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Mouse Anti-ADGRE2 Recombinant Antibody (V2-261270) (CBMAB-C0813-LY)

This product is antibody recognizes ADGRE2. The antibody 2A1 immunoassay techniques such as: IHC-F, WB, IHC-P, FC.
See all ADGRE2 antibodies
Published Data
Mouse Anti-ADGRE2 Recombinant Antibody (2A1) (CBMAB-C0813-LY) in ELISA
CHO-K1 cells were transiently transfected with the hCD4-myc or negative control EMR2-myc expression construct. Production of sCD4 is evaluated by ELISA analysis. Conditioned medium (CM) and cell lysates (CL) were probed with 2A1 (specific to EMR2), anti-CD4 and anti-myc mAbs in WB analysis. Kruskal-Wallis test across all three groups of 1X and 10X concentration shows p = 0.027. Dunn's multiple comparisons post-test p values between groups show ***P<0.001. ...View More
Tseng, W. Y., Huang, Y. S., Chiang, N. Y., Chou, Y. P., Wu, Y. J. J., Luo, S. F., ... & Lin, H. H. (2013). Increased soluble CD4 in serum of rheumatoid arthritis patients is generated by matrix metalloproteinase (MMP)-like proteinases. PLoS One, 8(5), e63963.
Mouse Anti-ADGRE2 Recombinant Antibody (2A1) (CBMAB-C0813-LY) in WB
CHO-K1 cells were transiently transfected with the hCD4-myc or negative control EMR2-myc expression construct. Production of sCD4 is evaluated by WB analysis. Conditioned medium (CM) and cell lysates (CL) were probed with 2A1 (specific to EMR2), anti-CD4 and anti-myc mAbs in WB analysis. Kruskal-Wallis test across all three groups of 1X and 10X concentration shows p = 0.027. Dunn's multiple comparisons post-test p values between groups show ***P<0.001. ...View More
Tseng, W. Y., Huang, Y. S., Chiang, N. Y., Chou, Y. P., Wu, Y. J. J., Luo, S. F., ... & Lin, H. H. (2013). Increased soluble CD4 in serum of rheumatoid arthritis patients is generated by matrix metalloproteinase (MMP)-like proteinases. PLoS One, 8(5), e63963.
Mouse Anti-ADGRE2 Recombinant Antibody (2A1) (CBMAB-C0813-LY) in WB
The hydrophilic characteristics of membrane-associated EMR2-NTF. (A) WB analysis of supernatant and cell lysate samples subjected to Triton X-114 phase separation. Supernatant and cell lysate were collected from PFO-treated CHO-K1 cells expressing EMR2-WT-myc. Ctl: input control protein samples before Triton X-114 phase separation; AP: aqueous phase; DP1 and 2: the first and second detergent phases, respectively. (B) WB analysis of membrane fraction of EMR2-WT-myc expressing cells after PBS, high salt (1 M NaCl) and high pH (100 mM Na2CO3, pH 11) treatment. S and L represent supernatant and cell pellets, respectively. The arrow indicates the specific Gαi1,2 protein band. (C) WB analysis of membrane fraction of thrombin-treated (T+) or untreated (T−) EMR2-WT-PAR1-myc expressing cells, followed by PBS, high salt (1 M NaCl) and high pH (100 mM Na2CO3, pH 11) treatment. Blots were probed with indicated mAbs. ...View More
Huang, Y. S., Chiang, N. Y., Chang, G. W., & Lin, H. H. (2018). Membrane-association of EMR2/ADGRE2-NTF is regulated by site-specific N-glycosylation. Scientific reports, 8(1), 4532.
Mouse Anti-ADGRE2 Recombinant Antibody (2A1) (CBMAB-C0813-LY) in IF and FC
Membrane-association of EMR2-NTF occurs in post-ER compartments. (A) Schematic representation of the chimeric EMR2-CD4 fusion proteins including EMR2-WT-CD4-HA-AATN (1), EMR2-S518A-CD4-HA-AATN (2), EMR2-WT-CD4 -HA-KKTN (3) and EMR2-S518A-CD4-HA-KKTN (4). The CD4 TM region and HA tag are represented by a single gray zig-zag line and a black hexagon, respectively. KKTN and AATN indicate the C-terminal ER retention signal peptide and control peptide, respectively. (B) WB analysis of total cell lysates of CHO-K1 cells expressing the respective chimeric EMR2-CD4 fusion proteins as indicated by the numbers in (A). Lysates were digested without (left panel) or with (right panel) Endo-H. The blot was probed with the 2A1 mAb. (C) FACS analysis of EMR2-NTF expression levels on cell surface and permeabilized whole cell of CKO-K1 cells expressing the respective chimeric EMR2-CD4 fusion proteins as indicated by the numbers in (A). (D) Confocal immunofluorescence analysis of EMR2-NTF expression patterns on cell surface (left panel) and whole cell (right panel) of CKO-K1 cells expressing the respective chimeric EMR2-CD4 fusion proteins as indicated by the numbers in (A). The red color represents EMR2-NTF stained by 2A1 mAb followed by goat anti-mFc IgG-Alexa Flour 647. The green color represents the ER marker expressed by the transfected pCMV-myc-ER-GFP plasmid, while the blue color indicates the nucleus stained by DAPI. Scale: 10 μm. ...View More
Huang, Y. S., Chiang, N. Y., Chang, G. W., & Lin, H. H. (2018). Membrane-association of EMR2/ADGRE2-NTF is regulated by site-specific N-glycosylation. Scientific reports, 8(1), 4532.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-261270
Antibody Isotype
IgG1
Application
WB, IHC-P, IHC-F

