Rabbit Anti-GNAI3 Recombinant Antibody (EG1336) (CBMAB-EN1614-LY)

Name: Rabbit Anti-GNAI3 Recombinant Antibody (EG1336)
SKU: CBMAB-EN1614-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

EG1336

Application

WB, IHC-P, IF

Immunogen

Recombinant fragment of human GNAI3.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

G protein subunit alpha i3

Introduction

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway.

Entrez Gene ID

Human	2773
Mouse	14679
Rat	25643

UniProt ID

Human	P08754
Mouse	Q9DC51
Rat	P08753

Alternative Names

87U6; ARCND1

Function

Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541, PubMed:19478087).

Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087).

Stimulates the activity of receptor-regulated K+ channels (PubMed:2535845).

The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935).

Biological Process

Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: UniProtKB
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
Brain development Source: Ensembl
Cell cycle Source: UniProtKB-KW
Cell division Source: UniProtKB
Dopamine receptor signaling pathway Source: UniProtKB
GTP metabolic process Source: UniProtKB
Negative regulation of adenylate cyclase activity Source: ProtInc
Negative regulation of apoptotic signaling pathway Source: Ensembl
Positive regulation of macroautophagy Source: UniProtKB
Positive regulation of NAD(P)H oxidase activity Source: Ensembl
positive regulation of superoxide anion generation Source: Ensembl
Positive regulation of vascular associated smooth muscle cell proliferation Source: Ensembl
Vesicle fusion Source: Ensembl

Cellular Location

Cell membrane; Cytoplasm; Centrosome. Localizes in the centrosomes of interphase and mitotic cells. Detected at the cleavage furrow and/or the midbody.

Involvement in disease

Auriculocondylar syndrome 1 (ARCND1):
An autosomal dominant craniofacial malformation syndrome characterized by variable mandibular anomalies, including mild to severe micrognathia, temporomandibular joint ankylosis, cleft palate, and a characteristic ear malformation that consists of separation of the lobule from the external ear, giving the appearance of a question mark (question-mark ear). Other frequently described features include prominent cheeks, cupped and posteriorly rotated ears, preauricular tags, and microstomia.

PTM

(Microbial infection) Deamidated at Gln-204 by Photorhabdus asymbiotica toxin PAU_02230, blocking GTP hydrolysis of heterotrimeric GNAQ or GNA11 and G-alphai (GNAI1, GNAI2 or GNAI3) proteins, thereby activating RhoA.

Leiss, V., Reisinger, E., Speidel, A., Beer-Hammer, S., & Nürnberg, B. (2021). Analyses of Gnai3-iresGFP reporter mice reveal unknown Gαi3 expression sites. Scientific Reports, 11(1), 14271.

Mishra, C., & Mohapatra, S. (2020). An integrative bioinformatics analysis of caprine GNAI3 gene. Journal of Genetics, 99, 1-7.

Li, Z. W., Sun, B., Gong, T., Guo, S., Zhang, J., Wang, J., ... & Chu, W. M. (2019). GNAI1 and GNAI3 reduce colitis-associated tumorigenesis in mice by blocking IL6 signaling and down-regulating expression of GNAI2. Gastroenterology, 156(8), 2297-2312.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-GNAI3 Recombinant Antibody (CBLG1-1409) (CAT#: V2LY-0226-LY147)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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