Mouse Anti-ALOX5 Recombinant Antibody (33) (CBMAB-1890CQ)
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Mouse Anti-ALOX5 Recombinant Antibody (33) in WB
LAMA84 cells were cultured in presence of the indicated concentration of free PM and BPTES for 24 h, then cells were harvested for western blotting analysis with anti BCR-ABL, c-MYC, EZH2, ALOX5, ALOX15, HOXA9, HSP90, P21 and P27 antibodies. GAPDH served as the total protein loading control.
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Qiu, Q., Feng, Y., Zhu, Z., Li, N., Zheng, L., Sun, Y., ... & Hu, Y. (2023). HDAC I/IIb selective inhibitor Purinostat Mesylate combined with GLS1 inhibition effectively eliminates CML stem cells. Bioactive Materials, 21, 483-498.
Mouse Anti-ALOX5 Recombinant Antibody (33) in WB
Western blot analysis of ALOX5 at 72 h in control macrophages (n = 11) or those polarized to a pro-inflammatory (n = 11) or anti-inflammatory (n = 11) phenotype, normalized to β-actin and shown relative to control macrophages.
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Günes Günsel, G., Conlon, T. M., Jeridi, A., Kim, R., Ertüz, Z., Lang, N. J., ... & Yildirim, A. O. (2022). The arginine methyltransferase PRMT7 promotes extravasation of monocytes resulting in tissue injury in COPD. Nature communications, 13(1), 1303.
Mouse Anti-ALOX5 Recombinant Antibody (33) in WB
Western blotting results revealing that zileuton dose-dependently decreases 5-LO protein levels in CD34+ cells from a patient with PV. (A) Western blotting for the detection of 5-LO protein expression in CD34+ cells from 1 patient with PV and 1 normal control after treatment with increasing concentrations of zileuton. (B) Ratios of 5-LO/β-actin were calculated. Zileuton treatment dose-dependently decreased 5-LO protein expression compared with the control treatment in CD34+ cells from a patient with PV. All values are shown as medians and interquartile ranges of data from experiments performed in triplicate. The Friedman test was used to determine significance and a Nemenyi post hoc test was subsequently used for pairwise comparisons between groups.
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Chen, Y., Zhao, H., Luo, J., Liao, Y., Tan, K., & Hu, G. (2021). A drug targeting 5‑lipoxygenase enhances the activity of a JAK2 inhibitor in CD34+ bone marrow cells from patients with JAK2V617F‑positive polycythemia vera in vitro. Oncology Letters, 21(5), 1-10.
Mouse Anti-ALOX5 Recombinant Antibody (33) in IHC
Immunolabeling and quantification of COX2 and F4/80 + cells at 7 and 21 days post tooth extraction in WT and 5LOKO mice, treated or not with ZL. Aged 129 Sv WT female mice received IP injections of 0.9% saline solution (C) or 250 μg/Kg (ZL groups) once a week, and upper right incisor were extracted at 4 week of each treatment. Mice were euthanized for maxillary bones removal after 7 days and 21 days post tooth extraction. (A) Representative transversal sections from the central area of alveolar sockets stained for COX2 + cells (A) and F4/0 + cells (B). Quantitative and comparative analysis of COX2 + cells (A') and F4/80 + cells (B') among groups. Scale bar: 50 µm. Quantitative results are presented with Box and whiskers (Min to Max). Symbol * indicates a statistically significant difference between groups (p < 0.05).
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Mahmoud, R. H., Biguetti, C. C., Simionato, G. B., Custódio, I. C., Silva, R. B., Duarte, M. A., ... & Matsumoto, M. A. (2021). Alveolar socket healing in 5-lipoxygenase knockout aged female mice treated or not with high dose of zoledronic acid. Scientific Reports, 11(1), 19535.
Mouse Anti-ALOX5 Recombinant Antibody (33) in IHC
Histopathological characterization of bone healing 7 and 14 days post-surgery: Male and female, young (3 months old), and aged (18 months old) 129/SvEv WT and 5LOKO mice underwent our surgical injury model, and bone specimens were evaluated at 7 and 14 days post-surgery. (A) Representative images from young and aged animals (WT and 5LOKO) stained with H&E and Goldner's Trichrome/Alcian Blue. Images were captured at 10× magnification. (B) H&E representative images from aged animals at 14 days were captured at 100× magnification.
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Biguetti, C. C., Couto, M. C. R., Silva, A. C. R., Shindo, J. V. T. C., Rosa, V. M., Shinohara, A. L., ... & Matsumoto, M. A. (2020). New surgical model for bone-muscle injury reveals age and gender-related healing patterns in the 5 lipoxygenase (5LO) knockout mouse. Frontiers in Endocrinology, 11, 484.