Basic Information

Immunogen
NIH-3T3 cells stably transfected with EMR2 (EGF1-5) cDNA
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IHC-F1:50-1:200
FC1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS
Preservative
0.09% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Adhesion G Protein-Coupled Receptor E2
Introduction
This gene encodes a member of the class B seven-span transmembrane (TM7) subfamily of G-protein coupled receptors. These proteins are characterized by an extended extracellular region with a variable number of N-terminal epidermal growth factor-like domains coupled to a TM7 domain via a mucin-like spacer domain. The encoded protein is expressed mainly in myeloid cells where it promotes cell-cell adhesion through interaction with chondroitin sulfate chains. This gene is situated in a cluster of related genes on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
Entrez Gene ID
30817
UniProt ID
Q9UHX3
Alternative Names
Adhesion G Protein-Coupled Receptor E2; Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 2; EGF-Like Module-Containing Mucin-Like Hormone Receptor-Like 2; EMR2; Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like Sequence 2; EGF-Lik
Function
Cell surface receptor that binds to the chondroitin sulfate moiety of glycosaminoglycan chains and promotes cell attachment. Promotes granulocyte chemotaxis, degranulation and adhesion. In macrophages, promotes the release of inflammatory cytokines, including IL8 and TNF. Signals probably through G-proteins. Is a regulator of mast cell degranulation.
Biological Process
Adenylate cyclase-activating G protein-coupled receptor signaling pathway
Cell adhesion
Cell migration
Cell surface receptor signaling pathway
G protein-coupled receptor signaling pathway
Granulocyte chemotaxis
Inflammatory response
Regulation of mast cell degranulation
Cellular Location
Cell membrane; Ruffle membrane. Localized at the leading edge of migrating cells.
Involvement in disease
Vibratory urticaria (VBU): An autosomal dominant disorder characterized by localized hives and systemic manifestations in response to dermal vibration, with coincident degranulation of mast cells and increased histamine levels in serum.
Topology
Extracellular: 24-540 aa
Helical: 541-561 aa
Cytoplasmic: 562-569 aa
Helical: 570-590 aa
Extracellular: 591-605 aa
Helical: 606-626 aa
Cytoplasmic: 627-644 aa
Helical: 645-665 aa
Extracellular: 666-683 aa
Helical: 684-704 aa
Cytoplasmic: 705-735 aa
Helical: 736-756 aa
Extracellular: 757-760 aa
Helical: 761-781 aa
Cytoplasmic: 782-823 aa
PTM
Autoproteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane beta subunit.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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