Mouse Anti-GOLGA7 Recombinant Antibody (CBLG1-1487) (CBMAB-G4287-LY)

This product is antibody recognizes GOLGA7. The antibody CBLG1-1487 immunoassay techniques such as: IP, S-ELISA, ELISA, WB.
See all GOLGA7 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

CBLG1-1487

Antibody Isotype

IgG2a, κ

Application

IP, S-ELISA, ELISA, WB

Basic Information

Immunogen

GOLGA7 (AAH12032, 1 a.a. ~ 137 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

golgi autoantigen, golgin subfamily a, 7

Entrez Gene ID

51125

UniProt ID

Q7Z5G4

Alternative Names

GCP16; GOLGA3AP1A; HSPC041; GOLGA7

Function

May be involved in protein transport from Golgi to cell surface. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.

Biological Process

Golgi to plasma membrane protein transport Source: UniProtKB
Golgi to plasma membrane transport Source: MGI
Peptidyl-L-cysteine S-palmitoylation Source: UniProtKB
Protein stabilization Source: UniProtKB
Protein targeting to membrane Source: GO_Central

Cellular Location

Golgi apparatus membrane

PTM

Palmitoylated on Cys-69 and Cys-72; which is required for Golgi localization and interaction with GOLGA3.

References

Zeng, X. T., Yu, X. X., & Cheng, W. (2022). Correction to: The interactions of ZDHHC5/GOLGA7 with SARS‑CoV‑2 spike (S) protein and their effects on S protein’s subcellular localization, palmitoylation and pseudovirus entry. Virology Journal, 19(1), 25-25.

Zeng, X. T., Yu, X. T., & Cheng, W. (2021). The interactions of ZDHHC5/GOLGA7 with SARS-CoV-2 spike (S) protein and their effects on S protein’s subcellular localization, palmitoylation and pseudovirus entry. Virology Journal, 18, 1-16.

Ko, P. J., Woodrow, C., Dubreuil, M. M., Martin, B. R., Skouta, R., Bassik, M. C., & Dixon, S. J. (2019). A ZDHHC5-GOLGA7 protein acyltransferase complex promotes nonapoptotic cell death. Cell chemical biology, 26(12), 1716-1724.

Zhou, L., Dong, S., Deng, Y., Yang, P., Zheng, Y., Yao, L., ... & Dai, Z. (2019). GOLGA7 rs11337, a polymorphism at the microRNA binding site, is associated with glioma prognosis. Molecular Therapy-Nucleic Acids, 18, 56-65.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

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Mouse Anti-GOLGA7 Recombinant Antibody (CBLG1-1483)

Mouse Anti-GOLGA7 Recombinant Antibody (CBLG1-1486)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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