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Rabbit Anti-GPR158 Recombinant Antibody (EG1388) (CBMAB-EN1667-LY)

The product is antibody recognizes GPR158. The antibody EG1388 immunoassay techniques such as: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:500~1:1000 ELISA: 1:5000.
See all GPR158 antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse
Clone
EG1388
Antibody Isotype
IgG
Application
WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:500~1:1000 ELISA: 1:5000

Basic Information

Immunogen
The antibody was produced against synthesized peptide derived from internal of human GPR158.
Specificity
Human, Mouse
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
G protein-coupled receptor 158
Entrez Gene ID
Human57512
Mouse241263
UniProt ID
HumanQ5T848
MouseQ8C419
Function
Orphan receptor.
Biological Process
Protein localization to plasma membrane Source: Ensembl
Cellular Location
Cell membrane
Topology
Extracellular: 24-417
Helical: 418-438
Cytoplasmic: 439-451
Helical: 452-472
Extracellular: 473-483
Helical: 484-506
Cytoplasmic: 507-528
Helical: 529-549
Extracellular: 550-579
Helical: 580-600
Cytoplasmic: 601-613
Helical: 614-634
Extracellular: 635-643
Helical: 644-664
Cytoplasmic: 665-1215

Chang, J., Song, Z., Wei, S., Zhou, Y., Ju, J., Yao, P., ... & Li, N. (2023). Expression Mapping and Functional Analysis of Orphan G-Protein-Coupled Receptor GPR158 in the Adult Mouse Brain Using a GPR158 Transgenic Mouse. Biomolecules, 13(3), 479.

Wei, S., Zheng, L., Chang, J., Jiang, J., Zhou, Z., Liao, H., ... & Li, N. (2023). G protein‐coupled receptor 158 modulates sensitivity to the sedative‐hypnotic effect of ethanol in male mice. Alcoholism: Clinical and Experimental Research.

da Silva, E. P., da Silva Feltran, G., Dos Santos, S. A. A., de Oliveira, R. C., Assis, R. I., Junior, L. A. J., ... & da Silva, R. A. F. (2023). Hyperglycemic microenvironment compromises the homeostasis of communication between the bone-brain axis by the epigenetic repression of the osteocalcin receptor, Gpr158 in the hippocampus. Brain Research, 148234.

Fu, X., Wei, S., Wang, T., Fan, H., Zhang, Y., Costa, C. D., ... & Li, N. (2022). Research status of the orphan G protein coupled receptor 158 and future perspectives. Cells, 11(8), 1334.

Watkins, L. R., & Orlandi, C. (2021). In vitro profiling of orphan G protein coupled receptor (GPCR) constitutive activity. British Journal of Pharmacology, 178(15), 2963-2975.

Gonçalves-Monteiro, S., Ribeiro-Oliveira, R., Vieira-Rocha, M. S., Vojtek, M., Sousa, J. B., & Diniz, C. (2021). Insights into nuclear G-protein-coupled receptors as therapeutic targets in non-communicable diseases. Pharmaceuticals, 14(5), 439.

Watkins, L. R., & Orlandi, C. (2020). Orphan G protein coupled receptors in affective disorders. Genes, 11(6), 694.

Engqvist, H., Parris, T. Z., Kovács, A., Nemes, S., Werner Rönnerman, E., De Lara, S., ... & Helou, K. (2019). Immunohistochemical validation of COL3A1, GPR158 and PITHD1 as prognostic biomarkers in early-stage ovarian carcinomas. BMC cancer, 19, 1-12.

Song, C., Orlandi, C., Sutton, L. P., & Martemyanov, K. A. (2019). The signaling proteins GPR158 and RGS7 modulate excitability of L2/3 pyramidal neurons and control A-type potassium channel in the prelimbic cortex. Journal of Biological Chemistry, 294(35), 13145-13157.

Dunn, H. A., Orlandi, C., & Martemyanov, K. A. (2019). Beyond the ligand: Extracellular and transcellular G protein–coupled receptor complexes in physiology and pharmacology. Pharmacological Reviews, 71(4), 503-519.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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