Mouse Anti-ALOX5 Recombinant Antibody (33) in WB
hVSMCs were pre-treated with zileuton (0 to 10 μM) for 1 h or transfected with 5-LO siRNA (200 nM) for 48 h, and then stimulated with HMGB1 (100 ng/ ml) for 24 h.
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Jang, E. J., Baek, S. E., Kim, E. J., Park, S. Y., & Kim, C. D. (2019). HMGB1 enhances AGE-mediated VSMC proliferation via an increase in 5-LO-linked RAGE expression. Vascular Pharmacology, 118, 106559.
Datasheet
Target
References
Q & As
Review & reward
Protocols
Associated Products
Basic Information
Host Animal
Mouse
Clone
33
Application
IF, WB, IP
Immunogen
C-terminus portion of 5-LO of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
| Application | Note |
| WB | 1:100-1:1,000 |
| IP | 1-2 µg per 100-500 µg of total protein (1 ml of cell lysate) |
| IF(ICC) | 1:50-1:500 |
Formulations & Storage [For reference only, actual COA shall prevail!]
Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation
Target
Full Name
Arachidonate 5-Lipoxygenase
Introduction
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Diseases associated with ALOX5 include Asthma and Arthus Reaction. Among its related pathways are PEDF Induced Signaling and Cytokine Signaling in Immune system. Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.
Entrez Gene ID
UniProt ID
Alternative Names
arachidonate 5-lipoxygenase; 5-LO; 5LPG; LOG5; 5-LOX; arachidonate 5-lipoxygenase; LOX-5; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic acid 5-lipoxygenase; leukotriene A4 synthase; EC 1.13.11.34
Function
Catalyzes the oxygenation of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to 5-hydroperoxyeicosatetraenoate (5-HPETE) followed by the dehydration to 5,6- epoxyeicosatetraenoate (Leukotriene A4/LTA4), the first two steps in the biosynthesis of leukotrienes, which are potent mediators of inflammation (PubMed:8631361, PubMed:21233389, PubMed:22516296, PubMed:24282679, PubMed:19022417, PubMed:23246375, PubMed:8615788, PubMed:24893149, PubMed:31664810). Also catalyzes the oxygenation of arachidonate into 8-hydroperoxyicosatetraenoate (8-HPETE) and 12-hydroperoxyicosatetraenoate (12-HPETE) (PubMed:23246375). Displays lipoxin synthase activity being able to convert (15S)-HETE into a conjugate tetraene (PubMed:31664810). Although arachidonate is the preferred substrate, this enzyme can also metabolize oxidized fatty acids derived from arachidonate such as (15S)-HETE, eicosapentaenoate (EPA) such as (18R)- and (18S)-HEPE or docosahexaenoate (DHA) which lead to the formation of specialized pro-resolving mediators (SPM) lipoxin and resolvins E and D respectively, therefore it participates in anti-inflammatory responses (PubMed:21206090, PubMed:31664810, PubMed:8615788, PubMed:17114001, PubMed:32404334). Oxidation of DHA directly inhibits endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator-activated receptor gamma (PPARgamma) (By similarity). It does not catalyze the oxygenation of linoleic acid and does not convert (5S)-HETE to lipoxin isomers (PubMed:31664810). In addition to inflammatory processes, it participates in dendritic cell migration, wound healing through an antioxidant mechanism based on heme oxygenase-1 (HO-1) regulation expression, monocyte adhesion to the endothelium via ITGAM expression on monocytes (By similarity). Moreover, it helps establish an adaptive humoral immunity by regulating primary resting B cells and follicular helper T cells and participates in the CD40-induced production of reactive oxygen species (ROS) after CD40 ligation in B cells through interaction with PIK3R1 that bridges ALOX5 with CD40 (PubMed:21200133). Also may play a role in glucose homeostasis, regulation of insulin secretion and palmitic acid-induced insulin resistance via AMPK (By similarity). Can regulate bone mineralization and fat cell differentiation increases in induced pluripotent stem cells (By similarity).
Biological Process
Arachidonic acid metabolic process Source: GO_Central
Cytokine-mediated signaling pathway Source: Reactome
Dendritic cell migration Source: UniProtKB
Glucose homeostasis Source: UniProtKB
Hepoxilin biosynthetic process Source: GO_Central
Humoral immune response Source: UniProtKB
Interleukin-18-mediated signaling pathway Source: Reactome
Leukocyte chemotaxis involved in inflammatory response Source: UniProtKB
Leukocyte migration involved in inflammatory response Source: UniProtKB
Leukotriene A4 biosynthetic process Source: UniProtKB
Leukotriene biosynthetic process Source: UniProtKB
Leukotriene metabolic process Source: Reactome
Leukotriene production involved in inflammatory response Source: Ensembl
Linoleic acid metabolic process Source: GO_Central
Lipid oxidation Source: GO_Central
Lipoxin biosynthetic process Source: UniProtKB
Lipoxygenase pathway Source: GO_Central
Long-chain fatty acid biosynthetic process Source: Reactome
Negative regulation of angiogenesis Source: UniProtKB
Negative regulation of endothelial cell proliferation Source: UniProtKB
Negative regulation of inflammatory response Source: UniProtKB
Negative regulation of response to endoplasmic reticulum stress Source: UniProtKB
Negative regulation of sprouting angiogenesis Source: UniProtKB
Negative regulation of vascular wound healing Source: UniProtKB
Negative regulation of wound healing Source: UniProtKB
Neutrophil degranulation Source: Reactome
Positive regulation of bone mineralization Source: UniProtKB
Positive regulation of leukocyte adhesion to arterial endothelial cell Source: UniProtKB
Regulation of cellular response to oxidative stress Source: UniProtKB
Regulation of cytokine production involved in inflammatory response Source: UniProtKB
Regulation of fat cell differentiation Source: UniProtKB
Regulation of inflammatory response Source: UniProtKB
Regulation of inflammatory response to wounding Source: UniProtKB
Regulation of insulin secretion Source: UniProtKB
Regulation of reactive oxygen species biosynthetic process
Cytokine-mediated signaling pathway Source: Reactome
Dendritic cell migration Source: UniProtKB
Glucose homeostasis Source: UniProtKB
Hepoxilin biosynthetic process Source: GO_Central
Humoral immune response Source: UniProtKB
Interleukin-18-mediated signaling pathway Source: Reactome
Leukocyte chemotaxis involved in inflammatory response Source: UniProtKB
Leukocyte migration involved in inflammatory response Source: UniProtKB
Leukotriene A4 biosynthetic process Source: UniProtKB
Leukotriene biosynthetic process Source: UniProtKB
Leukotriene metabolic process Source: Reactome
Leukotriene production involved in inflammatory response Source: Ensembl
Linoleic acid metabolic process Source: GO_Central
Lipid oxidation Source: GO_Central
Lipoxin biosynthetic process Source: UniProtKB
Lipoxygenase pathway Source: GO_Central
Long-chain fatty acid biosynthetic process Source: Reactome
Negative regulation of angiogenesis Source: UniProtKB
Negative regulation of endothelial cell proliferation Source: UniProtKB
Negative regulation of inflammatory response Source: UniProtKB
Negative regulation of response to endoplasmic reticulum stress Source: UniProtKB
Negative regulation of sprouting angiogenesis Source: UniProtKB
Negative regulation of vascular wound healing Source: UniProtKB
Negative regulation of wound healing Source: UniProtKB
Neutrophil degranulation Source: Reactome
Positive regulation of bone mineralization Source: UniProtKB
Positive regulation of leukocyte adhesion to arterial endothelial cell Source: UniProtKB
Regulation of cellular response to oxidative stress Source: UniProtKB
Regulation of cytokine production involved in inflammatory response Source: UniProtKB
Regulation of fat cell differentiation Source: UniProtKB
Regulation of inflammatory response Source: UniProtKB
Regulation of inflammatory response to wounding Source: UniProtKB
Regulation of insulin secretion Source: UniProtKB
Regulation of reactive oxygen species biosynthetic process
Cellular Location
Cytoplasm; Cytosol; Nucleus matrix; Nucleus membrane; Nucleus envelope; Nucleus intermembrane space; Perinuclear region. Shuttles between cytoplasm and nucleus (PubMed:19233132). Found exclusively in the nucleus, when phosphorylated on Ser-272 (PubMed:18978352). Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association (PubMed:19233132, PubMed:3118366, PubMed:8245774, PubMed:16275640).
PTM
Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid (PubMed:11844797, PubMed:18978352). Phosphorylation on Ser-524 by PKA has an inhibitory effect (PubMed:15280375). Phosphorylation on Ser-272 prevents export from the nucleus (PubMed:11844797, PubMed:18978352). Phosphorylation at Ser-524 is stimulated by 8-bromo-3',5'-cyclic AMP or prostaglandin E2 (PubMed:26210919).
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols
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For research use only. Not intended for any clinical use.
Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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