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Mouse Anti-GNMT Recombinant Antibody (CBFYH-0406) (CBMAB-H1276-FY)

This product is mouse antibody that recognizes GNMT. The antibody CBFYH-0406 can be used for immunoassay techniques such as: WB, FC.
See all GNMT antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-0406
Antibody Isotype
IgG3
Application
WB, FC

Basic Information

Immunogen
Full length human recombinant protein of human GNMT produced in HEK293T cell
Specificity
Human
Antibody Isotype
IgG3
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Glycine N-Methyltransferase
Introduction
The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. This protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). Alternative splicing results in multiple transcript variants. Naturally occurring readthrough transcription occurs between the upstream CNPY3 (canopy FGF signaling regulator 3) gene and this gene and is represented with GeneID:107080644.
Entrez Gene ID
27232
UniProt ID
Q14749
Alternative Names
Glycine N-Methyltransferase; EC 2.1.1.20; Epididymis Secretory Sperm Binding Protein Li 182mP; HEL-S-182mP
Function
Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.
Biological Process
Cellular protein modification process Source: UniProtKB
Glycogen metabolic process Source: Ensembl
Methionine metabolic process Source: Ensembl
Methylation Source: UniProtKB-KW
One-carbon metabolic process Source: GO_Central
Protein homotetramerization Source: UniProtKB
Regulation of gluconeogenesis Source: GO_Central
S-adenosylhomocysteine metabolic process Source: GO_Central
S-adenosylmethionine metabolic process Source: UniProtKB
Sarcosine metabolic process Source: GO_Central
Cellular Location
Cytoplasm
Involvement in disease
Glycine N-methyltransferase deficiency (GNMT deficiency):
The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases.

Simile, M. M., Cigliano, A., Paliogiannis, P., Daino, L., Manetti, R., Feo, C. F., ... & Pascale, R. M. (2022). Nuclear localization dictates hepatocarcinogenesis suppression by glycine N-methyltransferase. Translational Oncology, 15(1), 101239.

Zabala-Letona, A., Arruabarrena-Aristorena, A., Fernandez-Ruiz, S., Viera, C., Carlevaris, O., Ercilla, A., ... & Carracedo, A. (2022). PI3K-regulated Glycine N-methyltransferase is required for the development of prostate cancer. Oncogenesis, 11(1), 10.

Rome, F. I., & Hughey, C. C. (2022). Disrupted liver oxidative metabolism in glycine N-methyltransferase-deficient mice is mitigated by dietary methionine restriction. Molecular Metabolism, 58, 101452.

Tain, L. S., Jain, C., Nespital, T., Froehlich, J., Hinze, Y., Grönke, S., & Partridge, L. (2020). Longevity in response to lowered insulin signaling requires glycine N‐methyltransferase‐dependent spermidine production. Aging Cell, 19(1), e13043.

Borowa-Mazgaj, B., de Conti, A., Tryndyak, V., Steward, C. R., Jimenez, L., Melnyk, S., ... & Pogribny, I. P. (2019). Gene expression and DNA methylation alterations in the glycine N-methyltransferase gene in diet-induced nonalcoholic fatty liver disease-associated carcinogenesis. Toxicological Sciences, 170(2), 273-282.

Chen, M., Yang, M. H., Chang, M. M., Tyan, Y. C., & Chen, Y. M. A. (2019). Tumor suppressor gene glycine N-methyltransferase and its potential in liver disorders and hepatocellular carcinoma. Toxicology and Applied Pharmacology, 378, 114607.

Hung, J. H., Li, C. H., Yeh, C. H., Huang, P. C., Fang, C. C., Chen, Y. F., ... & Chen, Y. M. A. (2018). MicroRNA-224 down-regulates Glycine N-methyltransferase gene expression in Hepatocellular Carcinoma. Scientific Reports, 8(1), 1-14.

Simile, M. M., Latte, G., Feo, C. F., Feo, F., Calvisi, D. F., & Pascale, R. M. (2018). Alterations of methionine metabolism in hepatocarcinogenesis: the emergent role of glycine N-methyltransferase in liver injury. Annals of Gastroenterology, 31(5), 552.

Hughey, C. C., Trefts, E., Bracy, D. P., James, F. D., Donahue, E. P., & Wasserman, D. H. (2018). Glycine N-methyltransferase deletion in mice diverts carbon flux from gluconeogenesis to pathways that utilize excess methionine cycle intermediates. Journal of Biological Chemistry, 293(30), 11944-11954.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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