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Mouse Anti-BSN Recombinant Antibody (CBYY-0846) (CBMAB-0849-YY)

This product is rabbit antibody that recognizes BSN. The antibody CBYY-0846 can be used for immunoassay techniques such as: WB, IF
See all BSN antibodies
Published Data
CBMAB-0849-YY-1
CBMAB-0849-YY in IHC
Outer plexiform layer (OPL) of 2-3 months and>2 years old retinae labeled for AZs (Bsn). ...View More
Gierke, K., Lux, U. T., Regus-Leidig, H., & Brandstätter, J. H. (2023). The first synapse in vision in the aging mouse retina. Frontiers in Cellular Neuroscience, 17.
CBMAB-0849-YY-2
CBMAB-0849-YY in IHC
Protein-retention Expansion Microscopy (proExM) with confocal fluorescence imaging shows that Kv3.3 subunits are present in calyx of Held presynaptic terminal membrane. ...View More
Richardson, A., Ciampani, V., Stancu, M., Bondarenko, K., Newton, S., Steinert, J. R., ... & Forsythe, I. D. (2022). Kv3. 3 subunits control presynaptic action potential waveform and neurotransmitter release at a central excitatory synapse. Elife, 11, e75219.

Summary

Host Animal
Mouse
Specificity
Rat, Mouse
Clone
CBYY-0846
Antibody Isotype
IgG2b, κ
Application
IF, P, IP, WB

Basic Information

Immunogen
Recombinant protein corresponding to residues near the central region of rat Bassoon.
Host Species
Mouse
Specificity
Rat, Mouse
Antibody Isotype
IgG2b, κ
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
IF(ICC)1:100-1:500
WB1:1,000
IHC-P1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Lyophilized
Buffer
PBS
Preservative
None
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
N-Terminus

Target

Full Name
Bassoon Presynaptic Cytomatrix Protein
Introduction
Neurotransmitters are released from a specific site in the axon terminal called the active zone, which is composed of synaptic vesicles and a meshwork of cytoskeleton underlying the plasma membrane. The protein encoded by this gene is thought to be a scaffolding protein involved in organizing the presynaptic cytoskeleton. The gene is expressed primarily in neurons in the brain. A similar gene product in rodents is concentrated in the active zone of axon terminals and tightly associated with cytoskeletal structures, and is essential for regulating neurotransmitter release from a subset of synapses.
Entrez Gene ID
Human8927
Mouse12217
Rabbit100352843
UniProt ID
HumanQ9UPA5
MouseO88737
Alternative Names
Bassoon Presynaptic Cytomatrix Protein; Zinc Finger Protein 231; Neuronal Double Zinc Finger Protein; ZNF231; Bassoon (Presynaptic Cytomatrix Protein); Protein Bassoon; KIAA0434;
Function
Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:12812759).
After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (PubMed:19380881).
At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity).
Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy by associating with ATG5 (By similarity).
Mediates also synapse to nucleus communication leading to reconfiguration of gene expression by associating with the transcriptional corepressor CTBP1 and by subsequently reducing the size of its pool available for nuclear import (By similarity).
Biological Process
Chemical synaptic transmission Source: ProtInc
Presynapse to nucleus signaling pathway Source: Ensembl
Presynaptic active zone assembly Source: GO_Central
Protein localization to synapse Source: GO_Central
Regulation of synaptic vesicle cycle Source: Ensembl
Cellular Location
Cytoskeleton; Cytoplasm; Presynaptic active zone; Synaptic vesicle membrane. In retina, is localized in the outer plexiform layer at ribbon synapses formed by rods and cones but was absent from basal synaptic contacts formed by cones. In the retinal inner plexiform layer localized to conventional inhibitory GABAergic synapses, made by amacrine cells, but absent from the bipolar cell ribbon synapses.
PTM
Myristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization (By similarity).

Montenegro-Venegas, C., Fienko, S., Anni, D., Pina-Fernández, E., Frischknecht, R., & Fejtova, A. (2021). Bassoon inhibits proteasome activity via interaction with PSMB4. Cellular and Molecular Life Sciences, 78(4), 1545-1563.

Ryl, M., Urbasik, A., Gierke, K., Babai, N., Joachimsthaler, A., Feigenspan, A., ... & Brandstätter, J. H. (2021). Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes. The FASEB Journal, 35(5), e21520.

Ghelani, T., Montenegro-Venegas, C., Fejtova, A., & Dresbach, T. (2021). Nanoscopical analysis reveals an orderly arrangement of the presynaptic scaffold protein Bassoon at the Golgi-apparatus. bioRxiv.

Montenegro-Venegas, C., Guhathakurta, D., Pina-Fernandez, E., Andres-Alonso, M., Plattner, F., Lazarevic, V., ... & Fejtova, A. (2021). Bassoon controls synaptic vesicle pools via regulation of presynaptic phosphorylation and cAMP homeostasis. bioRxiv.

Annamneedi, A., Del Angel, M., Gundelfinger, E. D., Stork, O., & Çalışkan, G. (2021). The Presynaptic Scaffold Protein Bassoon in Forebrain Excitatory Neurons Mediates Hippocampal Circuit Maturation: Potential Involvement of TrkB Signalling. International journal of molecular sciences, 22(15), 7944.

Hoffmann-Conaway, S., Brockmann, M. M., Schneider, K., Annamneedi, A., Rahman, K. A., Bruns, C., ... & Montenegro-Venegas, C. (2020). Parkin contributes to synaptic vesicle autophagy in Bassoon-deficient mice. Elife, 9, e56590.

Babai, N., Gierke, K., Müller, T., Regus‐Leidig, H., Brandstätter, J. H., & Feigenspan, A. (2019). Signal transmission at invaginating cone photoreceptor synaptic contacts following deletion of the presynaptic cytomatrix protein Bassoon in mouse retina. Acta Physiologica, 226(2), e13241.

Annamneedi, A., Caliskan, G., Müller, S., Montag, D., Budinger, E., Angenstein, F., ... & Stork, O. (2018). Ablation of the presynaptic organizer Bassoon in excitatory neurons retards dentate gyrus maturation and enhances learning performance. Brain Structure and Function, 223(7), 3423-3445.

Okerlund, N. D., Schneider, K., Leal-Ortiz, S., Montenegro-Venegas, C., Kim, S. A., Garner, L. C., ... & Garner, C. C. (2017). Bassoon controls presynaptic autophagy through Atg5. Neuron, 93(4), 897-913.

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For research use only. Not intended for any clinical use.

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