2 Recombinant Antibody (2A1) (CBMAB-C0813-LY) in WB

CHO-K1 cells were transiently transfected with the hCD4-myc or negative control EMR2-myc expression construct. Production of sCD4 is evaluated by WB analysis. Conditioned medium (CM) and cell lysates (CL) were probed with 2A1 (specific to EMR2), anti-CD4 and anti-myc mAbs in WB analysis. Kruskal-Wallis test across all three groups of 1X and 10X concentration shows p = 0.027. Dunn's multiple comparisons post-test p values between groups show ***P<0.001. ...View More

Mouse Anti-ADGRE2 Recombinant Antibody (2A1) (CBMAB-C0813-LY) in WB

The hydrophilic characteristics of membrane-associated EMR2-NTF. (A) WB analysis of supernatant and cell lysate samples subjected to Triton X-114 phase separation. Supernatant and cell lysate were collected from PFO-treated CHO-K1 cells expressing EMR2-WT-myc. Ctl: input control protein samples before Triton X-114 phase separation; AP: aqueous phase; DP1 and 2: the first and second detergent phases, respectively. (B) WB analysis of membrane fraction of EMR2-WT-myc expressing cells after PBS, high salt (1 M NaCl) and high pH (100 mM Na2CO3, pH 11) treatment. S and L represent supernatant and cell pellets, respectively. The arrow indicates the specific Gαi1,2 protein band. (C) WB analysis of membrane fraction of thrombin-treated (T+) or untreated (T−) EMR2-WT-PAR1-myc expressing cells, followed by PBS, high salt (1 M NaCl) and high pH (100 mM Na2CO3, pH 11) treatment. Blots were probed with indicated mAbs. ...View More

Huang, Y. S., Chiang, N. Y., Chang, G. W., & Lin, H. H. (2018). Membrane-association of EMR2/ADGRE2-NTF is regulated by site-specific N-glycosylation. Scientific reports, 8(1), 4532.

Mouse Anti-ADGRE2 Recombinant Antibody (2A1) (CBMAB-C0813-LY) in IF and FC

Membrane-association of EMR2-NTF occurs in post-ER compartments. (A) Schematic representation of the chimeric EMR2-CD4 fusion proteins including EMR2-WT-CD4-HA-AATN (1), EMR2-S518A-CD4-HA-AATN (2), EMR2-WT-CD4 -HA-KKTN (3) and EMR2-S518A-CD4-HA-KKTN (4). The CD4 TM region and HA tag are represented by a single gray zig-zag line and a black hexagon, respectively. KKTN and AATN indicate the C-terminal ER retention signal peptide and control peptide, respectively. (B) WB analysis of total cell lysates of CHO-K1 cells expressing the respective chimeric EMR2-CD4 fusion proteins as indicated by the numbers in (A). Lysates were digested without (left panel) or with (right panel) Endo-H. The blot was probed with the 2A1 mAb. (C) FACS analysis of EMR2-NTF expression levels on cell surface and permeabilized whole cell of CKO-K1 cells expressing the respective chimeric EMR2-CD4 fusion proteins as indicated by the numbers in (A). (D) Confocal immunofluorescence analysis of EMR2-NTF expression patterns on cell surface (left panel) and whole cell (right panel) of CKO-K1 cells expressing the respective chimeric EMR2-CD4 fusion proteins as indicated by the numbers in (A). The red color represents EMR2-NTF stained by 2A1 mAb followed by goat anti-mFc IgG-Alexa Flour 647. The green color represents the ER marker expressed by the transfected pCMV-myc-ER-GFP plasmid, while the blue color indicates the nucleus stained by DAPI. Scale: 10 μm. ...View More

Huang, Y. S., Chiang, N. Y., Chang, G. W., & Lin, H. H. (2018). Membrane-association of EMR2/ADGRE2-NTF is regulated by site-specific N-glycosylation. Scientific reports, 8(1), 4532.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-261270

