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Mouse Anti-FBXO9 Recombinant Antibody (CBXF-2911) (CBMAB-F3618-CQ)

This product is a mouse antibody that recognizes FBXO9. The antibody CBXF-2911 can be used for immunoassay techniques such as: ELISA, IHC.
See all FBXO9 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBXF-2911
Antibody Isotype
IgG2a, κ
Application
ELISA, IHC

Basic Information

Immunogen
Partial recombinant corresponding to aa338-448 from human FBXO9 (NP_036479) with GST tag
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
F-box protein 9
Introduction
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates at least 3 transcript variants diverging at the 5' terminus.
Entrez Gene ID
26268
UniProt ID
Q9UK97
Alternative Names
F-Box Protein 9; Renal Carcinoma Antigen NY-REN-57; Cross-Immune Reaction Antigen 1; F-Box Only Protein 9; VCIA1; FBX9; F-Box Protein Fbx9; DJ341E18.2; NY-REN-57;
Research Area
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1.
Biological Process
Fat cell differentiation Source: Ensembl
Innate immune response Source: Ensembl
Protein ubiquitination Source: UniProtKB
Regulation of TOR signaling Source: UniProtKB
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
Cellular Location
Cytoplasm

Wang, Z., Chen, X., Zhou, L., Zhao, X., Ge, C., Zhao, F., ... & Li, J. (2022). FBXO9 Mediates the Cancer-Promoting Effects of ZNF143 by Degrading FBXW7 and Facilitates Drug Resistance in Hepatocellular Carcinoma. Frontiers in oncology, 12, 930220-930220.

Qu, X., Sun, Z., Wang, Y., & Ong, H. S. (2021). Zoledronic acid promotes osteoclasts ferroptosis by inhibiting FBXO9-mediated p53 ubiquitination and degradation. PeerJ, 9, e12510.

Liu, J. A., Tai, A., Hong, J., Cheung, M. P. L., Sham, M. H., Cheah, K. S., ... & Cheung, M. (2020). Fbxo9 functions downstream of Sox10 to determine neuron-glial fate choice in the dorsal root ganglia through Neurog2 destabilization. Proceedings of the National Academy of Sciences, 117(8), 4199-4210.

Hynes-Smith, R. W., Swenson, S. A., Vahle, H., Wittorf, K. J., Caplan, M., Amador, C., ... & Buckley, S. M. (2019). Loss of FBXO9 enhances proteasome activity and promotes aggressiveness in acute myeloid leukemia. Cancers, 11(11), 1717.

Hynes-Smith, R. (2019). The role of E3 ubiquitin ligase FBXO9 in normal and malignant hematopoiesis.

Hynes-Smith, R. W., Swenson, S., Wittorf, K., Amador, C., Woods, N., Hyde, K., & Buckley, S. (2018). Loss of FBXO9 Accelerates the Onset of Acute Myeloid Leukemia. Experimental Hematology, 64, S49.

Zou, C., Lai, Y., Li, X., & Li, J. (2018). LPS Reduces SCF-Fbxo9 to Elevate PRMT4 Protein Levels in Lymphocytes in an Acute Lung Injury Model. In A69. NOVEL SIGNALING CASCADES IN LUNG INJURY, INFLAMMATION, AND REPAIR (pp. A2248-A2248). American Thoracic Society.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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