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Mouse Anti-ETFA Recombinant Antibody (2A5) (CBMAB-A2721-LY)

The product is antibody recognizes ETFA. The antibody 2A5 immunoassay techniques such as: sELISA, ELISA.
See all ETFA antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2A5
Antibody Isotype
IgG2a, κ
Application
sELISA, ELISA

Basic Information

Immunogen
ETFA (AAH15526, 1 a.a. ~ 333 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
electron-transfer-flavoprotein, alpha polypeptide
Introduction
ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Entrez Gene ID
2108
UniProt ID
P13804
Alternative Names
EMA; GA2; MADD
Research Area
Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase (PubMed:27499296, PubMed:15159392, PubMed:15975918, PubMed:9334218, PubMed:10356313).

It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (PubMed:9334218).

Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism (PubMed:12815589, PubMed:1882842, PubMed:1430199).
Biological Process
Fatty acid beta-oxidation using acyl-CoA dehydrogenase Source: UniProtKB
Cellular Location
Mitochondrion matrix
Involvement in disease
Glutaric aciduria 2A (GA2A):
An autosomal recessively inherited disorder of fatty acid, amino acid, and choline metabolism. It is characterized by multiple acyl-CoA dehydrogenase deficiencies resulting in large excretion not only of glutaric acid, but also of lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids.
PTM
The N-terminus is blocked.

Yan, Y., Xu, Y., Yang, X., Li, Z., Niu, K., Liu, C., ... & Wu, W. (2022). Electron Transfer Flavoprotein (ETF) α Controls Blood Vessel Development by Regulating Endothelial Mitochondrial Bioenergetics and Oxygen Consumption. Oxidative medicine and cellular longevity, 2022.

Salerno, K. M., Domenico, J., Le, N. Q., Stiles, C. D., Solov’yov, I. A., & Martino, C. F. (2022). Long-Time Oxygen Localization in Electron Transfer Flavoprotein. Journal of Chemical Information and Modeling, 62(17), 4191-4199.

Park, K. H., Gooz, M., Ye, Z. W., Zhang, J., Beeson, G. C., Rockey, D. C., & Kim, S. H. (2021). Flavin Adenine Dinucleotide Depletion Caused by electron transfer flavoprotein subunit alpha Haploinsufficiency Leads to Hepatic Steatosis and Injury in Zebrafish. Hepatology communications, 5(6), 976-991.

Henriques, B. J., Olsen, R. K. J., Gomes, C. M., & Bross, P. (2021). Electron transfer flavoprotein and its role in mitochondrial energy metabolism in health and disease. Gene, 776, 145407.

Sucharitakul, J., Buckel, W., & Chaiyen, P. (2021). Rapid kinetics reveal surprising flavin chemistry in bifurcating electron transfer flavoprotein from Acidaminococcus fermentans. Journal of Biological Chemistry, 296.

Ou, M., Zhu, L., Zhang, Y., Zhang, Y., Zhou, J., Zhang, Y., ... & Wang, W. (2020). A novel electron transfer flavoprotein dehydrogenase (ETFDH) gene mutation identified in a newborn with glutaric acidemia type II: a case report of a Chinese family. BMC Medical Genetics, 21(1), 1-7.

Vogt, M. S., Schühle, K., Kölzer, S., Peschke, P., Chowdhury, N. P., Kleinsorge, D., ... & Heider, J. (2019). Structural and functional characterization of an electron transfer flavoprotein involved in toluene degradation in strictly anaerobic bacteria. Journal of Bacteriology, 201(21), e00326-19.

Yu, H. M., Yao, X. D., Zhang, R. M., Zhuang, H. F., Wang, P. W., & Li, Y. Z. (2019). Repression of let‐7b‐5p prevents the development of multifidus muscle dysfunction by promoting vitamin D accumulation via upregulation of electron transfer flavoprotein alpha subunit in a rat model of multifidus muscle injury. Journal of Cellular Biochemistry, 120(5), 7458-7473.

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For research use only. Not intended for any clinical use.

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