Mouse Anti-ALB Recombinant Antibody (V2-363290) (CBMAB-S0173-CQ)

Name: Mouse Anti-ALB Recombinant Antibody (V2-363290)
SKU: CBMAB-S0173-CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ALB Recombinant Antibody (CBXS-4892) in IF

Double immunofluorescent images of Fpr2 (red) with albumin (green) in these cells. Gapdh was used as internal control. DAPI (blue) was used as nuclear counterstaining. ...View More

Lee, C., Kim, J., Han, J., Oh, D., Kim, M., Jeong, H., ... & Jung, Y. (2022). Formyl peptide receptor 2 determines sex-specific differences in the progression of nonalcoholic fatty liver disease and steatohepatitis. Nature Communications, 13(1), 578.

Mouse Anti-ALB Recombinant Antibody (CBXS-4892) in IF

Co-culture systems promote ALB expression of hepatocytes. a Representative confocal immunofluorescence images of ALB expression in hepatocytes after co-culturing for 7 days (magnification, ×40; scale bar, 50 μm). HUVECs are stained with DsRed; LO2 cells are stained with EGFP. Co-culture systems significantly enhanced ALB expression in hepatocytes compared with those in the monoculture. b Fluorescence intensity of ALB expression. c Fluorescence intensity of EGFP (LO2). For all groups, n = 3. *P < 0.05; **P < 0.01. ...View More

Wang, G., Zheng, Y., Wang, Y., Cai, Z., Liao, N., Liu, J., & Zhang, W. (2018). Co-culture system of hepatocytes and endothelial cells: two in vitro approaches for enhancing liver-specific functions of hepatocytes. Cytotechnology, 70, 1279-1290.

Mouse Anti-ALB Recombinant Antibody (CBXS-4892) in WB

Co-culture systems promote protein expression of ALB, CYP3A4 and HNF4α in hepatocytes. a Western blot analysis for ALB, CYP3A4, HNF4α and β-actin in hepatocytes after 7 days. ...View More

Mouse Anti-ALB Recombinant Antibody (CBXS-4892) in IF

Phase micrographs of induced hepatocytes during differentiation and proliferation stages and corresponding immunofluorescence micrographs of AFP and albumin, compared to primary hepatocytes and HepG2. Scale bars, 40 µm (top) and 100 µm (middle, bottom). Data are from donor 653. All n values represent the number of biological samples analyzed per condition. All error bars indicate ± s.d. ...View More

Levy, G., Bomze, D., Heinz, S., Ramachandran, S. D., Noerenberg, A., Cohen, M., ... & Nahmias, Y. (2015). Long-term culture and expansion of primary human hepatocytes. Nature biotechnology, 33(12), 1264-1271.

Mouse Anti-ALB Recombinant Antibody (CBXS-4892) in IF

Immunofluorescence analysis of CXCR4+ derivative cells. Cells were stained with antibodies against FOXA2 (I), HNF4A (II) or ALBUMIN (ALB) (III) (red). Nuclei were stained with Hoechst (blue). ...View More

Kopper, O., & Benvenisty, N. (2012). Stepwise differentiation of human embryonic stem cells into early endoderm derivatives and their molecular characterization. Stem cell research, 8(3), 335-345.

Mouse Anti-ALB Recombinant Antibody (CBXS-4892) in IF

Immunofluorescence double staining for CK7 (green) and albumin (red) and PAS staining in organoids derived from TSG-6-treated pHSCs cultured under E.M or D.M condition (X40, Scale bar: 20 μm). Representative images are shown. ...View More

Wang, S., Kim, J., Lee, C., Oh, D., Han, J., Kim, T. J., ... & Jung, Y. (2019). Tumor necrosis factor-inducible gene 6 reprograms hepatic stellate cells into stem-like cells, which ameliorates liver damage in mouse. Biomaterials, 219, 119375.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-363290

Application

WB, IHC-P, IF

Immunogen

ALB serum of human origin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	1:50-1:500
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Albumin

Introduction

This gene encodes the most abundant protein in human blood. This protein functions in the regulation of blood plasma colloid osmotic pressure and acts as a carrier protein for a wide range of endogenous molecules including hormones, fatty acids, and metabolites, as well as exogenous drugs. Additionally, this protein exhibits an esterase-like activity with broad substrate specificity. The encoded preproprotein is proteolytically processed to generate the mature protein. A peptide derived from this protein, EPI-X4, is an endogenous inhibitor of the CXCR4 chemokine receptor.

