Mouse Anti-GPR183 Recombinant Antibody (SA313E4) (CBMAB-G0710-LY)

Mouse

Human

SA313E4

IgG2a, κ

FC

Human GPR183-transfected cells

Human

IgG2a, κ

Monoclonal

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Liquid

0.2% (w/v) BSA

0.09% sodium azide

mg/mL

> 95% Purity determined by SDS-PAGE.

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

G Protein-Coupled Receptor 183

This gene was identified by the up-regulation of its expression upon Epstein-Barr virus infection of primary B lymphocytes. This gene is predicted to encode a G protein-coupled receptor that is most closely related to the thrombin receptor. Expression of this gene was detected in B-lymphocyte cell lines and lymphoid tissues but not in T-lymphocyte cell lines or peripheral blood T lymphocytes. The function of this gene is unknown. [provided by RefSeq, Jul 2008]

G Protein-Coupled Receptor 183; EBV-Induced G-Protein Coupled Receptor 2; Epstein-Barr Virus Induced Gene 2 (Lymphocyte-Specific G Protein-Coupled Receptor); Epstein-Barr Virus-Induced G-Protein Coupled Receptor 2; HEBI2; EBI2; G-Protein Coupled Receptor 183;

G-protein coupled receptor expressed in lymphocytes that acts as a chemotactic receptor for B-cells, T-cells, splenic dendritic cells, monocytes/macrophages and astrocytes (By similarity).

Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols (PubMed:21796212, PubMed:22875855, PubMed:22930711).

Mediates cell positioning and movement of a number of cells by binding the 7-alpha,25-OHC ligand that forms a chemotactic gradient (By similarity).

Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses (By similarity).

Guides B-cell movement along the B-cell zone-T-cell zone boundary and later to interfollicular and outer follicular regions (By similarity).

Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses (By similarity).

Collaborates with CXCR5 to mediate B-cell migration; probably by forming a heterodimer with CXCR5 that affects the interaction between of CXCL13 and CXCR5 (PubMed:22913878).

Also acts as a chemotactic receptor for some T-cells upon binding to 7-alpha,25-OHC ligand (By similarity).

Promotes follicular helper T (Tfh) cells differentiation by positioning activated T-cells at the follicle-T-zone interface, promoting contact of newly activated CD4 T-cells with activated dendritic cells and exposing them to Tfh-cell-promoting inducible costimulator (ICOS) ligand (By similarity).

Expression in splenic dendritic cells is required for their homeostasis, localization and ability to induce B- and T-cell responses: GPR183 acts as a chemotactic receptor in dendritic cells that mediates the accumulation of CD4+ dendritic cells in bridging channels (By similarity).

Regulates migration of astrocytes and is involved in communication between astrocytes and macrophages (PubMed:25297897).

Promotes osteoclast precursor migration to bone surfaces (By similarity).

Signals constitutively through G(i)-alpha, but not G(s)-alpha or G(q)-alpha (PubMed:21673108, PubMed:25297897).

Signals constitutively also via MAPK1/3 (ERK1/2) (By similarity).

Adaptive immune response Source: UniProtKB
B cell activation involved in immune response Source: GO_Central
Dendritic cell chemotaxis Source: UniProtKB
Dendritic cell homeostasis Source: UniProtKB
G protein-coupled receptor signaling pathway Source: UniProtKB
Humoral immune response Source: UniProtKB
Immune response Source: ProtInc
Leukocyte chemotaxis Source: UniProtKB
Mature B cell differentiation involved in immune response Source: UniProtKB
Osteoclast differentiation Source: UniProtKB
Positive regulation of B cell proliferation Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
Regulation of astrocyte chemotaxis Source: UniProtKB
T cell chemotaxis Source: UniProtKB
T follicular helper cell differentiation Source: UniProtKB

Cell membrane

Extracellular: 1-31
Helical: 32-57
Cytoplasmic: 58-77
Helical: 78-95
Extracellular: 96-105
Helical: 106-127
Cytoplasmic: 128-149
Helical: 150-168
Extracellular: 169-192
Helical: 193-215
Cytoplasmic: 216-241
Helical: 242-265
Extracellular: 266-287
Helical: 288-312
Cytoplasmic: 313-361