Mouse Anti-ALB Recombinant Antibody (V2-55272) (CBMAB-H0819-FY)

Name: Mouse Anti-ALB Recombinant Antibody (V2-55272)
SKU: CBMAB-H0819-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ALB Recombinant Antibody (CBFYH-0011) in IF

Confocal images of mouse liver tissue stained with the indicated antibodies and Hoechst. ...View More

Tocan, V., Hayase, J., Kamakura, S., Kohda, A., Ohga, S., Kohjima, M., & Sumimoto, H. (2021). Hepatocyte polarity establishment and apical lumen formation are organized by Par3, Cdc42, and aPKC in conjunction with Lgl. Journal of Biological Chemistry, 297(6).

Mouse Anti-ALB Recombinant Antibody (CBFYH-0011) in IHC

Cytomorphological and immunohistochemical characterization of mouse liver organoids as a model to test the toxicity induced by origami. ...View More

Palazzolo, S., Hadla, M., Russo Spena, C., Caligiuri, I., Rotondo, R., Adeel, M., ... & Rizzolio, F. (2019). An effective multi-stage liposomal DNA origami nanosystem for in vivo cancer therapy. Cancers, 11(12), 1997.

Mouse Anti-ALB Recombinant Antibody (CBFYH-0011) in WB

Western blot analysis for TSG101, RAB11, NCAM, calnexin and albumin in serum, plasma and CSF-derived exosome preparations isolated with the three kits. 35 μg of protein was used from each method, except for CSF ExoQ, were half of the protein concentration was loaded. SH: SH-SY5Y lysates. M: Molecular weight marker. ...View More

Soares Martins, T., Catita, J., Martins Rosa, I., AB da Cruz e Silva, O., & Henriques, A. G. (2018). Exosome isolation from distinct biofluids using precipitation and column-based approaches. PloS one, 13(6), e0198820.

Mouse Anti-ALB Recombinant Antibody (CBFYH-0011) in WB and IP

Enhanced binding between LPS and HMGB1 in sera of LPS-injected MCAO animals and inhibition of LPS-HMGB1 binding by blocking HMGB1.
Binding between HMGB1 and LPS was examined by co-immunoprecipitation-immunoblotting assay. a Serum was prepared from MCAO or PSI animals at 1 or 2 days post-MCAO and precipitated with anti-LPS antibody, and amounts of HMGB1 were determined by immunoblotting using anti-HMGB1 antibody. Amounts of HMGB1, LPS, or serum albumin before immunoprecipitations are presented as input controls. b Serum was prepared from MCAO or PSI animals at 2 days post-MCAO after pre-administrating HMGB1 A box (5 μg/kg) or HPep1 (5 μg/kg) at 21 h post-MCAO, and the same co-immunoprecipitation experiments were carried out. c, d Co-immunoprecipitation-immunoblotting assay was carried out using brain tissues prepared from the cortical penumbra of MCAO or PSI animals at 2 days post-MCAO. Results are presented as mean ± SEM (n = 4). Sham sham-operated rats. **p < 0.01 vs. treatment-naive MCAO controls, ##p < 0.01 vs. LPS-treated MCAO group. ...View More

Kim, I. D., Lee, H., Kim, S. W., Lee, H. K., Choi, J., Han, P. L., & Lee, J. K. (2018). Alarmin HMGB1 induces systemic and brain inflammatory exacerbation in post-stroke infection rat model. Cell Death & Disease, 9(4), 426.

Mouse Anti-ALB Recombinant Antibody (CBFYH-0011) in WB

Western blot of DBP and albumin in serial dilutions of plasma and central lymph. ...View More

Kongsbak, M., von Essen, M. R., Levring, T. B., Schjerling, P., Woetmann, A., Ødum, N., ... & Geisler, C. (2014). Vitamin D-binding protein controls T cell responses to vitamin D. BMC immunology, 15, 1-13.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-55272

Application

ICC, IF, WB, IP , ELISA, IHC-P

Immunogen

Amino acids 559-591 near the C-terminus of serum albumin of mouse origin.

Host Species

Mouse

Specificity

Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:30-1:3,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Albumin

Introduction

This gene encodes the most abundant protein in human blood. This protein functions in the regulation of blood plasma colloid osmotic pressure and acts as a carrier protein for a wide range of endogenous molecules including hormones, fatty acids, and metabolites, as well as exogenous drugs. Additionally, this protein exhibits an esterase-like activity with broad substrate specificity. The encoded preproprotein is proteolytically processed to generate the mature protein. A peptide derived from this protein, EPI-X4, is an endogenous inhibitor of the CXCR4 chemokine receptor.

