Mouse Anti-ASB9 Recombinant Antibody (1D8) (CBMAB-A0529-LY)

The product is antibody recognizes ASB9. The antibody 1D8 immunoassay techniques such as: WB, ELISA.
See all ASB9 antibodies

Published Data

Mouse Anti-ASB9 Recombinant Antibody (1D8) CBMAB-A0529-LY in IF

A confocal laser scanning microscope was used to visualise the structure of mitochondria with the aid of DSRed2-Mito. ...View More

Kwon, S., Kim, D., Rhee, J. W., Park, J. A., Kim, D. W., Kim, D. S., ... & Kwon, H. J. (2010). ASB9 interacts with ubiquitous mitochondrial creatine kinase and inhibits mitochondrial function. BMC biology, 8, 1-22.

Mouse Anti-ASB9 Recombinant Antibody (1D8) CBMAB-A0529-LY in Immunoblot

The lysates obtained from HEK293 cells stably transfected with Myc-tagged ASB9 and ASB9ΔSOCS expression vectors were immunoprecipitated with an anti-Myc antibody and subjected to immunoblotting with anti-Myc, anti-CKB, anti-uMtCK, and anti-elongin B antibodies. ...View More

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1D8

Antibody Isotype

IgG1, κ

Application

WB, ELISA, IHC-P

Basic Information

Immunogen

ASB9 (AAH01244, 1 a.a. ~ 252 a.a) full-length recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC	3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Ankyrin Repeat And SOCS Box Containing 9

Entrez Gene ID

140462

UniProt ID

Q96DX5

Alternative Names

DKFZp564L0862; FLJ20636; MGC4954

Function

Substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes at least two forms of creatine kinase, CKB and CKMT1A.

Biological Process

Intracellular signal transduction Source: InterPro
Positive regulation of protein catabolic process Source: MGI
Post-translational protein modification Source: Reactome
Protein ubiquitination Source: MGI

Cellular Location

Mitochondrion

References

Nosratpour, S., & Ndiaye, K. (2021). Ankyrin‐repeat and SOCS box‐containing protein 9 (ASB9) regulates ovarian granulosa cells function and MAPK signaling. Molecular Reproduction and Development.

Lumpkin, R. J., Baker, R. W., Leschziner, A. E., & Komives, E. A. (2020). Structure and dynamics of the ASB9 CUL-RING E3 ligase. Nature communications, 11(1), 1-11.

Benoit, G., Warma, A., Lussier, J. G., & Ndiaye, K. (2019). Gonadotropin regulation of ankyrin-repeat and SOCS-box protein 9 (ASB9) in ovarian follicles and identification of binding partners. PloS one, 14(2), e0212571.

Benoit, G. (2019). Étude de l’expression et de la fonction du gène Ankyrin-repeat and SOCS-Box protein 9 (ASB9) dans le follicule ovulatoire bovin.

Benoit, G., Lussier, J. G., & Ndiaye, K. (2017). Regulation of Ankyrin‐repeat and SOCS‐box protein 9 (ASB9) in ovarian follicles and identification of binding partners. The FASEB Journal, 31, 601-12.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Antibody Pairs

ASB9 Matched Antibody Pair (108)

ASB9 Matched Antibody Pair (109)

Related Products

ASB9 Matched Antibody Pair (108)

ASB9 Matched Antibody Pair (109)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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