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Rabbit Anti-GNAT1 Recombinant Antibody (EG1338) (CBMAB-EN1616-LY)

The product is antibody recognizes GNAT1. The antibody EG1338 immunoassay techniques such as: WB: 1:500~1:1000 ELISA: 1:40000.
See all GNAT1 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse
Clone
EG1338
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 ELISA: 1:40000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human GNAT1.
Specificity
Human, Mouse
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G protein subunit alpha transducin 1
Introduction
Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Entrez Gene ID
Human2779
Mouse14685
UniProt ID
HumanP11488
MouseP20612
Alternative Names
GBT1; GNATR; CSNB1G; CSNBAD3
Function
Functions as signal transducer for the rod photoreceptor RHO. Required for normal RHO-mediated light perception by the retina (PubMed:22190596).

Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs), such as the photoreceptor RHO. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Activated RHO promotes GDP release and GTP binding. Signaling is mediated via downstream effector proteins, such as cGMP-phosphodiesterase (By similarity).
Biological Process
Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: GO_Central
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
Cell population proliferation Source: Ensembl
Cellular response to electrical stimulus Source: Ensembl
Detection of chemical stimulus involved in sensory perception of bitter taste Source: Ensembl
Detection of light stimulus involved in visual perception Source: UniProtKB
Eye photoreceptor cell development Source: Ensembl
G protein-coupled adenosine receptor signaling pathway Source: GO_Central
Negative regulation of cyclic-nucleotide phosphodiesterase activity Source: UniProtKB
Phototransduction, visible light Source: UniProtKB
Positive regulation of cyclic-nucleotide phosphodiesterase activity Source: Ensembl
Regulation of rhodopsin mediated signaling pathway Source: Reactome
Response to light intensity Source: Ensembl
Response to light stimulus Source: UniProtKB
Retina development in camera-type eye Source: Ensembl
Rhodopsin mediated signaling pathway Source: Ensembl
Sensory perception of umami taste Source: Ensembl
Signal transduction Source: UniProtKB
Visual perception Source: UniProtKB
Cellular Location
Photoreceptor outer segment; Membrane; Photoreceptor inner segment. Localizes mainly in the outer segment in the dark-adapted state, whereas is translocated to the inner part of the photoreceptors in the light-adapted state. During dark-adapted conditions, in the presence of UNC119 mislocalizes from the outer segment to the inner part of rod photoreceptors which leads to decreased photoreceptor damage caused by light.
Involvement in disease
Night blindness, congenital stationary, autosomal dominant 3 (CSNBAD3):
A non-progressive retinal disorder characterized by impaired night vision, often associated with nystagmus and myopia.
Night blindness, congenital stationary, 1G (CSNB1G):
An autosomal recessive form of congenital stationary night blindness, a non-progressive retinal disorder characterized by impaired night vision or in dim light, with good vision only on bright days.

Schoonderwoerd, R. A., Buck, T. M., Andriessen, C. A., Wijnholds, J., Hattar, S., Meijer, J. H., & Deboer, T. (2022). Sleep Deprivation Does not Change the Flash Electroretinogram in Wild-type and Opn4−/− Gnat1−/− Mice. Journal of biological rhythms, 37(2), 216-221.

Hayashi, T., Hosono, K., Kurata, K., Katagiri, S., Mizobuchi, K., Ueno, S., ... & Hotta, Y. (2020). Coexistence of GNAT1 and ABCA4 variants associated with Nougaret-type congenital stationary night blindness and childhood-onset cone-rod dystrophy. Documenta Ophthalmologica, 140, 147-157.

Wang, J., Wang, W., Tang, Q., Lu, L., Luo, Z., Li, W., ... & Pu, J. (2020). Long Non-coding RNA lnc-GNAT1-1 Suppresses Liver Cancer Progression via Modulation of Epithelial–Mesenchymal Transition. Frontiers in Genetics, 11, 1029.

Zeitz, C., Méjécase, C., Stévenard, M., Michiels, C., Audo, I., & Marmor, M. F. (2018). A novel heterozygous missense mutation in GNAT1 leads to autosomal dominant Riggs type of congenital stationary night blindness. BioMed Research International, 2018.

Marmor, M. F., & Zeitz, C. (2018). Riggs-type dominant congenital stationary night blindness: ERG findings, a new GNAT1 mutation and a systemic association. Documenta Ophthalmologica, 137, 57-62.

Liu, L., Shuai, T., Li, B., Zhu, L., & Li, X. (2018). Long non‑coding RNA lnc‑GNAT1‑1 inhibits gastric cancer cell proliferation and invasion through the Wnt/β‑catenin pathway in Helicobacter pylori infection. Molecular Medicine Reports, 18(4), 4009-4015.

Zahid, S., Branham, K., Schlegel, D., Pennesi, M. E., Michaelides, M., Heckenlively, J., ... & Jayasundera, T. (2018). GNAT1. Retinal Dystrophy Gene Atlas, 101-101.

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For research use only. Not intended for any clinical use.

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