What applications has this antibody been validated for by Creative Biolabs?

Based on the product information available, the Mouse Anti-DISP2 Monoclonal Antibody (F66A4B1) (CAT: CBMAB-1112CQ) is indicated for research use only and not for clinical diagnosis. Typical validation for research antibodies of this nature includes Western Blot (WB) and potentially Immunohistochemistry (IHC) or Immunofluorescence (IF). Please refer to the product datasheet provided by Creative Biolabs for the most up-to-date and specific validated applications and recommended working dilutions.

Is a recommended working dilution provided for specific applications like Western Blot or Immunofluorescence?

Yes, Creative Biolabs typically provides recommended starting dilutions for validated applications on the product datasheet. These recommendations are based on their internal quality control testing. However, optimal working dilutions can vary depending on the experimental conditions, sample type, and detection method used. It is always advisable to perform a dilution series to determine the optimal antibody concentration for your specific experiment.

Mouse Anti-DISP2 Monoclonal Antibody (F66A4B1) (CBMAB-1112CQ)

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Published Data

Mouse Anti-DISP2 Monoclonal Antibody (F66A4B1) (CBMAB-1112CQ-1) in IHC

Cell-specific localization of DISP2 in the adult human brain. Immunohistochemical staining for DISP2 (red, a, b, c, d) and the neuronal marker PGP9.5 (brown, a, b) or the glial marker S100 (brown, c, d). Nuclei were counterstained with hematoxylin (blue). Costaining of DISP2 with PGP9.5 shows colocalization in neuronal somata and processes (b). Immunofluorescence microscopy of the same section (b') for DISP2 (red) confirmed colocalization of DISP2 with PGP9.5, excluding the cell nuclei. Costaining of DISP2 with the glial marker S100 on consecutive sections (d) reveals no colocalization of DISP2 with S100-positive glial cells (marked by arrowheads), confirmed by immunofluorescence microscopy of the same section (d') (scale bar in a=200 µm, b=100 µm; n=5) ...View More

Heimke, M., Richter, F., Heinze, T., Kunke, M., Wedel, T., Böttner, M., ... & Cossais, F. (2023). Localization Pattern of Dispatched Homolog 2 (DISP2) in the Central and Enteric Nervous System. Journal of Molecular Neuroscience, 73(7), 539-548.

Mouse Anti-DISP2 Monoclonal Antibody (F66A4B1) (CBMAB-1112CQ-2) in IHC

DISP2 localization pattern in the adult rat central nervous system. Immunohistochemical staining for DISP2 was performed on frontal sections of adult rat brain. Magnifications for the motor cortex (b, b') and the dentate gyrus of the hippocampus (c, c') show accumulation of DISP2 (red, b1, c1) in PGP9.5- (green, b2, c2) positive neuronal cell somata and processes. Costaining with the glial marker S100 (green, b'2, c'2, d'2) on consecutive sections revealed no colocalization with DISP2 but strong DISP2 immunoreactivity at the apical pole of S100-positive ependymal cells (d). Nuclei were counterstained with Hoechst (blue), and corresponding merge images are shown (b3, b'3, c3, c'3, d3, d'3) (scale bar in a=2000 µm, b, c, d=50 µm; n≥3). ...View More

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

F66A4B1

Application

FC, IHC

Immunogen

Synthetic peptide (aa731-750) coupled to KLH.

Host Species

Mouse

Specificity

Human, Mouse

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IHC	2-5 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

dispatched RND transporter family member 2

Introduction

This gene is one of two human homologs of a segment-polarity gene known as dispatched identified in Drosophila. The product of this gene may be required for normal Hedgehog (Hh) signaling during embryonic pattern formation. Among its related pathways are Hedgehog Pathway and CDK-mediated phosphorylation and removal of Cdc6.

Entrez Gene ID

85455

UniProt ID

A7MBM2

Alternative Names

s DISPB; C15orf36; HsT16908; LINC00594

Biological Process

Smoothened signaling pathway Source: UniProtKB

Cellular Location

Membrane

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Related Products

Mouse Anti-DISP2 Recombinant Antibody (CBYCD-283) (CAT#: V2LY-0425-LY375)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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