Entrez Gene ID

213

UniProt ID

P02768

Alternative Names

Albumin; Serum Albumin; PRO0883; PRO0903; PRO1341; HSA;

Function

Binds water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc> calcium> magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).

Biological Process

Cellular protein metabolic process Source: Reactome
Cellular response to starvation Source: UniProtKB
Heme biosynthetic process Source: Reactome
Heme catabolic process Source: Reactome
High-density lipoprotein particle remodeling Source: Reactome
Maintenance of mitochondrion location Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of programmed cell death Source: UniProtKB
Platelet degranulation Source: Reactome
Post-translational protein modification Source: Reactome
Receptor-mediated endocytosis Source: Reactome
Retina homeostasis Source: UniProtKB

Cellular Location

Secreted

Involvement in disease

Hyperthyroxinemia, familial dysalbuminemic (FDAH): A disorder characterized by abnormally elevated levels of total serum thyroxine (T4) in euthyroid patients. It is due to abnormal serum albumin that binds T4 with enhanced affinity.
Analbuminemia (ANALBA): A rare autosomal recessive disorder manifested by the presence of a very low amount of circulating serum albumin. Affected individuals manifest mild edema, hypotension, fatigue, and, occasionally, lower body lipodystrophy (mainly in adult females). The most common biochemical finding is hyperlipidemia, with a significant increase in the total and LDL cholesterol concentrations, but normal concentrations of HDL cholesterol and triglycerides.

PTM

Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylated by FAM20C in the extracellular medium.
Acetylated on Lys-223 by acetylsalicylic acid.

Fujioka-Kobayashi, M., Schaller, B., Mourão, C. F. D. A. B., Zhang, Y., Sculean, A., & Miron, R. J. (2021). Biological characterization of an injectable platelet-rich fibrin mixture consisting of autologous albumin gel and liquid platelet-rich fibrin (Alb-PRF). Platelets, 32(1), 74-81.

Hiraoka, R., Kuwahara, K., Wen, Y. C., Yen, T. H., Hiruta, Y., Cheng, C. M., & Citterio, D. (2020). Based Device for Naked Eye Urinary Albumin/Creatinine Ratio Evaluation. ACS sensors, 5(4), 1110-1118.

Bteich, M. (2019). An overview of albumin and alpha-1-acid glycoprotein main characteristics: highlighting the roles of amino acids in binding kinetics and molecular interactions. Heliyon, 5(11), e02879.

Miyamoto, T., Fujitani, M., Fukuyama, H., Hatanaka, S., Koizumi, Y., & Kawabata, A. (2019). The C-reactive protein/albumin ratio is useful for predicting short-term survival in cancer and noncancer patients. Journal of palliative medicine, 22(5), 532-537.

Mendes, R. D. S., Oliveira, M. V., Padilha, G. A., Rocha, N. N., Santos, C. L., Maia, L. A., ... & Silva, P. L. (2019). Effects of crystalloid, hyper-oncotic albumin, and iso-oncotic albumin on lung and kidney damage in experimental acute lung injury. Respiratory research, 20(1), 1-11.

Kudou, K., Saeki, H., Nakashima, Y., Kamori, T., Kawazoe, T., Haruta, Y., ... & Maehara, Y. (2019). C‐reactive protein/albumin ratio is a poor prognostic factor of esophagogastric junction and upper gastric cancer. Journal of gastroenterology and hepatology, 34(2), 355-363.

Li, S. Q., Jiang, Y. H., Lin, J., Zhang, J., Sun, F., Gao, Q. F., ... & Ying, H. Q. (2018). Albumin‐to‐fibrinogen ratio as a promising biomarker to predict clinical outcome of non‐small cell lung cancer individuals. Cancer medicine, 7(4), 1221-1231.

Oh, T. K., Song, I. A., & Lee, J. H. (2018). Clinical usefulness of C-reactive protein to albumin ratio in predicting 30-day mortality in critically ill patients: A retrospective analysis. Scientific reports, 8(1), 1-6.

Liu, Y., Chen, S., Zheng, C., Ding, M., Zhang, L., Wang, L., ... & Zhou, J. (2017). The prognostic value of the preoperative c-reactive protein/albumin ratio in ovarian cancer. BMC cancer, 17(1), 1-8.

Mao, M., Wei, X., Sheng, H., Chi, P., Liu, Y., Huang, X., ... & Liu, W. (2017). C‑reactive protein/albumin and neutrophil/lymphocyte ratios and their combination predict overall survival in patients with gastric cancer. Oncology letters, 14(6), 7417-7424.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

ALB Matched Antibody Pair (061) (CAT#: APMAB-061LY)

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For research use only. Not intended for any clinical use.

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