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Mouse Anti-BSN Recombinant Antibody (SAP7F407) (CBMAB-1228-CN)

This product is a mouse antibody that recognizes BSN of rat. The antibody SAP7F407 can be used for immunoassay techniques such as: IF, IHC-P, IP, WB.
See all BSN antibodies
Published Data
Fig.1 Maximal projection of confocal sections from organs of Corti co-labeled for the presynaptic marker and anchor of the ribbon, bassoon.
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Fig.2 Immunohisotchemistry from WT (A) and KO (B) for Bassoon (green).
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Summary

Host Animal
Mouse
Specificity
Rat, Mouse
Clone
SAP7F407
Antibody Isotype
IgG2a
Application
IF, IHC-P, IP, WB

Basic Information

Immunogen
Recombinant rat Bassoon protein
Specificity
Rat, Mouse
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.09% Sodium azide
Concentration
1 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Bassoon Presynaptic Cytomatrix Protein
Introduction
This protein is a component of the presynaptic cytoskeleton and may be be involved in cytomatrix organization at the site of neurotransmitter release.
Entrez Gene ID
Mouse12217
Rat29138
UniProt ID
MouseO88737
RatO88778
Alternative Names
Znf231
Function
Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:12812759).
After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (PubMed:19380881).
At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity).
Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy by associating with ATG5 (By similarity).
Mediates also synapse to nucleus communication leading to reconfiguration of gene expression by associating with the transcriptional corepressor CTBP1 and by subsequently reducing the size of its pool available for nuclear import (By similarity).
Biological Process
Chemical synaptic transmission Source: ProtInc
Presynapse to nucleus signaling pathway Source: Ensembl
Presynaptic active zone assembly Source: GO_Central
Protein localization to synapse Source: GO_Central
Regulation of synaptic vesicle cycle Source: Ensembl
Cellular Location
Cytoskeleton; Cytoplasm; Presynaptic active zone; Synaptic vesicle membrane. In retina, is localized in the outer plexiform layer at ribbon synapses formed by rods and cones but was absent from basal synaptic contacts formed by cones. In the retinal inner plexiform layer localized to conventional inhibitory GABAergic synapses, made by amacrine cells, but absent from the bipolar cell ribbon synapses.
PTM
Myristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization (By similarity).

Montenegro-Venegas, C., Fienko, S., Anni, D., Pina-Fernández, E., Frischknecht, R., & Fejtova, A. (2021). Bassoon inhibits proteasome activity via interaction with PSMB4. Cellular and Molecular Life Sciences, 78(4), 1545-1563.

Ryl, M., Urbasik, A., Gierke, K., Babai, N., Joachimsthaler, A., Feigenspan, A., ... & Brandstätter, J. H. (2021). Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes. The FASEB Journal, 35(5), e21520.

Ghelani, T., Montenegro-Venegas, C., Fejtova, A., & Dresbach, T. (2021). Nanoscopical analysis reveals an orderly arrangement of the presynaptic scaffold protein Bassoon at the Golgi-apparatus. bioRxiv.

Montenegro-Venegas, C., Guhathakurta, D., Pina-Fernandez, E., Andres-Alonso, M., Plattner, F., Lazarevic, V., ... & Fejtova, A. (2021). Bassoon controls synaptic vesicle pools via regulation of presynaptic phosphorylation and cAMP homeostasis. bioRxiv.

Annamneedi, A., Del Angel, M., Gundelfinger, E. D., Stork, O., & Çalışkan, G. (2021). The Presynaptic Scaffold Protein Bassoon in Forebrain Excitatory Neurons Mediates Hippocampal Circuit Maturation: Potential Involvement of TrkB Signalling. International journal of molecular sciences, 22(15), 7944.

Hoffmann-Conaway, S., Brockmann, M. M., Schneider, K., Annamneedi, A., Rahman, K. A., Bruns, C., ... & Montenegro-Venegas, C. (2020). Parkin contributes to synaptic vesicle autophagy in Bassoon-deficient mice. Elife, 9, e56590.

Babai, N., Gierke, K., Müller, T., Regus‐Leidig, H., Brandstätter, J. H., & Feigenspan, A. (2019). Signal transmission at invaginating cone photoreceptor synaptic contacts following deletion of the presynaptic cytomatrix protein Bassoon in mouse retina. Acta Physiologica, 226(2), e13241.

Annamneedi, A., Caliskan, G., Müller, S., Montag, D., Budinger, E., Angenstein, F., ... & Stork, O. (2018). Ablation of the presynaptic organizer Bassoon in excitatory neurons retards dentate gyrus maturation and enhances learning performance. Brain Structure and Function, 223(7), 3423-3445.

Okerlund, N. D., Schneider, K., Leal-Ortiz, S., Montenegro-Venegas, C., Kim, S. A., Garner, L. C., ... & Garner, C. C. (2017). Bassoon controls presynaptic autophagy through Atg5. Neuron, 93(4), 897-913.

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For research use only. Not intended for any clinical use.

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