What are the validated applications for this ABIN2 antibody?

This Mouse Anti-ABIN2 Recombinant Antibody (CBMAB-A0349-YC) has been thoroughly validated for several common laboratory applications, including Western Blot (WB), Immunofluorescence (IF), Enzyme-Linked Immunosorbent Assay (ELISA), and Immunoprecipitation (IP). This broad range of validated applications makes it a highly versatile tool for researchers studying ABIN2.

Can this antibody detect ABIN2 in different species, or is it specifically reactive with human ABIN2?

This antibody is a mouse recombinant antibody. While it is often developed and validated for human samples due to the high conservation of many target proteins, its reactivity with ABIN2 from other species (e.g., mouse, rat) would depend on the degree of sequence homology within the epitope region. Always refer to the product datasheet provided by Creative Biolabs for the most accurate and up-to-date information on validated species reactivity. If your specific species of interest is not listed, it's advisable to perform a preliminary cross-reactivity test in your own experimental system.

Mouse Anti-ABIN2 Recombinant Antibody (V2-179106) (CBMAB-A0349-YC)

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Published Data

Mouse Anti-ABIN2 Recombinant Antibody (H-8) (CBMAB-A0349-YC) in WB

TNIP2 expression in MODS. The level of TNIP2 in MODS was detected using western blot analysis.Protein level of TNIP2 in the blood of MODS patients. ...View More

Gong, H., Sheng, X., Xue, J., & Zhu, D. (2019). Expression and role of TNIP2 in multiple organ dysfunction syndrome following severe trauma. Molecular Medicine Reports, 19(4), 2906-2912.

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179106

Application

WB, IP, IF, ELISA

Immunogen

Raised against amino acids 1-300 mapping at the N-terminus of Abin-2 of human origin.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

TNFAIP3 interacting protein 2

Introduction

ABIN2 is a protein which acts as an inhibitor of NFkappaB activation. The encoded protein is also involved in MAP/ERK signaling pathway in specific cell types. It may be involved in apoptosis of endothelial cells. A pseudogene related to this gene is loca

Entrez Gene ID

79155

UniProt ID

Q8NFZ5

Alternative Names

KLIP; ABIN2; FLIP1; TNFAIP3-interacting protein 2; A20-binding inhibitor of NF-kappaB activation-2; fetal liver LKB1-interacting protein

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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