Mouse Anti-Bsn Recombinant Antibody (CBYC-P031) (CBMAB-P0118-YC)

Name: Mouse Anti-Bsn Recombinant Antibody (CBYC-P031)
SKU: CBMAB-P0118-YC
Rating: 5 (1 reviews)

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Published Data

CBMAB-P0118-YC in IF

Representative images of Cdkl5-WT/KO neurons at DIV14 stained for Bassoon (red,) and MAP2 (blue). Scale bar, 5 μm. ...View More

De Rosa, R., Valastro, S., Cambria, C., Barbiero, I., Puricelli, C., Tramarin, M., ... & Kilstrup-Nielsen, C. (2022). Loss of CDKL5 causes synaptic GABAergic defects that can be restored with the neuroactive steroid pregnenolone-methyl-ether. International Journal of Molecular Sciences, 24(1), 68.

CBMAB-P0118-YC in IF

Representative IF images shown below for Bassoon, Homer-1, and Synaptophysin (N = 2-3, ~700-2500 cells per N) following 5 d of A-769662 (100 nM) and Compound C (30 nM) treatment. DAPI nucleus stain shown in blue, phalloidin cytoskeleton actin stain shown in red, and each protein of interest shown in green. ...View More

Yang, A. J., Mohammad, A., Tsiani, E., Necakov, A., & MacPherson, R. E. (2022). Chronic AMPK activation reduces the expression and alters distribution of synaptic proteins in neuronal SH-SY5Y cells. Cells, 11(15), 2354.

Datasheet

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Basic Information

Host Animal

Mouse

Clone

CBYC-P031

Application

IP, IF, WB

Immunogen

Recombinant bassoon of rat origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat, Cattle, Dog

Antibody Isotype

IgG2a, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Bassoon Presynaptic Cytomatrix Protein

Introduction

Neurotransmitters are released from a specific site in the axon terminal called the active zone, which is composed of synaptic vesicles and a meshwork of cytoskeleton underlying the plasma membrane. The protein encoded by this gene is thought to be a scaffolding protein involved in organizing the presynaptic cytoskeleton. The gene is expressed primarily in neurons in the brain. A similar gene product in rodents is concentrated in the active zone of axon terminals and tightly associated with cytoskeletal structures, and is essential for regulating neurotransmitter release from a subset of synapses.

Entrez Gene ID

Mouse	12217
Rat	29138

UniProt ID

Mouse	O88737
Rat	O88778

Alternative Names

Bassoon Presynaptic Cytomatrix Protein; Zinc Finger Protein 231; Neuronal Double Zinc Finger Protein; ZNF231; Bassoon (Presynaptic Cytomatrix Protein); Protein Bassoon; KIAA0434;

Function

Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:12812759).
After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (PubMed:19380881).
At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity).
Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy by associating with ATG5 (By similarity).
Mediates also synapse to nucleus communication leading to reconfiguration of gene expression by associating with the transcriptional corepressor CTBP1 and by subsequently reducing the size of its pool available for nuclear import (By similarity).

Biological Process

Chemical synaptic transmission Source: ProtInc
Presynapse to nucleus signaling pathway Source: Ensembl
Presynaptic active zone assembly Source: GO_Central
Protein localization to synapse Source: GO_Central
Regulation of synaptic vesicle cycle Source: Ensembl

Cellular Location

Cytoskeleton; Cytoplasm; Presynaptic active zone; Synaptic vesicle membrane. In retina, is localized in the outer plexiform layer at ribbon synapses formed by rods and cones but was absent from basal synaptic contacts formed by cones. In the retinal inner plexiform layer localized to conventional inhibitory GABAergic synapses, made by amacrine cells, but absent from the bipolar cell ribbon synapses.

PTM

Myristoylated. The N-terminal myristoylation is not sufficient for presynaptic localization (By similarity).

Montenegro-Venegas, C., Fienko, S., Anni, D., Pina-Fernández, E., Frischknecht, R., & Fejtova, A. (2021). Bassoon inhibits proteasome activity via interaction with PSMB4. Cellular and Molecular Life Sciences, 78(4), 1545-1563.

Ryl, M., Urbasik, A., Gierke, K., Babai, N., Joachimsthaler, A., Feigenspan, A., ... & Brandstätter, J. H. (2021). Genetic disruption of bassoon in two mutant mouse lines causes divergent retinal phenotypes. The FASEB Journal, 35(5), e21520.

Ghelani, T., Montenegro-Venegas, C., Fejtova, A., & Dresbach, T. (2021). Nanoscopical analysis reveals an orderly arrangement of the presynaptic scaffold protein Bassoon at the Golgi-apparatus. bioRxiv.

Montenegro-Venegas, C., Guhathakurta, D., Pina-Fernandez, E., Andres-Alonso, M., Plattner, F., Lazarevic, V., ... & Fejtova, A. (2021). Bassoon controls synaptic vesicle pools via regulation of presynaptic phosphorylation and cAMP homeostasis. bioRxiv.

Annamneedi, A., Del Angel, M., Gundelfinger, E. D., Stork, O., & Çalışkan, G. (2021). The Presynaptic Scaffold Protein Bassoon in Forebrain Excitatory Neurons Mediates Hippocampal Circuit Maturation: Potential Involvement of TrkB Signalling. International journal of molecular sciences, 22(15), 7944.

Hoffmann-Conaway, S., Brockmann, M. M., Schneider, K., Annamneedi, A., Rahman, K. A., Bruns, C., ... & Montenegro-Venegas, C. (2020). Parkin contributes to synaptic vesicle autophagy in Bassoon-deficient mice. Elife, 9, e56590.

Babai, N., Gierke, K., Müller, T., Regus‐Leidig, H., Brandstätter, J. H., & Feigenspan, A. (2019). Signal transmission at invaginating cone photoreceptor synaptic contacts following deletion of the presynaptic cytomatrix protein Bassoon in mouse retina. Acta Physiologica, 226(2), e13241.

Annamneedi, A., Caliskan, G., Müller, S., Montag, D., Budinger, E., Angenstein, F., ... & Stork, O. (2018). Ablation of the presynaptic organizer Bassoon in excitatory neurons retards dentate gyrus maturation and enhances learning performance. Brain Structure and Function, 223(7), 3423-3445.

Okerlund, N. D., Schneider, K., Leal-Ortiz, S., Montenegro-Venegas, C., Kim, S. A., Garner, L. C., ... & Garner, C. C. (2017). Bassoon controls presynaptic autophagy through Atg5. Neuron, 93(4), 897-913.

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Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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For research use only. Not intended for any clinical use.

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