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Rabbit Anti-EPHA3 Monoclonal Antibody (EPR3434(N)) (CBMAB-1164-YC)

Provided herein is a rabbit monoclonal antibody against Human EPHA3. The antibody, clone EPR3434(N), can be used for immunoassay techniques, such as WB.
See all EPHA3 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse
Clone
EPR3434(N)
Antibody Isotype
IgG
Application
WB

Basic Information

Immunogen
Synthetic peptide within Human Eph receptor A3 aa 550-650 (phospho Y596 + Y602).
Specificity
Human, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
EPH Receptor A3
Introduction
EPHA3 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 binds ephrin-A ligands.
Entrez Gene ID
Human2042
Mouse13837
UniProt ID
HumanP29320
MouseP29319
Alternative Names
EK4; ETK; HEK; ETK1; HEK4; TYRO4
Research Area
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.
Biological Process
Axon guidance Source: GO_Central
Cell adhesion Source: UniProtKB-KW
Cell migration Source: UniProtKB
Cellular response to retinoic acid Source: BHF-UCL
Ephrin receptor signaling pathway Source: UniProtKB
Fasciculation of motor neuron axon Source: UniProtKB
Fasciculation of sensory neuron axon Source: UniProtKB
Negative regulation of endocytosis Source: UniProtKB
Peptidyl-tyrosine phosphorylation Source: UniProtKB
Positive regulation of kinase activity Source: GO_Central
Positive regulation of neuron projection development Source: BHF-UCL
Positive regulation of protein localization to plasma membrane Source: UniProtKB
Regulation of actin cytoskeleton organization Source: UniProtKB
Regulation of epithelial to mesenchymal transition Source: UniProtKB
Regulation of focal adhesion assembly Source: UniProtKB
Regulation of GTPase activity Source: UniProtKB
Regulation of microtubule cytoskeleton organization Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Cellular Location
Isoform 1: Cell membrane
Isoform 2: Secreted
Involvement in disease
Colorectal cancer (CRC):
A complex disease characterized by malignant lesions arising from the inner wall of the large intestine (the colon) and the rectum. Genetic alterations are often associated with progression from premalignant lesion (adenoma) to invasive adenocarcinoma. Risk factors for cancer of the colon and rectum include colon polyps, long-standing ulcerative colitis, and genetic family history.
Topology
Extracellular: 21-541
Helical: 542-565
Cytoplasmic: 566-983
PTM
Autophosphorylates upon activation by EFNA5. Phosphorylation on Tyr-602 mediates interaction with NCK1. Dephosphorylated by PTPN1.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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