Entrez Gene ID

213

UniProt ID

P02768

Alternative Names

Albumin; Serum Albumin; PRO0883; PRO0903; PRO1341; HSA

Function

Binds water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs (Probable). Its main function is the regulation of the colloidal osmotic pressure of blood (Probable). Major zinc transporter in plasma, typically binds about 80% of all plasma zinc (PubMed:19021548). Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma (By similarity). Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner (By similarity). The shared binding site between zinc and calcium at residue Asp-273 suggests a crosstalk between zinc and calcium transport in the blood (By similarity). The rank order of affinity is zinc> calcium> magnesium (By similarity). Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli (PubMed:6234017). Does not prevent iron uptake by the bacterial siderophore aerobactin (PubMed:6234017).

Biological Process

Cellular protein metabolic process Source: Reactome
Cellular response to starvation Source: UniProtKB
Heme biosynthetic process Source: Reactome
Heme catabolic process Source: Reactome
High-density lipoprotein particle remodeling Source: Reactome
Maintenance of mitochondrion location Source: UniProtKB
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of programmed cell death Source: UniProtKB
Platelet degranulation Source: Reactome
Post-translational protein modification Source: Reactome
Receptor-mediated endocytosis Source: Reactome
Retina homeostasis Source: UniProtKB

Cellular Location

Secreted

Involvement in disease

Hyperthyroxinemia, familial dysalbuminemic (FDAH): A disorder characterized by abnormally elevated levels of total serum thyroxine (T4) in euthyroid patients. It is due to abnormal serum albumin that binds T4 with enhanced affinity.
Analbuminemia (ANALBA): A rare autosomal recessive disorder manifested by the presence of a very low amount of circulating serum albumin. Affected individuals manifest mild edema, hypotension, fatigue, and, occasionally, lower body lipodystrophy (mainly in adult females). The most common biochemical finding is hyperlipidemia, with a significant increase in the total and LDL cholesterol concentrations, but normal concentrations of HDL cholesterol and triglycerides.

PTM

Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylated by FAM20C in the extracellular medium.
Acetylated on Lys-223 by acetylsalicylic acid.

Fujioka-Kobayashi, M., Schaller, B., Mourão, C. F. D. A. B., Zhang, Y., Sculean, A., & Miron, R. J. (2021). Biological characterization of an injectable platelet-rich fibrin mixture consisting of autologous albumin gel and liquid platelet-rich fibrin (Alb-PRF). Platelets, 32(1), 74-81.

Hiraoka, R., Kuwahara, K., Wen, Y. C., Yen, T. H., Hiruta, Y., Cheng, C. M., & Citterio, D. (2020). Based Device for Naked Eye Urinary Albumin/Creatinine Ratio Evaluation. ACS sensors, 5(4), 1110-1118.

Bteich, M. (2019). An overview of albumin and alpha-1-acid glycoprotein main characteristics: highlighting the roles of amino acids in binding kinetics and molecular interactions. Heliyon, 5(11), e02879.

Miyamoto, T., Fujitani, M., Fukuyama, H., Hatanaka, S., Koizumi, Y., & Kawabata, A. (2019). The C-reactive protein/albumin ratio is useful for predicting short-term survival in cancer and noncancer patients. Journal of palliative medicine, 22(5), 532-537.

Mendes, R. D. S., Oliveira, M. V., Padilha, G. A., Rocha, N. N., Santos, C. L., Maia, L. A., ... & Silva, P. L. (2019). Effects of crystalloid, hyper-oncotic albumin, and iso-oncotic albumin on lung and kidney damage in experimental acute lung injury. Respiratory research, 20(1), 1-11.

Kudou, K., Saeki, H., Nakashima, Y., Kamori, T., Kawazoe, T., Haruta, Y., ... & Maehara, Y. (2019). C‐reactive protein/albumin ratio is a poor prognostic factor of esophagogastric junction and upper gastric cancer. Journal of gastroenterology and hepatology, 34(2), 355-363.

Li, S. Q., Jiang, Y. H., Lin, J., Zhang, J., Sun, F., Gao, Q. F., ... & Ying, H. Q. (2018). Albumin‐to‐fibrinogen ratio as a promising biomarker to predict clinical outcome of non‐small cell lung cancer individuals. Cancer medicine, 7(4), 1221-1231.

Oh, T. K., Song, I. A., & Lee, J. H. (2018). Clinical usefulness of C-reactive protein to albumin ratio in predicting 30-day mortality in critically ill patients: A retrospective analysis. Scientific reports, 8(1), 1-6.

Liu, Y., Chen, S., Zheng, C., Ding, M., Zhang, L., Wang, L., ... & Zhou, J. (2017). The prognostic value of the preoperative c-reactive protein/albumin ratio in ovarian cancer. BMC cancer, 17(1), 1-8.

Mao, M., Wei, X., Sheng, H., Chi, P., Liu, Y., Huang, X., ... & Liu, W. (2017). C‑reactive protein/albumin and neutrophil/lymphocyte ratios and their combination predict overall survival in patients with gastric cancer. Oncology letters, 14(6), 7417-7424.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

ALB Matched Antibody Pair (061) (CAT#: APMAB-061LY)

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For research use only. Not intended for any clinical use.

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