Application

WB, IHC-P, IHC-F

Immunogen

NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC-F	1:50-1:200
FC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

0.09% sodium azide

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Adhesion G Protein-Coupled Receptor E2

Introduction

This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Entrez Gene ID

30817

UniProt ID

Q9UHX3

Alternative Names

Adhesion G Protein-Coupled Receptor E2; Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 2; EGF-Like Module-Containing Mucin-Like Hormone Receptor-Like 2; EMR2; Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like Sequence 2; EGF-Lik

Function

Cell surface receptor that binds to the chondroitin sulfate moiety of glycosaminoglycan chains and promotes cell attachment. Promotes granulocyte chemotaxis, degranulation and adhesion. In macrophages, promotes the release of inflammatory cytokines, including IL8 and TNF. Signals probably through G-proteins. Is a regulator of mast cell degranulation.

Biological Process

Adenylate cyclase-activating G protein-coupled receptor signaling pathway
Cell adhesion
Cell migration
Cell surface receptor signaling pathway
G protein-coupled receptor signaling pathway
Granulocyte chemotaxis
Inflammatory response
Regulation of mast cell degranulation

Cellular Location

Cell membrane; Ruffle membrane. Localized at the leading edge of migrating cells.

Involvement in disease

Vibratory urticaria (VBU): An autosomal dominant disorder characterized by localized hives and systemic manifestations in response to dermal vibration, with coincident degranulation of mast cells and increased histamine levels in serum.

Topology

Extracellular: 24-540 aa
Helical: 541-561 aa
Cytoplasmic: 562-569 aa
Helical: 570-590 aa
Extracellular: 591-605 aa
Helical: 606-626 aa
Cytoplasmic: 627-644 aa
Helical: 645-665 aa
Extracellular: 666-683 aa
Helical: 684-704 aa
Cytoplasmic: 705-735 aa
Helical: 736-756 aa
Extracellular: 757-760 aa
Helical: 761-781 aa
Cytoplasmic: 782-823 aa

PTM

Autoproteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane beta subunit.

Tseng, W. Y., Wang, W. C., Gordon, S., Ng, K. F., & Lin, H. H. (2021). Stimulation of Vibratory Urticaria-Associated Adhesion-GPCR, EMR2/ADGRE2, Triggers the NLRP3 Inflammasome Activation Signal in Human Monocytes. Frontiers in Immunology, 11, 3359.

Bhudia, N., Desai, S., King, N., Ancellin, N., Grillot, D., Barnes, A. A., & Dowell, S. J. (2020). G protein-coupling of adhesion GPCRs ADGRE2/EMR2 and ADGRE5/CD97, and activation of G protein signalling by an anti-EMR2 antibody. Scientific reports, 10(1), 1-13.

Bhudia, N., Desai, S., King, N., Ancellin, N., Grillot, D., Barnes, A. A., & Dowell, S. J. (2020). Author correction: G protein-coupling of adhesion GPCRs ADGRE2/EMR2 and ADGRE5/CD97, and activation of G protein signalling by an anti-EMR2 antibody. Scientific reports, 10(1), 1-2.

Naranjo, A. N., Bandara, G., Bai, Y., Smelkinson, M. G., Tobío, A., Komarow, H. D., ... & Olivera, A. (2020). Critical Signaling Events in the Mechanoactivation of Human Mast Cells through p. C492Y-ADGRE2. Journal of Investigative Dermatology, 140(11), 2210-2220.

Huang, Y. S., Hu, C. H., Tseng, W. Y., Cheng, C. H., Stacey, M., Gordon, S., ... & Lin, H. H. (2017). Activation of Adhesion GPCR EMR2/ADGRE2 Induces Macrophage Differentiation and Inflammatory Responses via Gα16/Akt/MAPK/NF-κB Signaling Pathways. Frontiers in immunology, 8, 373.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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