Sign in or Register   Sign in or Register
  |  

Rabbit Anti-FFAR1 Recombinant Antibody (EG1188) (CBMAB-EN1433-LY)

The product is antibody recognizes FFAR1. The antibody EG1188 immunoassay techniques such as: WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000.
See all FFAR1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human
Clone
EG1188
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of humanFFAR1.
Specificity
Human
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Free Fatty Acid Receptor 1
Introduction
This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes. [provided by RefSeq, Apr 2009]
Entrez Gene ID
2864
UniProt ID
O14842
Alternative Names
Free Fatty Acid Receptor 1; G-Protein Coupled Receptor 40; GPR40; G Protein-Coupled Receptor 40; GPCR40; FFA1R;
Research Area
G-protein coupled receptor for medium and long chain saturated and unsaturated fatty acids that plays an important role in glucose homeostasis. Fatty acid binding increases glucose-stimulated insulin secretion, and may also enhance the secretion of glucagon-like peptide 1 (GLP-1). May also play a role in bone homeostasis; receptor signaling activates pathways that inhibit osteoclast differentiation (By similarity).

Ligand binding leads to a conformation change that triggers signaling via G-proteins that activate phospholipase C, leading to an increase of the intracellular calcium concentration. Seems to act through a G(q) and G(i)-mediated pathway. Mediates the anti-inflammatory effects of omega-3 polyunsaturated fatty acids (PUFAs) via inhibition of NLRP3 inflammasome activation.
Biological Process
Glucose homeostasis Source: Ensembl
G protein-coupled receptor signaling pathway Source: ProtInc
Insulin secretion Source: Ensembl
Negative regulation of interleukin-1 beta production Source: UniProtKB
Positive regulation of calcium ion transport Source: UniProtKB
Positive regulation of cytosolic calcium ion concentration Source: UniProtKB
Positive regulation of insulin secretion Source: Ensembl
Response to fatty acid Source: UniProtKB
Cellular Location
Cell membrane
Topology
Extracellular: 1-8
Helical: 9-31
Cytoplasmic: 32-41
Helical: 42-64
Extracellular: 65-79
Helical: 80-101
Cytoplasmic: 102-121
Helical: 122-142
Extracellular: 143-178
Helical: 179-200
Cytoplasmic: 201-223
Helical: 224-248
Extracellular: 249-256
Helical: 257-279
Cytoplasmic: 280-300

Teng, D., Zhou, Y., Tang, Y., Liu, G., & Tu, Y. (2022). Mechanistic Studies on the Stereoselectivity of FFAR1 Modulators. Journal of Chemical Information and Modeling, 62(15), 3664-3675.

Lückmann, M., Shenol, A., Nissen, T. A., Petersen, J. E., Kouvchinov, D., Schwartz, T. W., & Frimurer, T. M. (2022). Optimization of First-in-Class Dual-Acting FFAR1/FFAR4 Allosteric Modulators with Novel Mode of Action. ACS Medicinal Chemistry Letters.

Governa, P., Caroleo, M. C., Carullo, G., Aiello, F., Cione, E., & Manetti, F. (2021). FFAR1/GPR40: One target, different binding sites, many agonists, no drugs, but a continuous and unprofitable tug-of-war between ligand lipophilicity, activity, and toxicity. Bioorganic & Medicinal Chemistry Letters, 41, 127969.

Bazydlo-Guzenda, K., Buda, P., Mach, M., Pieczykolan, J., Kozlowska, I., Janiszewski, M., ... & Gad, S. C. (2021). Evaluation of the hepatotoxicity of the novel GPR40 (FFAR1) agonist CPL207280 in the rat and monkey. Plos one, 16(9), e0257477.

Li, Z., Zhou, Z., & Zhang, L. (2020). Current status of GPR40/FFAR1 modulators in medicinal chemistry (2016–2019): a patent review. Expert opinion on therapeutic patents, 30(1), 27-38.

Teng, D., Chen, J., Li, D., Wu, Z., Li, W., Tang, Y., & Liu, G. (2020). Computational insights into molecular activation and positive cooperative mechanisms of FFAR1 modulators. Journal of Chemical Information and Modeling, 60(6), 3214-3230.

Lückmann, M., Trauelsen, M., Bentsen, M. A., Nissen, T. A., Martins, J., Fallah, Z., ... & Frimurer, T. M. (2019). Molecular dynamics-guided discovery of an ago-allosteric modulator for GPR40/FFAR1. Proceedings of the National Academy of Sciences, 116(14), 7123-7128.

Marcial-Medina, C., Ordoñez-Moreno, A., Gonzalez-Reyes, C., Cortes-Reynosa, P., & Salazar, E. P. (2019). Oleic acid induces migration through a FFAR1/4, EGFR and AKT-dependent pathway in breast cancer cells. Endocrine Connections, 8(3), 252-265.

Zhang, X., Sun, H., Wen, X., & Yuan, H. (2019). A selectivity study of FFAR4/FFAR1 Agonists by molecular modeling. Journal of chemical information and modeling, 59(10), 4467-4474.

Wang, J., Hong, Y., Shao, S., Zhang, K., & Hong, W. (2018). FFAR1-and FFAR4-dependent activation of Hippo pathway mediates DHA-induced apoptosis of androgen-independent prostate cancer cells. Biochemical and biophysical research communications, 506(3), 590-596.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rabbit Anti-FFAR1 Recombinant Antibody (EG1188)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare